Measuring reagent for activated partial thromboplastin time

A technology of thromboplastin time and reagents, applied in biological tests, material testing products, etc., can solve the problems of widely different results, inconsistent monitoring results, affecting the correctness and timely diagnosis of diseases, etc., to achieve strong stability and improve antioxidant effects Ability, uniformity and good suspension effect

Active Publication Date: 2018-06-29
GUANGZHOU WONDFO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Due to the different quality of APTT reagents used in the laboratory, the results of the same patient measured in different hospitals are very different, resulting in inconsistent monitoring results and affecting the correct and timely diagnosis of the disease.

Method used

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  • Measuring reagent for activated partial thromboplastin time
  • Measuring reagent for activated partial thromboplastin time
  • Measuring reagent for activated partial thromboplastin time

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-1

[0038] Weigh 11.9g HEPES and 4.67gNaCl in a 1.5L glass bottle, add deionized water to 500ml, stir and dissolve, adjust the pH to 7.4, add 50ml concentration of 6wt% nano colloidal silicon dioxide and 0.04mg sulfatide, Stir and mix evenly on a magnetic stirrer; add 2g phospholipids (rabbit cephalin or bovine cephalin), stir for 1h, after fully mixing, add 0.1g astaxanthin; 5gBSA, 10g glycine, 2.1gZnCl 2 , 8g mannitol, 5g trehalose, 1gPVP, stir evenly and dilute to 1L. After standing for 2 hours and filtering, the liquid APTT assay reagent (Example 1-1) was obtained.

[0039] Prepare Examples 1-2, 1-3, 1-4, and 1-5 respectively, and change the content of astaxanthin in Example 1-1 to 0.2g, 0.3g, 0.4g, and 0.5g, respectively.

[0040] The formulation composition of Example 1 and Comparative Example 1 is basically the same, the difference is that in Example 1, astaxanthin is added instead of sodium azide and BHT.

[0041] The APTT assay reagent prepared in Example 1 and Comparat...

Embodiment 2-1

[0050] Weigh 11.9g HEPES and 4.67gNaCl in a 1.5L glass bottle, add deionized water to 500ml, stir and dissolve, adjust the pH to 7.4, add 50ml concentration of 6wt% nano colloidal silicon dioxide and 0.04mg sulfatide, Stir and mix evenly on a magnetic stirrer, add 2g of phospholipid esters (rabbit cephalin or bovine cephalin), stir for 1h, after fully mixing, add 0.3g astaxanthin respectively, add 1g proanthocyanidins and 5gBSA into the glass bottle, 10g glycine, 2.1g ZnCl 2 , 8g mannitol, 5g trehalose, 1gPVP, stir evenly and dilute to 1L. After standing for 2 hours and filtering, it becomes the liquid APTT assay reagent.

[0051] Prepare examples 2-2, 2-3, and 2-4 respectively, and change the content of proanthocyanidins in example 2-1 to: 3g, 5g, and 10g, respectively.

[0052] The preparation composition of Example 1 and Example 2 is basically the same, and Example 2 adds proanthocyanidins on the basis of Examples 1-3 to form a mixed antioxidant with astaxanthin.

[0053...

Embodiment 3

[0080] Embodiment 3: Weigh 7.1g HEPES and 2.0gNaCl in a 1.5L glass bottle, add deionized water to 500ml, stir and dissolve, adjust the pH to 7.0, add 50ml concentration of 1.8wt% nano colloidal silicon dioxide and 0.02mg Sulfatide, stir and mix evenly on a magnetic stirrer, add 1.0g phospholipid (rabbit cephalin or bovine cephalin), stir for 1h, after fully mixing, add 0.1g natural astaxanthin (purchased from SIGMA), add 1g proanthocyanidins, add 2gBSA, 5g glycine, 1.5g ZnCl 2 , 5g mannitol, 2g trehalose, 0.5g PVP, stir well and dilute to 1L. After standing for 2 hours and filtering, it becomes the liquid APTT assay reagent.

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Abstract

The invention discloses a measuring reagent for an activated partial thromboplastin time. The measuring reagent is prepared from a buffer solution, an activator, phospholipid, an antioxidant and a stabilizer, wherein a pH (potential of Hydrogen) value is 7.0 to 7.5; the antioxidant is prepared from astaxanthin; the addition amount of the astaxanthin is 0.1g / L to 0.5g / L. The reagent adopts the astaxanthin as the antioxidant to be capable of effectively generating a reaction with a peroxy radical; thus, the peroxidation chain reaction of lipid is terminated, and the stabile performance of the reagent is further promoted.

Description

technical field [0001] The invention relates to the technical field of coagulation reagents, in particular to a reagent for measuring activated partial thromboplastin time. Background technique [0002] Blood coagulation, or coagulation, refers to the process of blood changing from a liquid state to an immobile gel state, and is an important part of physiological hemostasis. The essence of blood coagulation is the process in which soluble fibrinogen in plasma becomes insoluble fibrin. Coagulation tests are of great significance for the diagnosis of diseases in various clinical departments. In addition to the screening and diagnosis of bleeding diseases, they are also used for the inspection and prediction of various thrombotic diseases and prethrombotic states; disseminated intravascular coagulation ( DIC) laboratory diagnosis, medication guidance and prognosis estimation for patients with various anticoagulation therapy. Thrombosis and hemostasis not only involve basic me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/86
CPCG01N33/86
Inventor 郭琳凤王继华
Owner GUANGZHOU WONDFO BIOTECH
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