Application of antifolic in constructing mouse model with neural tube defects (NTDs)

A neural tube defect and mouse model technology, applied in the field of applied basic medical research, to achieve high teratogenic rate, wide application range, and high sensitivity

Inactive Publication Date: 2011-11-02
首都儿科研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The antifolate drug of the present invention is used to prepare and construct the neural tube defect mouse model, and no relevant reports have been seen so far

Method used

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  • Application of antifolic in constructing mouse model with neural tube defects (NTDs)
  • Application of antifolic in constructing mouse model with neural tube defects (NTDs)
  • Application of antifolic in constructing mouse model with neural tube defects (NTDs)

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Embodiment Construction

[0031] The following examples are only for those skilled in the art to better understand the present invention, but do not limit the present invention in any way.

[0032] "Example" Construction of Neural Tube Defect Mouse Model

[0033] 1. Experimental animals and materials

[0034] SPF grade adult C57BL / 6J mice, male and female, 7-8 weeks old, weighing 19-20 g. Purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., license number: SCXK (Beijing) 2006-0009. The use of experimental animals follows the Order of the Ministry of Health of the People's Republic of China (No. 55) - Implementation Rules for the Management of Medical Experimental Animals, and the regulations of the Medical Experimental Animal Management Committee of the Capital Institute of Pediatrics. Rats were fed with breeding feed (purchased from Beijing Huafukang Biotechnology Co., Ltd.), and had regular drinking water.

[0035] MTX was purchased from Merck Company, and the concentrati...

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Abstract

The invention relates to an application of antifolic in constructing a mouse model with neural tube defects (NTDs). Specifically, an anti-folic acid metabolism drug is adopted for intervening a pregnant mouse to generate folic acid dysbolism and cause abnormal embryonic development of the mouse, thereby constructing a mouse model with NTDs. The method adopted by the invention has simplicity of operation, good repeatability, high teratogenesis rate and stable effect, and simulates an animal model close to human on the pathogenesis of NTDs by obstructing normal metabolism of folic acid, thereby facilitating further studies on congenital anomalies, especially NTDs.

Description

Technical field: [0001] The invention belongs to the field of applied basic medical research and relates to the application of antifolate drugs in the preparation and construction of neural tube defect mouse models. Background technique: [0002] Neural tube defects (NTDs) are a common and serious birth defect caused by folic acid deficiency, with an average incidence of 0.05% to 0.2% worldwide. In China, the incidence rate of NTDs is much higher than the above ratio, ranging from 0.2% to 0.4%. It is known as a country with a high incidence of neural tube defects in the world. NTDs are the main cause of death of fetuses, newborns and infants, as well as the main factor leading to children's mental retardation and lifelong disability. They not only cause a great burden to the national economy, but also bring pain and misfortune to family life. [0003] Establishing a suitable animal model helps to understand the etiology, pathogenesis and pathological characteristics of huma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K49/00A01K67/027
Inventor 张霆谢秋牛勃向前王明生关涛王秀伟官臻王建华王国亮朱智强
Owner 首都儿科研究所
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