Energy-saving and emission reducing method for raising abalone seedlings
A technology for energy saving, emission reduction and abalone, which is applied to promote the rapid growth of abalone seedlings, enhance immunity, and achieve the effects of improving the survival rate, enhancing immunity and improving the physique of abalone seedlings.
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[0037] Example 1
[0038] Preparation of Budovibrus swimming body
[0039] The Bdellovibrio strain used was Bdellovibrio BDFM05. Bdellovibrio BDFM05 was deposited at the China Type Culture Collection of Wuhan University on August 7, 2009, and the deposit number is CCTCC NO: M209172. BDFM05 is single cell, elliptical, 1.43×0.53μm in size, terminal flagella, and flagella length of at least 2μm. The Bdellovibrio sp. BDFM05 can be cultured at 28°C for three days with a double-layer plate method to form a diameter of 2 ~3mm transparent round plaque, the used Budovibrus swimming body is fermented by the fermentation method of high-density Budovibrus swimming body: in 100mL nutrient broth (peptone 10g, beef extract powder 3g, sodium chloride 5g, pH 7.4±0.2) inoculate 0.5mL10 7 cfu / mL Vibrio parahaemolyticus (purchased from American Type Culture Collection, ATCC 17802), cultured on a shaker at 200 rpm and 28°C for 18 hours, and the culture solution was centrifuged at 4°C and 5000 rpm for...
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[0040] Example 2
[0041] 1. Application of Bdellovibrio swimming body in nursery
[0042] The invention can be divided into two stages. The first stage is the cultivation of algae. In the nursery pond is injected seawater with sand filtration and sedimentation treatment and aerated. The attachment base is polyethylene corrugated board. The algae cultivation time is 40 days. In this stage, no Budovibrio swimming body is added; The second stage is seedling raising. The content of the present invention is mainly carried out at this stage. The seedling raising time is 40 days. In this stage, the experimental group immediately adds the Budovibrium swimming body after each water change. The Bdellovibrio swimming bodies obtained in Example 1 were injected with different concentrations of Bdellovibrio swimming bodies into the culture water with different water exchange cycles. The environmental conditions of all experimental pools, including temperature, light, pH, and salinity, are the...
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