Use of UV induction mutation for reinforcing cracking performance of Bdellovibrio

A technology of Bdellovibrio and its performance, which is applied in the application field of ultraviolet-induced mutation in enhancing the lysis performance of Bdellovibrio, can solve the problems of difficult screening, long time, and difficult recovery of mutation frequency, etc., and achieves convenient operation, simple method, and improved The effect of lytic capacity

Inactive Publication Date: 2009-05-27
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, at present, scholars at home and abroad mostly use the method of natural screening, which not only takes a long time, but also is difficult to obtain.
Ultraviolet mutagenesis

Method used

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  • Use of UV induction mutation for reinforcing cracking performance of Bdellovibrio
  • Use of UV induction mutation for reinforcing cracking performance of Bdellovibrio
  • Use of UV induction mutation for reinforcing cracking performance of Bdellovibrio

Examples

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Example Embodiment

[0026] Example 1

[0027] The application of UV-induced mutations, with the aid of infrared light sources and LiCl enhanced mutagenesis includes the following specific steps and conditions:

[0028] 1. Stable light waves

[0029] In the dark room, place the 15W ultraviolet lamp above the operating table, and place the 20W infrared lamp as the auxiliary infrared light source on both sides of the operating table. Turn on the ultraviolet light source 30 minutes in advance to stabilize the light wave;

[0030] 2. Aseptic induced mutation

[0031] 100mL of Bdellovibrio sp.BDJ01 culture broth of the wild strain Bdellovibrio sp.BDJ01, which has been deposited in the Chinese Type Culture Collection in Wuhan University, Wuhan City, Wuhan, China, with the deposit number CCTCC NO: M 208011, at 4°C and at a speed of 5000×g Centrifuge for 20 min, take the supernatant after centrifugation and centrifuge at 12000×g for 20 min at 4°C, and suspend the precipitate in 10 mL DNB liquid medium. The su...

Example Embodiment

[0050] Example 2

[0051] The application of UV-induced mutations, with the aid of infrared light sources and LiCl enhanced mutagenesis includes the following specific steps and conditions:

[0052] 1. Stable light waves

[0053] In the dark room, place a 20W ultraviolet lamp above the operating table, and place the 25W infrared lamp as an auxiliary infrared light source on both sides of the operating table. Turn on the ultraviolet light source 60 minutes in advance to stabilize the light wave.

[0054] 2. Aseptic induced mutation

[0055] The concentration method of Budovibrus wild strain BDJ01 is the same as in Example 1, and the concentration is 2×10 7 pfu / mL of the concentrated liquid of the wild strain of Budovibrio; under the state of filtering visible light, the above 2×10 7 Add 5mL of pfu / mL concentrated solution to a 9cm sterile petri dish, and then add 1.67mL LiCl solution with a concentration of 1.0% to the petri dish to make the final concentration reach 0.25%. Add a s...

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Abstract

The invention belongs to the technical field of mutation breeding of microorganisms, and particularly relates to application of ultraviolet-induced mutation in the strengthening pyrolysis performance of bdellovibrio bacteria. The application takes the ultraviolet-induced mutation as a main way and is assisted with an infrared light source and LiCl enhanced mutagenesis to perform induced mutation under the slow stirring, the time is between 2 and 8 minutes, and the radiation distance is between 5 and 20 centimeters; and the bdellovibrio bacteria is preserved under dark light and ice bath conditions and is accumulatively mutagenized until the requirement is achieved. The application has a simple method and convenient operation, has remarkable effect on strengthening the pyrolysis performance of the bdellovibrio bacteria, can pyrolyze two salmonella strains which fail to be pyrolyzed originally and a plurality of Gram-positive bacteria (including bacillus aerogenes capsulatus, clostridium difficile, streptococcus suis and enterococcus) proven by the experiment, solve the problems that usually the bdellovibrio bacteria can not pyrolyze the Gram-positive bacteria and the pyrolysis ability is extremely weak even if the bdellovibrio bacteria can pyrolyze the Gram-positive bacteria, and widen the application range of the bdellovibrio bacteria.

Description

technical field [0001] The invention belongs to the technical field of microbial mutation breeding, and in particular relates to the application of ultraviolet-induced mutation in enhancing the lysing performance of Bdellovibrio. Background technique [0002] For microorganisms in nature, it is difficult to obtain excellent strains if they are simply isolated and purified, and the strains are selected, and generally it is difficult to meet the needs of production. Microbial breeding refers to the use of physical, chemical and other factors to induce variation in the genetic characteristics of microorganisms under artificial conditions, and to select excellent strains with special traits that meet the requirements for cultivation, so that microorganisms can be effectively used in industrial production. [0003] The characteristics of Bdellovibrio that can infect and lyse host bacteria make it suitable as a biological decontamination factor for inhibiting or eliminating pathog...

Claims

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Application Information

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IPC IPC(8): C12N15/01C12N13/00
Inventor 蔡俊鹏韩晓宁
Owner SOUTH CHINA UNIV OF TECH
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