46 results about "Bdellovibrio sp." patented technology

The invention discloses application of bdellovibrio bdelloplast in preparing a bacterial agent for preventing and controlling red mouth disease of scophthalmus maximus. The bdellovibrio is (Bdellovibrio sp.) BDM01. The application method for the bacterial agent comprises that: the bacterial agent is put into water body to prevent and control the red mouth disease of scophthalmus maximus to ensure that the final concentration of the bdellovibrio bdelloplast in the water body is 10-10<7>pfu / mL, or a feed is soaked by the bacterial agent for controlling the red mouth disease of scophthalmus maximus for 15 to 45 minutes, and the concentration of the bdellovibrio bdelloplast in the bacterial agent is 10-10<7>pfu / mL. When the bdellovibrio bdelloplast is applied to preparing the bacterial agent for preventing and controlling the red mouth disease of scophthalmus maximus, the bacterial agent can effectively prevent and control the red mouth disease of scophthalmus maximus, has high safety when applied to aquaculture, is environment-friendly, does not cause pathogenic bacteria resistance, and has good application prospect.

The invention discloses a method for improving shelf life of a saltwater-freshwater bdellovibrio sp. microbial preparation. The method comprises the following steps: preparing the bdellovibrio sp. microbial preparation; mixing the bdellovibrio sp. microbial preparation with a protection agent belonging to different kinds and / or with different concentration, so as to obtain a mixed solution; placing the mixed solution at different temperature to perform accelerated testing; sampling regularly, and detecting the live bacteria concentration of the bdellovibrio sp. microbial preparation in the mixed solution; and utilizing Arrhenius equation and recorded data to calculate out the shelf life of the bdellovibrio sp. microbial preparation, and determining the effect of the protection agent by comparing the shelf life. According to the method, by comparing the quality-guaranteeing effect of different protection agents on the bdellovibrio sp. microbial preparation, a protection agent with best quality-guaranteeing effect is screened out, and the shelf life of the bdellovibrio sp. microbial preparation is obviously prolonged; and also the activity of thalline in the bdellovibrio sp. microbial preparation can be effectively kept for a long time. The provided protection agent is wide in raw material selection scope and cheap in price, and is suitable for popularization and usage.

The invention discloses an energy-saving and emission-reducing abalone fry culture method. An abalone fry culture process comprises the following steps of: (1) cultivating algae; (2) cultivating the abalone fry, namely adding the mixed bacterial liquid of bdellovibrio nectophore and leech plastid in the abalone fry culture process until the concentration of the mixed bacteria in water reaches at least 10 pfu / mL, wherein the bdellovibrio strain is bdellovibrio sp. BDFM05 and is collected by China Center for Type Culture Collection; and the collection number is CCTCC NO:M 209172 and collection date is 2009-08-07; (3) changing water, namely changer water every 5 to 30 days after egg distribution and adding the bdellovibrio mixed bacterial liquid once changing water; and (4) harvesting. The bdellovibrio mixed bacterial liquid has a strong effect for eliminating the common bacteria under the culture environment. The bdellovibrio mixed bacterial liquid has no toxic and side effect on the abalones, so the method has the advantages of suitability for large-scale industrial cultivation of the abalones and capacity of increasing abalone culture survival rate.

The invention discloses marine bdellovibrio and application thereof in promoting the formation of bdelloplast under existence of ampicillin, and belongs to the technical field of bdellovibrio. The marine bdellovibrio is (Bdellovibrio sp.) BDN-1, and was preserved into Guangdong Microorganism Center for Culture Collection in Guandong Microorganism Research Institute (No.5 Floor, No.59 Building, No.100 Central Xian Lie Middle Road, Guangzhou City) on November 27th, 2017, and the preservation number is GDMCC NO:60291. The marine bdellovibrio has the advantages that the factors for promoting the formation of the bdelloplast by the bdellovibrio, such as ampicillin or ampicillin and calcium and magnesium ions, are utilized, and the formation of the bdelloplast by the bdellovibrio BND-1 is promoted; the effect of obviously extending the quality warranty period of the bdellovibrio microorganism preparation is verified; the activity of the bacteria in the bdellovibrio microorganism preparationis effectively maintained for a long time; the culture method for promoting the formation of the bdelloplast is simple and feasible, and is suitable for being popularized and applied, and the contribution is expected to be made to the disease and pest controlling in the aquaculture industry.

