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Preparation method of biological ink material for 3D printing

A bio-ink and 3D printing technology, applied in the field of 3D printing, can solve the problem of rapid curing molding that is difficult to balance cell compatibility, bioactivity and mechanical properties, cell activity, poor mechanical properties of scaffolds and biocompatibility, and rapid curing molding Difficulties and other problems, to achieve good healing effect, good biocompatibility, good mechanical properties

Inactive Publication Date: 2018-03-02
GUANGDONG TAIBAO MEDICAL DEVICE TECH RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the process of soft tissue repair, there are problems such as long healing period and easy scar hyperplasia. Although the current high-end medical dressings such as hydrogel, foam dressing, hydrocolloid, and alginate medical film can reduce the healing period of soft tissue, the effect is limited.
[0005] The widely used biomedical 3D printing technology is synthetic materials including thermoplastic degradable absorbent polyester plastics (PLA, PLGA, PCL and their copolymers), and some natural polymer materials (alginate, fibrin, collagen, Gelatin, chitosan, hyaluronic acid, etc., most of the above-mentioned synthetic materials are 3D printed with organic solvents or high temperature. The printed scaffold structure is stable, but the accuracy is limited, and it cannot be mixed with cells for printing.
Water-soluble natural polymer materials can be printed with cells at the same time, but the cell activity, scaffold mechanical properties and biocompatibility are poor, and rapid curing and molding are difficult
It is difficult for existing materials to take into account cell compatibility, bioactivity and mechanical properties, and rapid curing molding whether used alone or in combination

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A kind of preparation method that can be used for the biological ink material of 3D printing, comprises the steps:

[0029] (1) Preparation of modified sodium alginate

[0030] Weigh 2 parts of sodium alginate in 100 parts of distilled water at 35°C until completely dissolved, then add 15 parts of glycidyl methacrylate, stir for 45 minutes, and adjust the pH of the reaction system to 8.0 with 1% NaOH solution by mass percentage , and then reacted at 4°C for 48 h. During the reaction, 1% NaOH was continuously added to maintain the pH of the reaction system at 8.0. Finally, it was added into a dialysis bag for dialysis for 48 h. The molecular weight cut-off of the dialysis bag was 14000 Da, and freeze-dried , After irradiation and sterilization under 15 KGY, the modified sodium alginate can be obtained. The degree of substitution of the modified sodium alginate prepared by this method is 35%;

[0031] (2) Preparation of bioink materials

[0032] Dissolve 2 parts of mod...

Embodiment 2

[0035] A kind of preparation method that can be used for the biological ink material of 3D printing, comprises the steps:

[0036] (1) Preparation of modified sodium alginate

[0037] Weigh 2 parts of sodium alginate in 100 parts of distilled water at 35°C until completely dissolved, then add 15 parts of glycidyl methacrylate, stir for 45 minutes, and adjust the pH of the reaction system to 8.5 with 1% NaOH solution by mass percentage , and then reacted at 4°C for 48 h. During the reaction, 1% NaOH was continuously added to maintain the pH of the reaction system at 8.5. Finally, it was added to a dialysis bag for dialysis for 48 h. The molecular weight cut-off of the dialysis bag was 14000 Da, and freeze-dried , After irradiation and sterilization under 20 KGY, the modified sodium alginate can be obtained. The degree of substitution of the modified sodium alginate prepared by this method is 50%;

[0038] (2) Preparation of bioink materials

[0039] Dissolve 5 parts of modif...

Embodiment 3

[0042] A kind of preparation method that can be used for the biological ink material of 3D printing, comprises the steps:

[0043] (1) Preparation of modified sodium alginate

[0044] Weigh 2 parts of sodium alginate in 100 parts of distilled water at 50°C until completely dissolved, then add 20 parts of glycidyl methacrylate, stir for 1 hour, and adjust the pH of the reaction system to 9.0 with 1% NaOH solution by mass percentage , and then reacted at 4°C for 48 h. During the reaction, 1% NaOH was continuously added to maintain the pH of the reaction system at 9.0. Finally, it was added into a dialysis bag for dialysis for 48 h. The molecular weight cut-off of the dialysis bag was 14000 Da, and freeze-dried , After irradiation sterilization under 25 KGY conditions, the modified sodium alginate can be obtained. The degree of substitution of the modified sodium alginate prepared by this method is 60%;

[0045] (2) Preparation of bioink materials

[0046] Dissolve 8 parts of ...

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Abstract

The invention discloses a preparation method of a biological ink material for 3D printing. The preparation method mainly comprises preparing modified sodium alginate, and preparing a biological ink material. According to the present invention, the materials used by the preparation method comprise active cells, a cell culture medium, a photoinitiator, modified sodium alginate and antioxidant enzyme; sodium alginate is modified through light curing, such that the modified sodium alginate can retain the original good biocompatibility and the original good hemostasis of the sodium alginate, and can further has double cross-linking property due to the linking of the light-curable group to the molecule chain of the sodium alginate, such that the rapid curing molding can be achieved; the biological material has good biocompatibility with active cells so as to achieve active printing; and the hydrogel formed through the curing molding of the biological ink material has advantages of good mechanical property, healing promoting, bleeding stopping and good biocompatibility.

Description

technical field [0001] The invention belongs to the field of 3D printing, and in particular relates to a preparation method of a bio-ink material which can be used for 3D printing. Background technique [0002] 3D printing technology is a new manufacturing technology that has emerged in recent years. This technology is mainly under the control of a computer, according to the computer-aided design (CAD) model or computer tomography (CT) of the printed object. Accurate 3D stacking, digital molding technology that quickly manufactures 3D objects of specified shape. [0003] So far, most of the products manufactured by 3D printing technology are used for implanting organs in the body, and there are still great challenges for soft tissue repair, among which the development of biomaterials suitable for 3D printing is the key. [0004] In the process of soft tissue repair, there are problems such as long healing period and easy scar hyperplasia. Currently, high-end medical dressin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00A61L24/00A61L24/08C08F251/00C08F220/32B33Y70/00
CPCA61L24/0005A61L24/0015A61L24/0031A61L24/08A61L26/0023A61L26/0057A61L26/0066A61L26/008A61L2300/232A61L2300/30A61L2300/412A61L2300/418A61L2400/04B33Y70/00C08F251/00C08L5/04C08F220/325
Inventor 韦加娜卢亢李婷
Owner GUANGDONG TAIBAO MEDICAL DEVICE TECH RES INST CO LTD
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