Preparation and application of efficient cell inactivation vaccine against new duck reovirus disease

A technology for reovirus disease and inactivated vaccine, which is applied in the field of high-efficiency cell inactivated vaccine for new duck reovirus disease to achieve a highly practical effect

Pending Publication Date: 2016-08-17
SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no effective control method for this disease

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0024] In this example, the duck reovirus NDRV-TH11 strain was used to inoculate the monolayer of duck embryo fibroblasts immortalized in our laboratory, and the cytotoxicity was harvested when the cytopathy reached 80%; After the ester (BPL) is inactivated, it is mixed with a self-made nano adjuvant in an appropriate proportion to make a high-efficiency cell inactivated vaccine for new duck reovirus disease, and then the above inactivated vaccine is used to prevent new duck reovirus disease; breeding duck immunization and neutralizing antibody: take the inactivated vaccine to immunize breeding ducks once, 10ml / feather; 4 weeks later booster immunization once, 10ml / feather. The duck reovirus NDRV-TH11 strain is preserved in the China General Microbiological Culture Collection Center (CGMCC for short), and the depository unit is CGMCC. The address is at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, China. The deposit date was November 28, 2013. The deposit n...

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Abstract

The invention relates to preparation and application of an efficient cell inactivation vaccine against new duck reovirus disease. The preparation method comprises the following steps: an antigen preparation step: inoculating a virus NDRV-TH11 strain with immortalized duck embryo fibroblast to obtain cytotoxicity with 80% of cytopathic effect, and performing cryopreservation at -20 DEG C; and a vaccine preparation step: inactivating the obtained cytotoxicity and mixing with a homemade nano adjuvant and stirring uniformly to obtain the efficient cell inactivation vaccine against new duck reovirus disease. After the duck is immunized by the efficient cell inactivation vaccine against new duck reovirus disease prepared in the invention, the duck can effectively resist the attack of a virulent strain of the new duck reovirus.

Description

technical field [0001] The invention relates to the preparation and application of a high-efficiency cell-inactivated vaccine for emerging duck reovirus disease, in particular to a high-efficiency cell-inactivated vaccine for emerging duck reovirus disease and its role in preventing emerging duck reovirus disease The application on viral diseases belongs to the field of preventive veterinary medicine. Background technique [0002] Avian reovirus infection of waterfowl was first reported only in Muscovy ducks, called Muscovy duck reovirus disease, and its causative agent was called Muscovy duck reovirus (MDRV). Studies have shown that MDRV is not infectious to Peking ducks and other local breeds of ducks in my country. With the continuous development of the duck industry, reovirus infection of many breeds of ducks has also been reported. However, due to the differences in endemic areas, disease populations and disease time, reovirus infection of ducks shows different degrees...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/15A61P31/14
CPCA61K39/12A61K2039/5252A61K2039/545A61K2039/552C12N2720/12034
Inventor 陈宗艳刘光清李传峰
Owner SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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