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Energy-saving and emission reducing method for raising abalone seedlings

An energy-saving emission reduction, abalone technology, applied to enhance immunity, to achieve the effect of improving the physique of abalone seedlings, enhancing immunity, and improving the physique of abalone seedlings

Inactive Publication Date: 2012-08-22
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method of using Bdellovibrio swimming body to raise abalone seedlings has not been reported at home and abroad

Method used

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  • Energy-saving and emission reducing method for raising abalone seedlings
  • Energy-saving and emission reducing method for raising abalone seedlings
  • Energy-saving and emission reducing method for raising abalone seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Preparation of Bdellovibrio Swimming Body

[0039] The Bdellovibrio strain used was Bdellovibrio BDFM05. Bdellovibrio BDFM05 was preserved in the Chinese Center for Type Culture Collection of Wuhan University on August 7, 2009, with the preservation number CCTCC NO: M209172. BDFM05 is single-celled, oval, with a size of 1.43×0.53 μm, terminal flagella, and the length of the flagella is at least 2 μm; the Bdellovibrio sp. (Bdellovibrio sp.) BDFM05 can form a diameter 2 ~3mm transparent circular phage plaques, the Bdellovibrio swimming body used is fermented by the fermentation method of high-density Bdellovibrio swimming body: in 100mL nutrient broth (peptone 10g, beef extract powder 3g, sodium chloride 5g, pH 7.4 ± 0.2) conical flask inoculated with 0.5mL10 7cfu / mL Vibrio parahaemolyticus (Vibrio parahaemolyticus, purchased from American Type Culture Collection, American Type Culture Collection, number: ATCC 17802), 200rpm, 28 ℃ shaker culture for 18 hours, the cultur...

Embodiment 2

[0041] 1. Application of Bdellovibrio swimming body in seedling cultivation

[0042] The present invention can be divided into two stages altogether. The first stage is algae cultivation. In the nursery pond, sand-filtered and sediment-treated seawater is injected and aerated. The attachment base is polyethylene corrugated board. The algae cultivation time is 40 days. No Bdellovibrio swimming body is added in this stage; The second stage is seedling cultivation. The content of the present invention is mainly developed at this stage. The seedling cultivation time is 40 days. At this stage, the experimental group immediately adds the Bdellovibrio swimming body after each water change. The Bdellovibrio swimming bodies obtained in Example 1 were injected with different concentrations of Bdellovibrio swimming bodies into the culture water body at different water exchange periods. The environmental conditions of all experimental pools, including temperature, light, pH value, salini...

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Abstract

The invention discloses an energy-saving and emission reducing method for raising abalone seedlings, which comprises the following steps of: (1) culturing algae; (2) raising the seedlings, namely adding concentrated solution of bdellovibrio swim body in the process of raising the seedlings, so that the concentration of a swim body in a water body at least reaches 10 pfu / mL finally, wherein bdellovibrio is Bdellovibrio sp. BDFM05 and was collected in the China Center for Type Culture Collection in 7th August, 2009 with the collection number of CCTCC NO: M 209172; (3) changing water, namely changing the water every 5 to 30 days after ovum laying, and adding the concentrated solution of bdellovibrio swim body immediately after water refreshing; and (4) harvesting. The bdellovibrio swim body has the strong effect of eliminating common bacteria in culture environment. The bdellovibrio swim body has no toxic or side effect on abalone, so the bdellovibrio swim body is suitable for large-scale factory cultivation of the abalone, and the culturing survival rate of the abalone can be improved.

Description

technical field [0001] The invention relates to a method for raising seedlings of aquaculture shellfish, in particular to a method for promoting rapid growth of abalone seedlings, enhancing immunity and improving the survival rate of abalone seedlings. Background technique [0002] Abalone is one of the precious marine shellfish with high nutritional value. Since the 1970s, my country has started artificial abalone farming. After nearly 30 years of development, the abalone farming industry in Liaoning, Shandong, Fujian, Guangdong and other regions has become one of the local pillar industries. Due to the improvement of the degree of globalization and the deterioration of coastal water quality, abalone disease occurs more and more frequently, and it is becoming more and more serious. Abalone disease may occur in many stages of breeding, especially in the process of raising seedlings, there will often be a large-scale drop phenomenon. Many farms have cultivated seedlings for s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00A23K1/18A23K1/16C12N1/20C12R1/01A23K10/18A23K50/80
CPCY02A40/81
Inventor 蔡俊鹏蒋小平
Owner SOUTH CHINA UNIV OF TECH
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