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Application of Bdellovibrio nectophore in preparing bactericide for controlling red mouth disease of scophthalmus maximus

A technology of Bdellovibrio and swimming body, which is applied in the application field of Bdellovibrio bdelloplasts in the preparation of antibacterial agents for preventing and treating turbot red-mouth disease, can solve food safety and other problems, and achieve effective application, good safety, and high application efficiency. promising effect

Inactive Publication Date: 2011-04-13
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the deficiencies of various food safety problems caused by the abuse of antibiotics in fish farming, the invention provides an application of a Bdellovibrio swimming body in the preparation of a bacterial agent for preventing and treating turbot redmouth disease

Method used

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  • Application of Bdellovibrio nectophore in preparing bactericide for controlling red mouth disease of scophthalmus maximus
  • Application of Bdellovibrio nectophore in preparing bactericide for controlling red mouth disease of scophthalmus maximus

Examples

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Embodiment 1

[0021] Example 1 Preparation of bacterial agent

[0022] The Bdellovibrio strain used was Bdellovibrio BDM01. The Bdellovibriosp. BDM01 was deposited in the China Center for Type Culture Collection on April 28, 2008, and the deposit number is CCTCC NO: M 208066; it was negatively stained and observed under an electron microscope: Bacteria BDM01 is a single cell, arc-shaped, with a size of 1.7×1.0μm, terminal flagella, and a flagella length of 3.5μm; it can be cultured in a double-layer plate method at 28℃ for three days to form a transparent circular phages with a diameter of 3-4mm Plaque.

[0023] Inoculate 1mL in an Erlenmeyer flask containing 100mL of normal nutrient broth (NB) medium (10g peptone, 3g beef extract, 5g sodium chloride, 1000mL distilled water, pH7.4). 9 cfu / mL Aeromonas hydrophila (purchased from the Culture Collection Center of Guangdong Institute of Microbiology, number GIM1.172), cultured on a shaker at 200rpm and 28℃ for 18 hours, and the culture solution was...

Embodiment 2

[0025] Select juvenile fish with healthy physique and uniform individual size, with an average weight of 55±2.5g, according to 300 fish / m 2 The breeding density is put into the pond, and the experiment period of each breeding pond is 4 months.

Embodiment 21

[0027] Normal running water (external drainage is adopted, and the amount of water exchanged every day and night is 8 running water amounts), and regular turbot pellet feed (a breeding method produced by the factory) is put into use, and no bacteria is added.

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Abstract

The invention discloses an application of Bdellovibrio nectophore in preparing a bactericide for controlling the red mouth disease of a scophthalmus maximus. Bdellovibrio is Bdellovibrio sp. BDM01. In the application method of the bactericide, the bactericide for controlling the scophthalmus maximus red mouth disease is splashed into a water body to cause the final concentration of the Bdellovibrio nectophore in the water body to be 10-107pfu / mL, or feeds are soaked by the bactericide for controlling the scophthalmus maximus red mouth disease for 15-45min to cause the concentration of the Bdellovibrio nectophore in the bactericide to be 10-107pfu / mL. The application of the Bdellovibrio nectophore in preparing the bactericide for controlling the scophthalmus maximus red mouth disease can effectively control and treat the red mouth disease of the scophthalmus maximus and has high safety, environmental protection and good application prospect and does not generate pathogenic bacteria resistance in the application of aquatic animal culture.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of fungi, and particularly relates to the application of the Bdellovibrio budoplasma in the preparation of a bacterial agent for preventing and controlling turbot red mouth disease. Background technique [0002] Turbot (Scophthalmus maximus) is a rare flounder genus native to Europe, belonging to the order Flatliformes, Flirtaceae, and turbot genus. Mainly distributed in the Atlantic coast, North Sea, Black Sea and Mediterranean area. Because it has the advantages of adapting to low water temperature life, fast growth, delicious meat, easy to accept artificial feed, easy to intensive breeding, etc., it has successively become the main breeding development target in many countries such as Europe, Chile and South America. Since the turbot was introduced into my country in 1992, its aquaculture has rapidly spread from Shandong to northern coastal provinces, becoming a pillar i...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01P1/00A61K35/74A61P31/04
Inventor 蔡俊鹏鲍星
Owner SOUTH CHINA UNIV OF TECH
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