Microbial inoculum for improving water body and structure of biological intestinal colony, and preparation method and application thereof

An intestinal bacteria and biological technology, applied in the biological field, can solve the problem of not being able to improve the aquaculture environment and the intestinal environment of aquaculture organisms at the same time, and achieve the effects of increasing the survival rate, increasing the growth rate, and improving the breeding environment.

Inactive Publication Date: 2010-04-07
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The primary purpose of the present invention is to overcome the deficiency that the existing microbial preparations cannot simultaneously improve the aquaculture environment and...

Method used

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  • Microbial inoculum for improving water body and structure of biological intestinal colony, and preparation method and application thereof
  • Microbial inoculum for improving water body and structure of biological intestinal colony, and preparation method and application thereof
  • Microbial inoculum for improving water body and structure of biological intestinal colony, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Preparation of Bdellovibrio bacterial liquid

[0034] The Bdellovibrio bacterium liquid is prepared by the fermentation method of the high-density Bdellovibrio swimming body disclosed in the national invention patent application with the application number "200710031166.X": respectively in 3 tanks with 100ml LB (Luria-Bertani) liquid medium Inoculate Aeromonas hydrophila (Aeromonas hydrophila, purchased from Guangdong Provincial Institute of Microbiology Culture Collection Center, No. GIM1.172) in the Erlenmeyer flask, 250rpm, 30°C shaker culture for 24 hours, and the culture solution was separated at 4°C, 5000rpm Centrifuge for 15 minutes and discard the supernatant. Add the precipitates to 3 mediums containing 100ml DNB liquid medium (nutrient broth 0.8g, casein acid hydrolyzate 0.5g, yeast extract 0.1g, NaCl 30g, dissolve in 1000ml distilled water, pH value is 7.2~7.6 ) in Erlenmeyer flasks, and then pick bacterial plaques from the double-layer plates and insert...

Embodiment 2

[0058] (1) Preparation of Bdellovibrio bacterial liquid

[0059] Same as step (1) of Example 1, the only difference is that the Bdellovibrio sediment is suspended with a 0.05M pH value of 7.2 sterile phosphate buffer solution, Bdellovibrio BDF01 bacterium liquid, Bdellovibrio BDF02 bacterium liquid and Bdellovibrio bacterium The concentration of BDM01 bacterium liquid is respectively 10 7 pfu / ml.

[0060] (2) Preparation of beneficial bacteria liquid

[0061] Same as step (2) of Example 1, the difference is that strain 3y621 is used instead of strain H 2 . Adjust the concentration of the photosynthetic bacteria bacterium liquid with 0.05MpH as 7.2 aseptic phosphate buffer, be 10 5 cfu / ml; adjust the concentration of bacteria 3y621, Streptococcus lactis and Lactobacillus bulgaricus with physiological saline respectively, and the concentration is 107cfu / ml.

[0062] The beneficial bacteria above are mixed in equal proportions to obtain the beneficial bacteria agent.

[006...

Embodiment 3

[0080] The test of the effect of the bacterial agent on the cultured tilapia by improving the structure of the water body and the biological intestinal colony includes the following specific steps:

[0081] (1) Preparation of Bdellovibrio Concentrate

[0082] With embodiment 1 step (1), the only difference is that with sterile water suspension Bdellovibrio sediment, the concentrations of Bdellovibrio BDF01 bacterial liquid, Bdellovibrio BDF02 bacterial liquid and Bdellovibrio BDM01 bacterial liquid are respectively 10 10 pfu / ml.

[0083] (2) the preparation of probiotic bacterium liquid, with embodiment (2) step (2)

[0084] (3) Preparation of bacterial agents to improve water body and biological intestinal colony structure

[0085] The Bdellovibrio concentrated solution is mixed with the beneficial bacteria at a volume ratio of 1:4 to obtain a bacterial agent for improving water body and biological intestinal colony structure.

[0086] (4) the bacterial agent prepared by s...

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Abstract

The invention discloses a microbial inoculum for improving water body and structure of biological intestinal colony, and a preparation method and application thereof. In the method, bdellovibrio bacteriovorus., photosynthetic bacteria, streptococcus lactis, Lactobacillus bulgaricus and aquaculture biological intestinal beneficial strains are mixed to obtain the microbial inoculum for improving water body and structure of biological intestinal colony. The modified microbial inoculum is applied in fresh water aquaculture process, thereby effectively improving aquaculture environment and the structure of biological intestinal colony. The tropism and splitting capability of bdellovibrio bacteriovorus are utilized in the invention, and simultaneously the vacant niche left by pathogenic bacterium can be filled by the bacteria and other good bacteria which can be colonized in the intestinal canals of cultured organisms, thereby realizing the purpose of improving aquaculture environment and the structure of biological intestinal colony. The microbial inoculum of the invention has effectiveness, environment protection and no generation of pathogenic bacteria resistance, thereby having good application prospect.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a bacterial agent for improving water body and biological intestinal colony structure, a preparation method and application thereof. Background technique [0002] At present, the rapid development of the aquaculture industry and the increasing scale of intensive high-density aquaculture have led to an increase in the variety of pathogenic microorganisms and accelerated transmission, and the occurrence of aquaculture biological diseases has become increasingly serious, resulting in serious losses in the aquaculture industry. [0003] So far, in the disease prevention and treatment of aquaculture animals at home and abroad, antibiotics and other preparations are still mainly used. However, the extensive abuse of antibiotics will lead to some side effects, such as drug residues, drug resistance of pathogenic bacteria, inhibition of beneficial bacteria and so on. The...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34A61K35/74A61P1/00A01N63/02A01P1/00C12R1/01C12R1/46C12R1/225A61K35/741A61K35/747
Inventor 蔡俊鹏林珊宇
Owner SOUTH CHINA UNIV OF TECH
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