Preparation method of liquid acidic pectinase with strong stability

A technology of acid pectinase and strong stability, which is applied in the field of preparation of liquid acid pectinase to achieve the effects of enhanced ion exchange capacity, prevention of loss of enzyme activity, and strong adsorption effect

Inactive Publication Date: 2015-03-25
邵素英
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem solved by the present invention is to overcome the defects of the existing liquid acid pectinase, without adding chemical preservatives, without low-temperature treatment, and adopting high-voltage pulse electric field treatment to the fermented crude enzyme liquid and the deployment concentrated solution at normal temperature, and in the deployment Add pectin decomposition products, Chinese herbal medicine extracts, modified dietary fiber and other natural plant raw materials in the process, then sterilize, filter, and aseptically fill to obtain a stable liquid acid pectinase with a long shelf life

Method used

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  • Preparation method of liquid acidic pectinase with strong stability
  • Preparation method of liquid acidic pectinase with strong stability

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Experimental program
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Embodiment 1

[0057] Embodiment 1 raw material preparation

[0058] 1. Preparation of Pectin Decomposition Products

[0059]The preparation method of the decomposed pectin product is as follows: fresh hawthorn fruit, citrus peel, and apple pomace are respectively put into an ultrasonic cleaning machine equipped with 1.2% sodium bicarbonate solution, cleaned at 200W and 40KHz for 8min, drained, and cut into pieces. Divide into pieces or dices of equal specifications, length and width 5-10mm, thickness 3-5mm, mix evenly according to the mass ratio of 6:3:2, and then freeze at -23°C for 20min; crush immediately, the particle size is 0.5mm; Add deionized water with 4 times the amount of crushed material, mix well, adjust the pH value to 2.5 with citric acid, and carry out high-voltage pulse electric field treatment under the conditions of electric field strength 30kV / cm, pulse time 500μs, pulse frequency 250Hz at room temperature, and then normal Boil under pressure for 40 minutes, filter with...

Embodiment 2

[0069] A preparation method of strong stability liquid acid pectinase, comprising the steps of:

[0070] 1) Add 200 mesh diatomite to the acid pectinase fermentation liquid obtained after fermentation, pre-coat diatomite with a 600 mesh filter cloth, filter with a plate and frame filter, and operate at a pressure of 0.18Mpa to obtain fermented crude enzyme liquid;

[0071] The addition amount of described diatomaceous earth is that every 100mL fermented liquid adds 1g;

[0072] 2) The fermented crude enzyme liquid in step 1) is subjected to high-voltage pulse electric field treatment under the conditions of electric field strength 30kV / cm, pulse time 300μs, and pulse frequency 250Hz at room temperature;

[0073] 3) Step 2) The fermented crude enzyme liquid treated by the high-voltage pulsed electric field is first passed through a microfiltration membrane with a pore size of 0.6 μm, the inlet pressure of the microfiltration is 0.11 MPa, the pressure difference is 0.08 MPa, and...

Embodiment 3

[0082] A preparation method of strong stability liquid acid pectinase, comprising the steps of:

[0083] 1) Add 100 mesh diatomite to the acid pectinase fermentation liquid obtained after fermentation, pre-coat diatomite with a 500 mesh filter cloth, filter with a plate and frame filter, and operate at a pressure of 0.14Mpa to obtain fermented crude enzyme liquid;

[0084] The addition amount of described diatomaceous earth is every 100mL fermented liquid adds 0.5g;

[0085] 2) The fermented crude enzyme liquid in step 1) is subjected to high-voltage pulse electric field treatment under the conditions of electric field strength 25kV / cm, pulse time 200μs, and pulse frequency 200Hz at room temperature;

[0086] 3) Step 2) The fermented crude enzyme liquid treated by the high-voltage pulsed electric field is first passed through a microfiltration membrane with a pore size of 0.4 μm, the inlet pressure of the microfiltration is 0.1 MPa, the pressure difference is 0.07 MPa, and the...

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Abstract

The invention discloses a preparation method of liquid acidic pectinase with strong stability, and belongs to the technical field of preparation of enzyme preparations. The preparation method of the liquid acidic pectinase with strong stability does not need addition of a chemical preservative and low-temperature treatment, and the liquid acidic pectinase with strong stability, which is stable and has a relatively long quality guarantee period, can be obtained by fermenting a crude enzyme solution and blending a concentrated solution by adopting a high-voltage pulse electric field at normal temperature, adding natural plant raw materials including a pectin decomposed product, a Chinese medicinal herb extract, modified dietary fibers and the like during blending, and then performing aseptic filtration and sterile filling. Experiments prove that the liquid acidic pectinase with strong stability, prepared by the method disclosed by the invention, has the total number of bacterial colonies of 75-110CFU/mL after being stored for 12 months at room temperature; and the enzyme activity loss rates of the liquid acidic pectinase which is stored for 12 months at 40 DEG C and 0 DEG C are 1.2-1.4% and 0.5-1.1% respectively.

Description

technical field [0001] The invention relates to a preparation method of liquid acid pectinase, in particular to a preparation method of liquid acid pectinase with strong stability. Background technique [0002] Liquid enzyme preparation is a raw material that is easy to use, environmentally friendly and has good biodegradability. Specific catalytic properties and mild reaction conditions have been more and more used and valued in the industry. In terms of use, liquid enzyme preparations have the following advantages: 1) Liquid enzyme preparations are dispersed in the substrate in the form of molecules or particles, with large dispersion, fast absorption, rapid enzymolysis reactions, and high enzyme activity; 2) easy separation Dosage, easy compounding and secondary processing, easy to use; 3) It is beneficial to improve the utilization rate of enzyme preparations, 4) It can be used directly without dissolution and activation; 5) High purity and low impurity content; 6) Shor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/96C12N9/26
CPCC12N9/96C12N9/2408
Inventor 邵素英
Owner 邵素英
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