Method for cultivating juvenile abalones
A breeding method and technology for juvenile abalone, which are applied in the directions of microorganism-based methods, biochemical equipment and methods, fish farming, etc. to achieve the effects of enhancing immunity, improving the physique of juvenile abalone, and promoting the growth of juvenile abalone.
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Embodiment 1
[0035] Preparation of Bdellovibrio Swimming Body
[0036] The Bdellovibrio strain used was Bdellovibrio BDFM05. Bdellovibrio BDFM05 was preserved in the Chinese Center for Type Culture Collection of Wuhan University on August 7, 2009, with the preservation number CCTCC NO: M209172. BDFM05 is single-celled, oval, with a size of 1.43×0.53 μm, terminal flagella, and the length of the flagella is at least 2 μm; the Bdellovibrio sp. (Bdellovibrio sp.) BDFM05 can form a diameter 2 ~3mm transparent circular phage plaques, the Bdellovibrio swimming body used is fermented by the fermentation method of high-density Bdellovibrio swimming body: in 100mL nutrient broth (peptone 10g, beef extract powder 3g, sodium chloride 5g, pH 7.4±0.2) conical flask inoculated with 0.5mL, the concentration is 10 7 cfu / mL vibrio parahaemolyticus (Vibrio parahaemolyticus, purchased from the American Type Culture Collection, American Type Culture Collection, number: ATCC 17802), 200rpm, 28 ℃ shaker cultur...
Embodiment 2
[0038] Application of Bdellovibrio Swimming Body Bacterial Liquid in Breeding
[0039] The specific water quality conditions of the sea area where the implementation is carried out are: water temperature 15-28°C, salinity 27-33‰, pH 6.8-7.2, inorganic nitrogen 0.2-0.4mg / mL, phosphate 0.01-0.05mg / mL, no oily substances, Seawater is treated with sand filtration and sedimentation, and before entering the breeding pond, a 200-mesh mesh bag is wrapped at the water inlet pipe of the pond to prevent fine sand from entering the pond. The water quality of the water source meets the requirements of the national standard of the People's Republic of China for fishery water quality (GB11607-89) and the requirements of the Ministry of Agriculture's "Water Quality for Pollution-free Food and Marine Aquaculture" (NY5052-2001).
[0040] The breeding ponds in the place where the present invention is implemented are built in the breeding greenhouses, and the specification of each pond is 2×2×0.7...
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