Young abalone culture method
A culture method and culture pond technology, which are applied in microorganism-based methods, biochemical equipment and methods, fish farming and other directions, can solve problems such as unreported breeding methods, and achieve the effects of improving the physique of juvenile abalone and enhancing immunity
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Embodiment 1
[0036] Preparation of mixed bacterial liquid of Bdellovibrio
[0037] Preparation of Bdellovibrio Bdellovrio sp.: The Bdellovibrio sp. (Bdellovibrio sp.) BDFM05 can form transparent circular phage plaques with a diameter of 2 to 3 mm when cultured at 28° C. for three days with a double-layer plate method. The Bdellovibrio sp. The mixed bacterial solution was fermented by the following method: Inoculate 0.5 mL of 100 mL of conical flask with 100 mL of Nutrient Broth (10 g of peptone, 3 g of beef extract powder, 5 g of sodium chloride, pH 7.4±0.2) 7 cfu / mL Vibrio parahaemolyticus (Vibrio parahaemolyticus, purchased from the American Type Culture Collection, American Type Culture Collection, number: ATCC 17802), 200rpm, 28°C shaker culture for 18 hours, the culture solution was centrifuged at 4°C, 5000rpm for 15min , discard the supernatant. After the precipitate was suspended with 5 mL of normal saline, it was added to a liquid medium containing 100 mL of DNB (dilute nutrient b...
Embodiment 2
[0041] 1. The mixed bacterial liquid of Bdellovibrio is applied in cultivation.
[0042] The breeding ponds in the place where the present invention is implemented are built in the breeding greenhouses, and the specification of each pond is 2×2×0.75m, and the water depth is 0.45m. 8 aeration tubes are placed in each pool. Cover the pool with a shading cloth with a shading degree of 60% to avoid excessive light. The light intensity can be adjusted, and the light intensity is generally controlled at (50-200) lux.
[0043] The specific water quality conditions of the sea area where the implementation is carried out are: water temperature 15-28 ° C, salinity 27-33 ‰, pH 6.8-7.2, sea water is treated with sand filtration and sedimentation, and before entering the breeding pool, a 200 Purpose mesh bag to prevent fine sand from entering the pool. The water quality of the water source meets the requirements of the national standard of the People's Republic of China for fishery wate...
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