The invention discloses an abalone breeding method capable of saving energy and reducing emissions, comprising the following steps of: (1) cultivating algae; (2) breeding seedlings: adding bdellovibrio bacteriovorus leech plastid bacterial liquid during breeding so that the concentration of the leech plastid in water body is at least up to 10pfu / mL, wherein the bdellovibrio bacteriovorus strain is Bdellovibrio sp. BDFM05 which is preserved by China Center for Type Culture Collection on August 7th, 2009, with preservation number of CCTCC NO: M 209172; (3) exchanging water: exchanging the water every 5-30 days after egg distribution, and immediately adding the bdellovibrio bacteriovorus leech plastid bacterial liquid after exchanging water; (4) collecting products. The bdellovibrio bacteriovorus leech plastid has strong effect of eliminating common bacteria in cultivating environment. Since the bdellovibrio bacteriovorus leech plastid has no toxic and side effect to the abalone, the bdellovibrio bacteriovorus leech plastid is adaptable for industrial breeding of abalone in large scales and is capable of improving survival rate of the abalone during breeding.

The invention discloses a young abalone culture method, which comprises the following steps of: (1) paving bricks in a culture pond, and injecting seawater; (2) putting young abalone into the pond, and simultaneously beginning aeration; (3) regularly feeding bait, adding Bdellovibro mixed bacterial liquid into culture water body and / or the fed bait, and changing water every 5-30 days, wherein the water changing quantity each time is a span; and (4) when the young abalone grows to reach 1.0-1.6cm, harvesting the young abalone, wherein the Bdellovibro strain is (Bdellovibrio sp.)BDFM05, which has been preserved by China Center for Type Culture Collection on Aug 7, 2009 with the collection number of CCTCC NO:M209172. The young abalone culture method has the advantages that: (1) the young abalone culture method can save energy and reduce emission, and is more environment-friendly; (2) the Bdellovibro mixed bacterial liquid has the advantages of quick response, strong activity, convenient preparation and long sterilizing time; and (3) the young abalone culture method can obviously improve the physique of the young abalone and enhance the immunity.

The invention discloses an energy-saving and emission-reducing abalone fry culture method. An abalone fry culture process comprises the following steps of: (1) cultivating algae; (2) cultivating the abalone fry, namely adding the mixed bacterial liquid of bdellovibrio nectophore and leech plastid in the abalone fry culture process until the concentration of the mixed bacteria in water reaches at least 10 pfu / mL, wherein the bdellovibrio strain is bdellovibrio sp. BDFM05 and is collected by China Center for Type Culture Collection; and the collection number is CCTCC NO:M 209172 and collection date is 2009-08-07; (3) changing water, namely changer water every 5 to 30 days after egg distribution and adding the bdellovibrio mixed bacterial liquid once changing water; and (4) harvesting. The bdellovibrio mixed bacterial liquid has a strong effect for eliminating the common bacteria under the culture environment. The bdellovibrio mixed bacterial liquid has no toxic and side effect on the abalones, so the method has the advantages of suitability for large-scale industrial cultivation of the abalones and capacity of increasing abalone culture survival rate.

