Application of antifolic in constructing mouse model with neural tube defects (NTDs)

Abstract

The invention relates to an application of antifolic in constructing a mouse model with neural tube defects (NTDs). Specifically, an anti-folic acid metabolism drug is adopted for intervening a pregnant mouse to generate folic acid dysbolism and cause abnormal embryonic development of the mouse, thereby constructing a mouse model with NTDs. The method adopted by the invention has simplicity of operation, good repeatability, high teratogenesis rate and stable effect, and simulates an animal model close to human on the pathogenesis of NTDs by obstructing normal metabolism of folic acid, thereby facilitating further studies on congenital anomalies, especially NTDs.

Description

Technical field: [0001] The invention belongs to the field of applied basic medical research and relates to the application of antifolate drugs in the preparation and construction of neural tube defect mouse models. Background technique: [0002] Neural tube defects (NTDs) are a common and serious birth defect caused by folic acid deficiency, with an average incidence of 0.05% to 0.2% worldwide. In China, the incidence rate of NTDs is much higher than the above ratio, ranging from 0.2% to 0.4%. It is known as a country with a high incidence of neural tube defects in the world. NTDs are the main cause of death of fetuses, newborns and infants, as well as the main factor leading to children's mental retardation and lifelong disability. They not only cause a great burden to the national economy, but also bring pain and misfortune to family life. [0003] Establishing a suitable animal model helps to understand the etiology, pathogenesis and pathological characteristics of huma...

AI Technical Summary

Problems solved by technology

The antifolate drug of the present invention is used to prepare and construct the neural tube defect mouse model, and no relevant reports have been seen so far

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