Semiquantitative determination method of concentration of ammonium nitrogen in agricultural biogas fluid

Abstract

The invention relates to a determination method, in particular to a semiquantitative determination method of concentration of ammonium nitrogen in agricultural biogas fluid; the invention aims to provide the semiquantitative determination method of the concentration of the ammonium nitrogen (namely NH4<+>-N) in the agricultural biogas fluid. The determination method comprises the steps: step 1, selection of raw materials: a cylindrical transparent glass tube containing 20ml of deionized water, a dark plastic pipe containing 2.5ml of alkaline sodium hypochlorite solution, a dark plastic pipe containing 2.5ml of phenol solution, a plastic dropper of which the dripping amount is 0.01ml and a colorimetric card; step 2, determination method: sucking up a biogas fluid sample by using the 0.01ml of plastic dropper, dripping one drop of the biogas fluid to the cylindrical transparent glass tube containing 20ml of deionized water, and ensuring that the one drop of the biogas fluid is free of bubbles; then, adding the 2.5ml of alkaline sodium hypochlorite solution and the 2.5ml of phenol solution in the plastic pipes into the cylindrical transparent glass tube, slightly shaking to uniformly mix the added solution and the primary 20ml of deionized water, then standing the mixture for 1 hour, so the color development is completed.

Description

technical field [0001] The invention relates to a determination method, in particular to a semi-quantitative determination method for ammonium nitrogen concentration in agricultural biogas slurry. Background technique [0002] Ecological circular agriculture is an agricultural development model vigorously advocated by the Chinese government. This model takes into account the economic benefits, social benefits and ecological benefits of agricultural development. It is an important measure related to the sustainable development of my country's agriculture and the protection of the ecological environment. Biogas slurry is an auxiliary product of biogas produced by anaerobic fermentation of livestock and poultry breeding or planting waste. It is a high-quality nutrient material, usually in a moderately weak alkaline, and retains 90% of it except for a large loss of carbon. The nutritional content of raw materials, the nitrogen retention rate is as high as more than 90%, and it i...

AI Technical Summary

Problems solved by technology

In the process of applying biogas slurry to farmland (paddy field and dry land), it is difficult for farmers and large planting bases to know the nitrogen concentration in the irrigated biogas slurry, and it is difficult to control the amount of irrigation. The nitrogen concentration in the biogas slurry or other water samples is measured Generally, it needs to be determined by chemical methods in the laboratory, and the procedure is quite cumbersome. For example, after the total nitrogen is digested by alkaline potassium persulfate, the ultraviolet colorimetry is carried out on the ultraviolet spectrophotometer.

If the concentration of the measured sample is too high, it is necessary to perform colorimetry after dilution treatment, and then calculate the total nitrogen content according to the determined concentration standard curve; NH 4 + -N uses the indophenol blue colorimetric method to perform colorimetry on a visible spectrophotometer, and then calculates NH according to the determined concentration standard curve. 4 + -N content, the total nitrogen determination method in the laboratory is better than that of NH 4 + The determination method of -N is cumbersome, and the concentration cannot be distinguished by color