The invention belongs to the technical field of medicine, in particular to a preparation method of milk veteh, oleaster, walnut green husk and worm grass-containing health wine and an oxidation resistant pharmacologic activity effect thereof. The health wine comprises the following raw materials by weight percentage 50-70% of hoantchy root, 10-20% of oleaster, 10-20% of walnut green husk and 10-20% of worm grass. The method comprises the following steps of: smashing the milk veteh, the oleaster, the walnut green husk and the worm grass into powder; putting the powder into a distilling tank; adding low wine of 38-45 degree, the weight of which is 200-300 times of that of medicinal materials, into the distilling tank, and dipping for more than 72 hours; adding with royal jelly to be dissolved after filtering; sealing and storing for more than 90 days; and stirring at one time in every 10-20 days to obtain the health wine. Various external oxidation resistant activity tests prove that thehealth wine has stronger removing capability to tested free radical under the condition of a certain concentration, and has better and comprehensive oxidation resistant effect. Being drunk for a longtime, the health wine can prevent, cure or release various diseases such as heart cerebrovascular disease, cancer, diabetes mellitus, presenile dementia, and the like, and has no poison and side effects.