63results about How to "Does not cause death" patented technology

The invention discloses a breeding method for improving the mutation frequency of silkworms. The breeding method includes the following steps that first, silkworm larvae at all developmental stages from four-instar third-day larvae to matured silkworms are taken as silkworm larvae to be handled; second, irradiation holes matched with conceptacle portions of the silkworm larvae to be handled in shape and size are manufactured in a baffle plate of the thickness of 1.5-2.5mm, and then the irradiation holes are aligned with the conceptacle portions of the silkworm larvae to be handled; the conceptacle portions of the silkworm larvae to be handled are irradiated by rays for irradiation through the irradiation holes; third, the irradiated larvae and the control larvae which are not irradiated are bred with the same method, and the matured silkworms are made to spin, spin cocoons and undergo eclosion; fourth, the irradiated eclosion larvae are made to mate with eclosion matured silkworms which are not irradiated, and eggs are laid; mutations of the next generation of bred irradiated silkworms are selected.

The invention discloses a dry land soil animal numeral field and a construction method thereof. The dry land soil animal numeral field comprises at least one dry land capable of being used for a long time, an automatic monitoring device and an electric power supply device, and the distance between every two dry lands is more than 200 m; a square sample plot is arranged in the center of each dry field, and a certain distance is reserved between the sample plot and the edge of the dry field so as to avoid the edge effect; permanent landmarks are respectively arranged at four corners of the sample plot according to the geographic position, or fences are arranged at the boundary of the dry farmland where the sample plot is located; the interior of each sample plot is divided into a plurality of square grids by basic units, and the automatic monitoring equipment and the power supply equipment are arranged around the sample plot, underground, earth surface or overhead. According to the invention, the field dry land soil animal numeral sample plot is digitalized; namely, the digitalized, automatic and intelligent sample plot with full space coverage, all-weather time and three-dimensional samples is realized in three dimensions of space, time and soil animal diversity.

The invention relates to an application of antifolic in constructing a mouse model with neural tube defects (NTDs). Specifically, an anti-folic acid metabolism drug is adopted for intervening a pregnant mouse to generate folic acid dysbolism and cause abnormal embryonic development of the mouse, thereby constructing a mouse model with NTDs. The method adopted by the invention has simplicity of operation, good repeatability, high teratogenesis rate and stable effect, and simulates an animal model close to human on the pathogenesis of NTDs by obstructing normal metabolism of folic acid, thereby facilitating further studies on congenital anomalies, especially NTDs.

The invention provides an insulin immunogen, a preparation method thereof, an insulin antibody, and a kit. The insulin immunogen is an insulin crosslinked body, is formed through intramolecular crosslinking and / or intermolecular crosslinking of a plurality of insulin monomers, and contains one or a plurality of crosslinked monomers disclosed in the invention, wherein 1, and 2 are independently selected from A chain or B chain, R1 is used for representing a group disclosed in the invention, and R2 and R3 are blank, or R1 and R3 are independently selected from a group disclosed in the inventionor -S-, R2 is selected from C2-C74 straight chain or branched chain alkylene, C2-C74 straight chain or branched chain alkylene with a part of -CH2- randomly substituted by -0-, -S-, -NH, -COO-, -CO-,arylidene, heteto arylidene, cycloalkylene, or groups disclosed in the invention, or R1 and R3 are independently selected from a group disclosed in the invention or -S-, and R2 is selected from arylidene or substituted arylidene, wherein in the substituted arylidene, the -H on the substituted arylidene is substituted by -OH, halogen elements, or alkane. The insulin immunogen can be used for obtaining high potency insulin antibody.

The invention discloses an internet-based intelligent earthworm breeding system. The system comprises a Bluetooth module, and the Bluetooth module is used for mutually transmitting data between a breeding box terminal and an operation room terminal and issuing an instruction. According to the system, by utilizing the principle that earthworms escape when the survival ambient temperature is lower than 7 DEG C, the earthworms are captured quickly and conveniently without causing the death of the earthworms, the situation that the earthworms do not feed due to strong light by utilizing breeding in a breeding box, a humidity control unit can provide suitable humidity for the growth of the earthworms in hot summer and rainy seasons is avoided, and the situation that the earthworms do not feed or die and are infected by germs since breeding soil is too dry or too wet is avoided; with the assistance of an infrared sensor and a video monitor, whether or not animals such as mice exist in the breeding box can be found, the animals can be timely captured and expelled, and the situation that a large quantity of earthworms are eaten, and the economic loss is caused is avoided.

The invention discloses a method for cultivating parent fishes of Atlantic salmons in complete fresh water, which comprises the steps of: (1) selecting a breeding pond; (2) controlling cultivation conditions, wherein the cultivation water temperature is controlled to be between 4 and 15 DEG C; the cultivation current gradually changes 3 times per hour and the water flow is 0.04 to 0.09 m<3> / s, 0.5 to 0.8 m<3> / s and 1.3 to 2.0 m<3> / s respectively; and the natural lighting is adopted; (3) selecting the parent fishes; (4) selecting feeds, wherein Atlantic salmon breeding feeds of which the publication number of the patent application is CN101156655A is adopted; and (5) cultivating the parent finishes. According to the living characteristics of parent finishes of the Atlantic salmons, the method can stimulate the gonad genesis of the parent fishes and improve the success rate of the mature parent fishes by adopting unique pond design and particular water flow control.

The invention belongs to the technical field of tree nursery stock cultivation, and particularly relates to a method for increasing the transplanting survival rate of xanthoceras sorbifolia bunge bare-root nursery stocks. The method comprises the steps of 1, farming preparation pretreatment of a planting site; 2, lifting of transplanted seedlings, shaping and clearing of root systems, and planting; 3, field management after xanthoceras sorbifolia bunge bare-root nursery stock transplantation. The first step further comprises the steps of selecting the planting site which contains neutral or alkalescent loam with a pH value ranging between 7 and 8.5, wherein the depth of the soil layer is 60-80 cm; preparing the planting site, wherein farmyard manure is applied 3000 kg / mu before farming preparation; preparing soil and ridging. The second step further comprises the steps of conducting seedling lifting manually, wherein the root systems are cut neatly during seedling lifting; shaping and clearing the root systems; planting the nursery stocks. The third step further comprises the steps of water control of forest land; soil loosening and weeding; final management and maintenance, wherein fertilization and irrigation are conducted three to four times and soil loosening and weeding are conducted twice each year since the second to fifth year. By the adoption of the method, the transplanting survival rate of the xanthoceras sorbifolia bunge bare-root nursery stocks is successfully increased to over 85% from below 20% before measures are taken, and technical assurance is provided for improved variety popularization of xanthoceras sorbifolia bunge.