Young abalone culture method

A breeding method and technology for breeding ponds, which are applied in microorganism-based methods, biochemical equipment and methods, and fish farming, etc., can solve problems such as unreported breeding methods, and achieve the effects of improving juvenile abalone's physique and enhancing immunity.

Inactive Publication Date: 2011-01-26
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the breeding method of juvenile abalone culture with Bdellovibrio mixed bacterial liquid has not been reported at home and abroad

Method used

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  • Young abalone culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Preparation of mixed bacterial liquid of Bdellovibrio

[0037] Preparation of Bdellovibrio Bdellovrio sp.: The Bdellovibrio sp. (Bdellovibrio sp.) BDFM05 can form transparent circular phage plaques with a diameter of 2 to 3 mm when cultured at 28° C. for three days with a double-layer plate method. The Bdellovibrio sp. The mixed bacterial solution was fermented by the following method: Inoculate 0.5 mL of 100 mL of conical flask with 100 mL of Nutrient Broth (10 g of peptone, 3 g of beef extract powder, 5 g of sodium chloride, pH 7.4±0.2) 7 cfu / mL Vibrio parahaemolyticus (Vibrio parahaemolyticus, purchased from the American Type Culture Collection, American Type Culture Collection, number: ATCC 17802), 200rpm, 28°C shaker culture for 18 hours, the culture solution was centrifuged at 4°C, 5000rpm for 15min , discard the supernatant. After the precipitate was suspended with 5 mL of normal saline, it was added to a liquid medium containing 100 mL of DNB (dilute nutrient b...

Embodiment 2

[0041] 1. The mixed bacterial liquid of Bdellovibrio is applied in cultivation.

[0042] The breeding ponds in the place where the present invention is implemented are built in the breeding greenhouses, and the specification of each pond is 2×2×0.75m, and the water depth is 0.45m. 8 aeration tubes are placed in each pool. Cover the pool with a shading cloth with a shading degree of 60% to avoid excessive light. The light intensity can be adjusted, and the light intensity is generally controlled at (50-200) lux.

[0043] The specific water quality conditions of the sea area where the implementation is carried out are: water temperature 15-28 ° C, salinity 27-33 ‰, pH 6.8-7.2, sea water is treated with sand filtration and sedimentation, and before entering the breeding pool, a 200 Purpose mesh bag to prevent fine sand from entering the pool. The water quality of the water source meets the requirements of the national standard of the People's Republic of China for fishery wate...

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Abstract

The invention discloses a young abalone culture method, which comprises the following steps of: (1) paving bricks in a culture pond, and injecting seawater; (2) putting young abalone into the pond, and simultaneously beginning aeration; (3) regularly feeding bait, adding Bdellovibro mixed bacterial liquid into culture water body and / or the fed bait, and changing water every 5-30 days, wherein the water changing quantity each time is a span; and (4) when the young abalone grows to reach 1.0-1.6cm, harvesting the young abalone, wherein the Bdellovibro strain is (Bdellovibrio sp.)BDFM05, which has been preserved by China Center for Type Culture Collection on Aug 7, 2009 with the collection number of CCTCC NO:M209172. The young abalone culture method has the advantages that: (1) the young abalone culture method can save energy and reduce emission, and is more environment-friendly; (2) the Bdellovibro mixed bacterial liquid has the advantages of quick response, strong activity, convenient preparation and long sterilizing time; and (3) the young abalone culture method can obviously improve the physique of the young abalone and enhance the immunity.

Description

technical field [0001] The invention relates to the technical field of seawater shellfish culture, in particular to a culture method that improves the survival rate of juvenile abalone culture, saves water and energy, and is environmentally friendly. Background technique [0002] As one of the eight treasures in the sea, abalone has always been favored by the market. The meat of abalone is tender, delicious, and rich in protein. It has always been a good product for aquaculture. The research on abalone breeding in our country started in the 1970s. After nearly 30 years of development, it has made great achievements and remarkable economic benefits, but there has been no major breakthrough in the breeding mode. In recent years, with the expansion of the scale of artificial breeding of juvenile abalone, technical defects have gradually become prominent. During the breeding process, especially after the juvenile abalone is stripped, it is easy to cause some juvenile abalone dis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00A23K1/18A23K1/16C12N1/20C12R1/01A23K10/18A23K50/80
CPCY02A40/81
Inventor 蔡俊鹏蒋小平
Owner SOUTH CHINA UNIV OF TECH
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