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Saprospira sp. and its application

A technology of spirochetes and corruption, applied in the direction of bacteria, biological water/sewage treatment, biochemical equipment and methods, etc., can solve the problems such as insignificant effect

Inactive Publication Date: 2007-01-17
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to control algal blooms, a lot of manpower and material resources have been invested in various places, but the effect is still not significant

Method used

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  • Saprospira sp. and its application
  • Saprospira sp. and its application
  • Saprospira sp. and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, the separation of putrefaction spirochete (Saprospira sp.) PdY3 CGMCC No.1043 and its bacteriological characteristic

[0021] 1. Isolation of Saprospira sp. PdY3 CGMCC No.1043

[0022] Saprospira sp. PdY3 CGMCC No.1043 comes from the water bloom in Dianchi Lake. Add the gauze filtrate of the water bloom in Dianchi Lake to the purely cultured Anabaena. Mix and spread on solid BG11 medium, after 2-3 days algae-dissolving plaques appear, such as Figure 1A shown. Use a scalpel to remove a single plaque under aseptic conditions, put it on a solid medium prepared by using Anabaena jelly solution, and Saprospirasp. PdY3 CGMCC No.1043 will continue to grow from the plaque to the surrounding Diffusion, take the putrefaction spirulina (Saprospira sp.) PdY3 CGMCC No.1043 at the edge of the diffusion and put it on the solid medium of the new Anabaena jelly solution, repeat several times, and the purified spirulina putrefaction (Saprospira sp.) .) PdY3 CGMCC No.1043...

Embodiment 2

[0033] Embodiment 2, process polluted water with spoilage spirochete (Saprospira sp.) PdY of the present invention CGMCC No.1043

[0034] Get 2 bottles of 60ml pure culture of Anabaena in the logarithmic growth phase, one bottle is used as a contrast, add 1ml of putrefaction spirulina (Saprospira sp.) PdY3 in another bottle of Anabaena algae ( The concentration of PdY3 was 5×10 4 bar / ml), 1 day later, the algae solution added with PdY3 began to turn yellow, and after a week, the algal cells were all disintegrated, and the solution became clear (the results were as follows: Figure 4 shown).

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PUM

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Abstract

A Saprospira sp. PdY3 (CGMCC No.1043) is disclosed. It can dissolve the blue algae, such as Anabaena, so it can be used in conjunction with other alga-dissolving factors, such as Bdellovibrio sp. S-23 (CGMCC No.1045) to improve the water quality of lake.

Description

technical field [0001] The invention relates to a bacterium and its application, in particular to a putrefaction spirochete and its application. Background technique [0002] Saprospira grandis was first discovered by Gross in 1911 when he mistook it for a spirochete. It was not until 1962 that Lewin established a pure culture method to observe and found that it could not swim and did not have an axoneme, so he proposed that it should not belong to the order of Spirochetes, but should belong to the flexobacteria. People have discovered more spirochetes putrefaction successively, and established the separation and purification system, and have a further understanding of its growth and nutritional requirements. [0003] In 1997, Lewin reported that it could prey on bacteria in an adhesive manner. Since 1990, Sakata in Japan has discovered that it can dissolve diatoms (diatoms are the food of pearl oysters in cages), and discussed the mechanism of its dissolution of diatoms. ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 安成才石苗刘新尧邹莉廖永红吴为中温东辉张仲凯
Owner PEKING UNIV
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