Application of Bdellovibrio nectophore in preparing bactericide for controlling red mouth disease of scophthalmus maximus

A technology of Bdellovibrio and swimming body, which is applied in the application field of Bdellovibrio bdelloplasty in the preparation of antibacterial agents for preventing and treating turbot red-mouth disease, can solve food safety and other problems, achieve effective application, and do not produce pathogenic bacteria resistance , good safety effect

Inactive Publication Date: 2013-04-10
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the deficiencies of various food safety problems caused by the abuse of antibiotics in fish farming, the invention provides an application of a Bdellovibrio swimming body in the preparation of a bacterial agent for preventing and treating turbot redmouth disease

Method used

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  • Application of Bdellovibrio nectophore in preparing bactericide for controlling red mouth disease of scophthalmus maximus
  • Application of Bdellovibrio nectophore in preparing bactericide for controlling red mouth disease of scophthalmus maximus

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Embodiment 1

[0021] The preparation of embodiment 1 bacterial agent

[0022] The Bdellovibrio strain used was Bdellovibrio BDM01. The Bdellovibrio sp. BDM01 was preserved in the China Center for Type Culture Collection on April 28, 2008, and the preservation number is CCTCC NO: M 208066; after it was negatively stained, its morphology was observed under an electron microscope: Bdellovibrio sp. The bacteria BDM01 is single-celled, arc-shaped, with a size of 1.7×1.0 μm, terminal flagella, and the length of the flagella is 3.5 μm; it can form a transparent circular phagocytosis with a diameter of 3-4 mm when cultured at 28°C for three days by double-layer plate method. plaque.

[0023] Inoculate 1 mL of conical flask with 100 mL of normal nutrient broth (NB) medium (peptone 10 g, beef extract 3 g, sodium chloride 5 g, distilled water 1000 mL, pH 7.4) at a concentration of 10 9 cfu / mL Aeromonas hydrophila (Aeromonas hydrophila, purchased from the Culture Collection Center of the Guangdong Pr...

Embodiment 2

[0025] Select juvenile fish with healthy physique and uniform individual size, with an average body weight of 55±2.5g, according to 300 fish / m 2 The breeding density is put into the pond, and the experimental period of each breeding pond is 4 months.

Embodiment 21

[0027] Normal running water (using external drainage, the amount of water exchanged every day and night is 8 running water), and the conventional turbot pellet feed (factory-produced breeding method) is put in, and no bacterial agent is added.

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Abstract

The invention discloses an application of Bdellovibrio nectophore in preparing a bactericide for controlling the red mouth disease of a scophthalmus maximus. Bdellovibrio is Bdellovibrio sp. BDM01. In the application method of the bactericide, the bactericide for controlling the scophthalmus maximus red mouth disease is splashed into a water body to cause the final concentration of the Bdellovibrio nectophore in the water body to be 10-107pfu / mL, or feeds are soaked by the bactericide for controlling the scophthalmus maximus red mouth disease for 15-45min to cause the concentration of the Bdellovibrio nectophore in the bactericide to be 10-107pfu / mL. The application of the Bdellovibrio nectophore in preparing the bactericide for controlling the scophthalmus maximus red mouth disease can effectively control and treat the red mouth disease of the scophthalmus maximus and has high safety, environmental protection and good application prospect and does not generate pathogenic bacteria resistance in the application of aquatic animal culture.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of a fungus, in particular to the application of the Bdellovibrio bdelloplast in preparing a fungal agent for preventing and treating turbot red-mouth disease. Background technique [0002] Turbot (Scophthalmus maximus) is a rare flounder flounder native to Europe, belonging to the order Pleuriformes, flounder family, genus Turbot. It is mainly distributed along the Atlantic coast, the North Sea, the Black Sea and the Mediterranean Sea. Because it has the advantages of adapting to low water temperature life, fast growth, delicious meat, easy to accept artificial feed, and easy to intensive farming, it has become the main breeding and development target of many countries such as Europe and South America Chile. Since turbot was introduced into my country in 1992, its culture has rapidly radiated from Shandong to the northern coastal provinces, becoming a pillar industry ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/74
Inventor 蔡俊鹏鲍星
Owner SOUTH CHINA UNIV OF TECH
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