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Method for cultivating juvenile abalones

A breeding method and technology for juvenile abalone, which are applied in the directions of microorganism-based methods, biochemical equipment and methods, fish farming, etc. to achieve the effects of enhancing immunity, improving the physique of juvenile abalone, and promoting the growth of juvenile abalone.

Inactive Publication Date: 2012-08-22
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method of using Bdellovibrio swimming body to carry out juvenile abalone breeding has not been reported at home and abroad.

Method used

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  • Method for cultivating juvenile abalones
  • Method for cultivating juvenile abalones
  • Method for cultivating juvenile abalones

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Preparation of Bdellovibrio Swimming Body

[0036] The Bdellovibrio strain used was Bdellovibrio BDFM05. Bdellovibrio BDFM05 was preserved in the Chinese Center for Type Culture Collection of Wuhan University on August 7, 2009, with the preservation number CCTCC NO: M209172. BDFM05 is single-celled, oval, with a size of 1.43×0.53 μm, terminal flagella, and the length of the flagella is at least 2 μm; the Bdellovibrio sp. (Bdellovibrio sp.) BDFM05 can form a diameter 2 ~3mm transparent circular phage plaques, the Bdellovibrio swimming body used is fermented by the fermentation method of high-density Bdellovibrio swimming body: in 100mL nutrient broth (peptone 10g, beef extract powder 3g, sodium chloride 5g, pH 7.4±0.2) conical flask inoculated with 0.5mL, the concentration is 10 7 cfu / mL vibrio parahaemolyticus (Vibrio parahaemolyticus, purchased from the American Type Culture Collection, American Type Culture Collection, number: ATCC 17802), 200rpm, 28 ℃ shaker cultur...

Embodiment 2

[0038] Application of Bdellovibrio Swimming Body Bacterial Liquid in Breeding

[0039] The specific water quality conditions of the sea area where the implementation is carried out are: water temperature 15-28°C, salinity 27-33‰, pH 6.8-7.2, inorganic nitrogen 0.2-0.4mg / mL, phosphate 0.01-0.05mg / mL, no oily substances, Seawater is treated with sand filtration and sedimentation, and before entering the breeding pond, a 200-mesh mesh bag is wrapped at the water inlet pipe of the pond to prevent fine sand from entering the pond. The water quality of the water source meets the requirements of the national standard of the People's Republic of China for fishery water quality (GB11607-89) and the requirements of the Ministry of Agriculture's "Water Quality for Pollution-free Food and Marine Aquaculture" (NY5052-2001).

[0040] The breeding ponds in the place where the present invention is implemented are built in the breeding greenhouses, and the specification of each pond is 2×2×0.7...

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Abstract

The invention discloses a method for cultivating juvenile abalones. The method comprises the following steps of: 1. paving bricks in a cultivating pond, and filling sea water; 2. putting juvenile abalones in the pond, and carrying out aeration simultaneously, 3. periodically feeding bait, adding a bdellovibrio telotroch bacterium liquid to the cultivating water and / or the fed bait, and changing water every 5-30 days by one measuring range every time; and 4. harvesting the juvenile abalones when the length of the juvenile abalones is 1.0-1.6cm. In the method, the bdellovibrio stain is Bdellovibrio sp. BDFM05, which is collected by China Center for Type Culture Collection, the collection number is CCTCC NO: M209172, and the collection date is August 7th, 2009. The invention has the advantages of saving energy, reducing emission reducing and being more environmental friendly; the effect of the bdellovibrio telotroch on controlling and eliminating pathogenic bacteria of the juvenile abalones is quick, and the activity is high; and the method can obviously improve the quality of the juvenile abalones and enhance the immunity.

Description

technical field [0001] The invention relates to the technical field of seawater shellfish culture, in particular to a culture method that improves the survival rate of juvenile abalone culture, saves water and energy, and is environmentally friendly. Background technique [0002] The meat of abalone is tender, delicious, and rich in protein. It has always been a good product for aquaculture. Its shell "Shicasia" is a valuable Chinese medicinal material. The research on abalone breeding in our country started in the 1970s, and after nearly 30 years of development, it has made great achievements and remarkable economic benefits. In recent years, with the expansion of the scale of artificial breeding of juvenile abalone, technical defects have gradually become prominent. During the breeding process, especially after the juvenile abalone is stripped, it is easy to cause some juvenile abalone diseases. In 2010, in Liaoning, Shandong and other places Following the outbreak of lar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00A23K1/18A23K1/16C12N1/20C12R1/01A23K10/16A23K50/80
CPCY02A40/81
Inventor 蔡俊鹏蒋小平
Owner SOUTH CHINA UNIV OF TECH
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