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Water-saving method for culturing young abalones

A culture method and water-saving technology are applied in the field of marine shellfish culture to achieve the effects of improving the physique of juvenile abalone, improving the physique of juvenile abalone and enhancing immunity

Inactive Publication Date: 2012-08-22
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it has not been reported at home and abroad to use Bdellovibrio bructoria plastids to carry out juvenile abalone culture.

Method used

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  • Water-saving method for culturing young abalones
  • Water-saving method for culturing young abalones
  • Water-saving method for culturing young abalones

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Preparation of Bdellovibrio Bdeloidoplasts

[0037] The Bdellovibrio sp. (Bdellovibrio sp.) BDFM05 can form a transparent circular phage plaque with a diameter of 2-3 mm when cultured at 28° C. for three days with a double-layer plate method. The Bdellovibrio sp. Fermentation method of hiruplasma Fermentation: Inoculate 0.5mL of 100 mL of conical flask with 100mL of nutrient broth (10g of peptone, 3g of beef extract powder, 5g of sodium chloride, pH 7.4±0.2). 7cfu / mL Vibrio parahaemolyticus (Vibrio parahaemolyticus, purchased from American Type Culture Collection, American Type Culture Collection, number: ATCC 17802), 200rpm, 28 ℃ shaker culture for 18 hours, the culture solution was centrifuged at 4 ℃, 5000rpm for 15min , discard the supernatant. Use 5mL of normal saline to suspend and precipitate the bacteria, and then add it to a liquid medium containing 100mL DNB (dilute nutrient broth) (nutrient broth 0.8g, casein acid hydrolyzate 0.5g, yeast extract 0.1g, sea cry...

Embodiment 2

[0039] Application of Bdellovibrio Bdeloidoplasma Bacteria Liquid in Breeding

[0040] The breeding ponds in the place where the present invention is implemented are built in the breeding greenhouses, and the specification of each pond is 2×2×0.75m, and the water depth is 0.45m. 8 aeration tubes are placed in each pool. Cover the pool with a shading cloth with a shading degree of 60% to avoid excessive light. The light intensity is generally controlled at (50-200) lux.

[0041] The specific water quality conditions of the sea area where the implementation is carried out are as follows: water temperature 15-28 ° C, salinity 27-33 ‰, pH 6.8-7.2, sea water is treated with sand filtration and sedimentation, and before entering the breeding pool, wrap a 200-mesh mesh bag to prevent fine sand from entering the pool. The water quality of the water source meets the requirements of the national standard of the People's Republic of China for fishery water quality (GB11607-89) and the...

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Abstract

The invention discloses a water-saving method for culturing young abalones, comprising the following steps: (1) paving bricks in a culture pond and then pouring seawater into the culture pond; (2) putting the young abalones into the culture pond and starting to carry out aeration; (3) regularly feeding bait and adding a bdellovibro plastid bacterial liquid to a culture water body and / or the fed bait; changing water every 5-30 days with the volume of changed water each time as a measuring range; and (4) collecting the young abalones when the young abalones grow to a length of 1.0-1.6cm, wherein the bacterial strain is Bdellovibrio sp. BDFM05, and preserved in China Center for Preserving Type Culture Collection with the preservation number of CCTCC NO: M209172 on 7th, August 2009. The method provided by the invention has the following advantages: (1) saving energy and reducing emission and being more environment-friendly; (2) enjoying more convenient preparation and preservation of leech plastid, good tolerance and longer bactericidal action; and (3) being capable of invigorating health and enhancing immunity of the young abalones.

Description

technical field [0001] The invention relates to the technical field of seawater shellfish culture, in particular to a culture method that improves the survival rate of juvenile abalone culture, saves water and energy, and is environmentally friendly. Background technique [0002] The research on abalone breeding in our country started in the 1970s, and after nearly 30 years of development, it has made great achievements and remarkable economic benefits. In recent years, with the expansion of the scale of artificial breeding of juvenile abalone, technical defects have gradually become prominent. During the breeding process, especially after the juvenile abalone is peeled off, it is easy to cause some juvenile abalone diseases. With the rapid development of the breeding scale, In the breeding work, there is a phenomenon that a large number of juvenile abalones die in varying degrees within a few days after peeling off. The mortality rate is as high as 80%, and the seedling pre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00A23K1/18A23K1/16C12N1/20C12R1/01A01K61/51A23K10/18A23K50/80
CPCY02A40/81
Inventor 蔡俊鹏蒋小平
Owner SOUTH CHINA UNIV OF TECH
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