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Energy-saving and emission-reducing abalone fry culture method

An energy saving and emission reduction, abalone technology is applied to enhance immunity, can solve problems such as environmental pollution and large energy consumption, and achieve the effects of improving the physique of abalone seedlings, having good safety and enhancing immunity.

Inactive Publication Date: 2012-09-05
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of raising abalone seedlings, the traditional aquaculture mode is flow-water aquaculture, with uninterrupted flow of water or a few exchanges a day, which causes a lot of energy consumption, and at the same time, because the aquaculture water is directly discharged without any treatment, it causes environmental pollution
Therefore, "green farming" has become the demand of many farmers and has attracted widespread attention in the aquatic industry. At present, the breeding method of abalone seedlings with Bdellovibrio mixed bacteria liquid has not been reported at home and abroad

Method used

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  • Energy-saving and emission-reducing abalone fry culture method
  • Energy-saving and emission-reducing abalone fry culture method
  • Energy-saving and emission-reducing abalone fry culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Preparation of mixed bacterial liquid of Bdellovibrio

[0038]Preparation of Bdellovibrio swimmers: Centrifuge the supernatant containing Bdellovibrio swimmers at 16000rpm for 20min in the preparation method of Bdellovibrio Bdellovibrio swimmers, then add DNB liquid culture medium (Nutrition broth 0.8g, casein acid hydrolyzate 0.5g, yeast extract 0.1g, dissolved in 1000mL distilled water, pH value is 7.2~7.6) suspend Bdellovibrio swimming body, get 10-10 9 Bdellovibrio swimmer concentrate in pfu / mL.

[0039] Preparation of Bdellovibrio Bdellovrio sp.: The Bdellovibrio sp. (Bdellovibrio sp.) BDFM05 can form transparent circular phage plaques with a diameter of 2 to 3 mm when cultured at 28° C. for three days with a double-layer plate method. The Bdellovibrio sp. The mixed bacterial solution is fermented in the following way: Inoculate 0.5mL10 in an Erlenmeyer flask equipped with 100mL nutrient broth (peptone 10g, beef extract powder 3g, sodium chloride 5g, pH 7.4±0.2) ...

Embodiment 2

[0042] 1. Application of Bdellovibrio mixed bacterial solution in seedling cultivation

[0043] The present invention can be divided into two stages altogether. The first stage is algae cultivation. In the nursery pond, sand-filtered and sediment-treated seawater is injected and aerated. The attachment base is polyethylene corrugated board. The light is controlled at 2000-5000lux, the water temperature is controlled at 15-28°C, and the time for algae cultivation For 40 days, this stage does not add the Bdellovibrio mixed bacterial solution; the second stage is seedling cultivation, the content of the present invention is mainly launched at this stage, and after cultivating algae for 40 days, start to cloth abalone fertilized eggs in the seedling pond, and the seedling time is For 40 days, at this stage, the experimental group added Bdellovibrio mixed bacterial solution immediately after each water change. The Bdellovibrio mixed bacterial solution obtained in Example 1 was use...

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Abstract

The invention discloses an energy-saving and emission-reducing abalone fry culture method. An abalone fry culture process comprises the following steps of: (1) cultivating algae; (2) cultivating the abalone fry, namely adding the mixed bacterial liquid of bdellovibrio nectophore and leech plastid in the abalone fry culture process until the concentration of the mixed bacteria in water reaches at least 10 pfu / mL, wherein the bdellovibrio strain is bdellovibrio sp. BDFM05 and is collected by China Center for Type Culture Collection; and the collection number is CCTCC NO:M 209172 and collection date is 2009-08-07; (3) changing water, namely changer water every 5 to 30 days after egg distribution and adding the bdellovibrio mixed bacterial liquid once changing water; and (4) harvesting. The bdellovibrio mixed bacterial liquid has a strong effect for eliminating the common bacteria under the culture environment. The bdellovibrio mixed bacterial liquid has no toxic and side effect on the abalones, so the method has the advantages of suitability for large-scale industrial cultivation of the abalones and capacity of increasing abalone culture survival rate.

Description

technical field [0001] The invention relates to a method for raising seedlings of aquaculture shellfish, in particular to a method for promoting rapid growth of abalone seedlings, enhancing immunity and improving the survival rate of abalone seedlings. Background technique [0002] Abalone is a kind of marine shellfish. In addition to its delicious meat and high nutritional value, abalone’s iridescent shells are peculiar in shape and sparkle. Nutritious foods are rare marine medicines. With the improvement of people's living standards, the demand for abalone in the international market has increased greatly, and the market prospect is broad. Recently, Australia and other countries have successfully cultivated pearls in abalone, which has further improved the economic benefits of the abalone industry. my country is one of the first countries in the world to eat abalone. In the past 30 years, the status quo of abalone farming in my country has developed rapidly and the resu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00A23K1/18A23K1/16C12N1/20C12R1/01
CPCY02A40/81
Inventor 蔡俊鹏蒋小平
Owner SOUTH CHINA UNIV OF TECH
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