The invention discloses a method for controlling the total number of bacteria in fresh milk. Bdellovibrio is utilized for controlling the total number of bacteria, and the Bdellovibrio strain is a Bdellovibrio (Bdellovibrio sp.) BDFM05 and collected in the China Center for Type Culture Collection (CCTCC for short) with the collection number of M209172 on August 7, 2009. The method comprises the following specific steps of: (1) preparing the Bdellovibrio to Bdellovibrio swimming body bacterial liquid; and (2) adding the Bdellovibrio swimming body bacterial liquid prepared in the step (1) to the fresh milk, and standing or stirring for 1 to 8 hours, wherein the concentration of Bdellovibrio swimming body is at least 102 pfu / ml. The invention can effectively control the growth of the bacteria in the fresh milk, and is beneficial to keeping the original flavor and quality of cowmilk, improving rating number of the fresh milk in acquisition and reducing waste of raw milk caused by excessively rapid growth of the bacteria.

The invention discloses an energy-saving and emission reducing method for raising abalone seedlings, which comprises the following steps of: (1) culturing algae; (2) raising the seedlings, namely adding concentrated solution of bdellovibrio swim body in the process of raising the seedlings, so that the concentration of a swim body in a water body at least reaches 10 pfu / mL finally, wherein bdellovibrio is Bdellovibrio sp. BDFM05 and was collected in the China Center for Type Culture Collection in 7th August, 2009 with the collection number of CCTCC NO: M 209172; (3) changing water, namely changing the water every 5 to 30 days after ovum laying, and adding the concentrated solution of bdellovibrio swim body immediately after water refreshing; and (4) harvesting. The bdellovibrio swim body has the strong effect of eliminating common bacteria in culture environment. The bdellovibrio swim body has no toxic or side effect on abalone, so the bdellovibrio swim body is suitable for large-scale factory cultivation of the abalone, and the culturing survival rate of the abalone can be improved.

The invention discloses application of indole in promoting formation of a marine Bdellovibrio sp. BDE-1 bdelloplast, and belongs to the technical field of Bdellovibrio sp. BDE-1. According to the application of the indole in promoting the formation of the marine Bdellovibrio sp. BDE-1 bdelloplast, a factor, namely, the indole, is formed by utilizing a Bdellovibrio sp. BDE-1 promoting bdelloplast,so that the formation of high-density bdelloplast of the Bdellovibrio sp. BDE-1 is promoted; the research discovers that the indole has an obvious promoting effect on the formation of the bdelloplastof the Bdellovibrio sp. BDE-1; the cracking performance of the bdelloplast is not reduced; compared with a control group, the quality guarantee period of a high-density bdelloplast group is obviouslyprolonged; the high-density bdelloplast provided by the invention can inhibit vibrios which can cause human infection and food poisoning and can also inhibit the vibrios which can cause biological diseases in aquaculture, has a very good inhibiting effect on other food-borne pathogenic bacteria, and has wide cracking spectrum; the cracking effect of the bdelloplast is unchanged as compared with that of a BDE-1 mixture; and a bdelloplast promoting culturing method disclosed by the invention is simple and feasible, and can be popularized and used.

The invention discloses a leech vibrio mutant strain with strong lysis performance and wide lysis spectrum and its application. The leechvibrio mutant strain is Bdellovibrio sp.BDE-1F2, which was deposited in the Guangdong Provincial Microbial Culture Collection Center on November 22, 2018, with the deposit number: GDMCC NO: 60491. The present invention utilizes Co 60 Irradiation-induced mutation method to enhance the lysis performance of Hirudovibrio, effectively improve the lytic ability of Hirudovibrio against Gram-negative and positive pathogenic bacteria and / or potential pathogenic bacteria, and broaden the application scope of Hirudovibrio , to better meet the needs of production practice. The lysis performance of the leech vibrio mutant strain of the present invention is tested to confirm that it can lyse four gram-negative bacteria that cannot be lysed by the original, so that the total lysis rate of 28 indicator bacteria reaches 100%, and the lysis of Gram-negative bacteria is enhanced. The lysis ability of positive bacteria provides a method basis for the enhancement of the lysis performance of Hirudovibrio.

