Duplex PCR method for rapidly identifying goose parvovirus and cherry valley duck source parvovirus

Abstract

The invention relates to a duplex PCR method for rapidly identifying the goose parvovirus and the cherry valley duck source parvovirus. New specific primers are designed and screened, various parameters in the reaction process are optimized, and the duplex PCR method, established with the group of primers, for the goose parvovirus and the cherry valley duck source parvovirus is high in specificity and sensibility and capable of being used for rapidly and accurately identifying the goose parvovirus and the new cherry valley duck source parvovirus. The established duplex PCR method is good in repeatability, high in reliability, low in cost, easy to implement and suitable for large-scale epidemiological investigation. It is proved through detection of 1500 samples that the duplex PCR method has high reliability; besides, identification can be carried out just through a common PCR instrument, the cost of primer synthesis is very low, and thus the cost of a reaction in the duplex PCR method is almost the same as that of a common PCR; experiment operation is also easy, and the duplex PCR method is suitable for the present production fact of the animal husbandry in China.

Description

(1) Technical field [0001] The invention relates to a double PCR method for quickly identifying goose parvovirus and cherry valley duck parvovirus, belonging to the field of virus molecular biology. (2) Background technology [0002] Goose parvovirus (Gooseparvovirus, GPV) is one of the members of Parvoviridae, which can cause disease in goslings and muscovy ducks, and produce lesions characterized by the necrosis and shedding of the surface of the small intestinal mucosa and the formation of small intestinal embolism, with high morbidity and mortality . According to previous studies, the natural infection hosts of parvovirus in waterfowl are only geese and Muscovy ducks, and there are no reports of it infecting other species of ducks. However, since 2015, there have been reports in my country that Cherry Valley meat ducks have symptoms such as unexplained short beak, protruding tongue, slow growth, and weight loss. Clinical autopsy showed enlarged thymus, obvious bleeding ...

AI Technical Summary

Problems solved by technology

At present, PCR, fluorescent quantitative PCR, double PCR methods for detection of traditional goose parvovirus and Muscovy duck parvovirus have been established, but there is no relevant report on the detection of traditional goose parvovirus and this new type of goose parvovirus (Cherry Valley Therefore, there is an urgent need for a method with good specificity and high sensitivity to detect the mixed infection of traditional goose parvovirus and the new goose parvovirus

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