Water-saving method for culturing young abalones
A culture method and water-saving technology are applied in the field of marine shellfish culture to achieve the effects of enhancing immunity, having remarkable effects and promoting the growth of juvenile abalone.
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Embodiment 1
[0036] Preparation of Bdellovibrio Bdeloidoplasts
[0037] The Bdellovibrio sp. (Bdellovibrio sp.) BDFM05 can form a transparent circular phage plaque with a diameter of 2-3 mm when cultured at 28° C. for three days with a double-layer plate method. The Bdellovibrio sp. Fermentation method of hiruplasma Fermentation: Inoculate 0.5mL of 100 mL of conical flask with 100mL of nutrient broth (10g of peptone, 3g of beef extract powder, 5g of sodium chloride, pH 7.4±0.2). 7cfu / mL Vibrio parahaemolyticus (Vibrio parahaemolyticus, purchased from American Type Culture Collection, American Type Culture Collection, number: ATCC 17802), 200rpm, 28 ℃ shaker culture for 18 hours, the culture solution was centrifuged at 4 ℃, 5000rpm for 15min , discard the supernatant. Use 5mL of normal saline to suspend and precipitate the bacteria, and then add it to a liquid medium containing 100mL DNB (dilute nutrient broth) (nutrient broth 0.8g, casein acid hydrolyzate 0.5g, yeast extract 0.1g, sea cry...
Embodiment 2
[0039] Application of Bdellovibrio Bdeloidoplasma Bacteria Liquid in Breeding
[0040] The breeding ponds in the place where the present invention is implemented are built in the breeding greenhouses, and the specification of each pond is 2×2×0.75m, and the water depth is 0.45m. 8 aeration tubes are placed in each pool. Cover the pool with a shading cloth with a shading degree of 60% to avoid excessive light. The light intensity is generally controlled at (50-200) lux.
[0041] The specific water quality conditions of the sea area where the implementation is carried out are as follows: water temperature 15-28 ° C, salinity 27-33 ‰, pH 6.8-7.2, sea water is treated with sand filtration and sedimentation, and before entering the breeding pool, wrap a 200-mesh mesh bag to prevent fine sand from entering the pool. The water quality of the water source meets the requirements of the national standard of the People's Republic of China for fishery water quality (GB11607-89) and the...
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