Use of UV induction mutation for reinforcing cracking performance of Bdellovibrio

A technology of Bdellovibrio and its performance, which is applied in the application field of ultraviolet-induced mutation in enhancing the lysis performance of Bdellovibrio, can solve the problems of difficult screening, long time, and difficult recovery of mutation frequency, etc., and achieves convenient operation, simple method, and improved The effect of lytic capacity
CN101440362AInactive Publication Date: 2009-05-27SOUTH CHINA UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SOUTH CHINA UNIV OF TECH
Publication Date
2009-05-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the technical field of mutation breeding of microorganisms, and particularly relates to application of ultraviolet-induced mutation in the strengthening pyrolysis performance of bdellovibrio bacteria. The application takes the ultraviolet-induced mutation as a main way and is assisted with an infrared light source and LiCl enhanced mutagenesis to perform induced mutation under the slow stirring, the time is between 2 and 8 minutes, and the radiation distance is between 5 and 20 centimeters; and the bdellovibrio bacteria is preserved under dark light and ice bath conditions and is accumulatively mutagenized until the requirement is achieved. The application has a simple method and convenient operation, has remarkable effect on strengthening the pyrolysis performance of the bdellovibrio bacteria, can pyrolyze two salmonella strains which fail to be pyrolyzed originally and a plurality of Gram-positive bacteria (including bacillus aerogenes capsulatus, clostridium difficile, streptococcus suis and enterococcus) proven by the experiment, solve the problems that usually the bdellovibrio bacteria can not pyrolyze the Gram-positive bacteria and the pyrolysis ability is extremely weak even if the bdellovibrio bacteria can pyrolyze the Gram-positive bacteria, and widen the application range of the bdellovibrio bacteria.
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Description

technical field

[0001] The invention belongs to the technical field of microbial mutation breeding, and in particular relates to the application of ultraviolet-induced mutation in enhancing the lysing performance of Bdellovibrio. Background technique

[0002] For microorganisms in nature, it is difficult to obtain excellent strains if they are simply isolated and purified, and the strains are selected, and generally it is difficult to meet the needs of production. Microbial breeding refers to the use of physical, chemical and other factors to induce variation in the genetic characteristics of microorganisms under artificial conditions, and to select excellent strains with special traits that meet the requirements for cultivation, so that microorganisms can be effectively used in industrial production.

[0003] The characteristics of Bdellovibrio that can infect and lyse host bacteria make it suitable as a biological decontamination factor for inhibiting or eliminating pathog...

Claims

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