The invention discloses application of bdellovibrio bdelloplast in preparing a bacterial agent for preventing and controlling red mouth disease of scophthalmus maximus. The bdellovibrio is (Bdellovibrio sp.) BDM01. The application method for the bacterial agent comprises that: the bacterial agent is put into water body to prevent and control the red mouth disease of scophthalmus maximus to ensurethat the final concentration of the bdellovibrio bdelloplast in the water body is 10-10<7>pfu / mL, or a feed is soaked by the bacterial agent for controlling the red mouth disease of scophthalmus maximus for 15 to 45 minutes, and the concentration of the bdellovibrio bdelloplast in the bacterial agent is 10-10<7>pfu / mL. When the bdellovibrio bdelloplast is applied to preparing the bacterial agent for preventing and controlling the red mouth disease of scophthalmus maximus, the bacterial agent can effectively prevent and control the red mouth disease of scophthalmus maximus, has high safety when applied to aquaculture, is environment-friendly, does not cause pathogenic bacteria resistance, and has good application prospect.

The invention discloses a strain of marine bdellovibrio and the application of promoting the formation of bdellovibrio under ampicillin, and belongs to the technical field of bdellovibrio. The strain is Bdellovibrio sp. BDN‑1, which was preserved on November 27, 2017 at the Guangdong Provincial Microbiological Culture Collection Center, 5th Floor, Building 59, Compound 59, No. 100 Xianlie Middle Road, Guangzhou City , deposit number: GDMCC NO: 60291. The present invention utilizes ampicillin or ampicillin and calcium and magnesium ions to promote the formation of the Bdellovibrio Bdellovibrio Bideloplasts; it has been confirmed that it has the effect of significantly prolonging the shelf life of Bdellovibrio microbial preparations effect; and can effectively maintain the vitality of the bacterium in the Bdellovibrio microbial preparation for a long time. The method for promoting leech plastid cultivation provided by the invention is simple and feasible, is suitable for popularization and use, and is expected to contribute to the prevention and control of diseases in the aquaculture industry.

The invention in particular relates to an application of a bdellovibrio sp. leech plasmid in cultivating marine products such as penaeus monodon, and the like. The invention is realized by adding a bdellovibrio sp. leech plasmid to the water body and the feeds. The invention is realized specifically based on cultivation pond conditions, preparation before stocking, shrimp postlarvae selecting andstocking, feeding management and water environment management in the cultivation period. The invention is characterized by adding a bdellovibrio sp. leech plasmid bacterium solution to the water bodyand ensuring the concentration of the bdellovibrio sp. leech plasmid in the water body to be 101-107pfu / mL, simultaneously soaking the feeds in the bdellovibrio sp. leech plasmid bacterium solution with the concentration of 101-107pfu / mL for 30 minutes and changing water once every 30 days in the cultivation period. The invention provides a method for cultivating organisms. The method has the advantages of environment friendliness, safety, no pollution, obvious effect and capabilities of improving the water quality, promoting growth of penaeus monodon, improving the immunity and improving thesurvival rate, thus having wonderful development prospect. The invention has very important significance in improving the economic benefits and eating safety and guaranteeing human health.

The invention discloses an application of Bdellovibrio nectophore in preparing a bactericide for controlling the red mouth disease of a scophthalmus maximus. Bdellovibrio is Bdellovibrio sp. BDM01. In the application method of the bactericide, the bactericide for controlling the scophthalmus maximus red mouth disease is splashed into a water body to cause the final concentration of the Bdellovibrio nectophore in the water body to be 10-107pfu / mL, or feeds are soaked by the bactericide for controlling the scophthalmus maximus red mouth disease for 15-45min to cause the concentration of the Bdellovibrio nectophore in the bactericide to be 10-107pfu / mL. The application of the Bdellovibrio nectophore in preparing the bactericide for controlling the scophthalmus maximus red mouth disease can effectively control and treat the red mouth disease of the scophthalmus maximus and has high safety, environmental protection and good application prospect and does not generate pathogenic bacteria resistance in the application of aquatic animal culture.