Biosynthesis gamma-aminobutyric acid preparation and method for preparing same

A technology of aminobutyric acid and biosynthesis, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc.

Inactive Publication Date: 2008-11-26
新疆优康来生物科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] GABA has been used in medicine for a long time, but its application in food is still based on the latest research results on the biological activity of GABA

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The biosynthetic gamma-aminobutyric acid (GABA) preparation contains: 5% or 10% or 30% or 50% or 60% gamma-aminobutyric acid (GABA) by weight percentage.

[0021] making process:

[0022] 1. Fermentation process

[0023] Streptococcus lactis is inoculated into 250mL fermented seed culture medium, is transplanted to 25L fermented seed culture medium for 16h, promptly inoculum size 1.0% (v / v), is transplanted to 1000L fermented medium after cultivating 16h, promptly inoculum size 2.5% ( v / v), continuously cultivated at 30°C for 48h under the condition of no ventilation and pH control

[0024] 2. Fermentation broth pretreatment

[0025] 1) Centrifugation: After the fermentation and cultivation, stop the fermentation, introduce the fermentation liquid into a high-speed centrifuge, centrifuge with a centrifugal force of more than 10000g, and collect the supernatant.

[0026] 2) Flocculation: Add 250 mg / L chitosan to the supernatant of the previous step at 40° C., stir and...

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Abstract

The invention relates to a biosynthesized gamma-aminobutyric acid preparation. By weight percentage, the product comprises 5 percent to 60 percent of gamma-aminobutyric acid. The preparation method of the biosynthesized gamma-aminobutyric acid preparation includes the following steps: first, streptococcus lactis strains are inoculated to a seed medium for fermentation composed of glucose, corn steep powder, defatted soybean flour and monosodium glutamate, thus forming fermentation broth; the fermentation broth is drained into a high speed centrifuge to form clear liquid centrifugally; the clear liquid is added with 250 mg/L chitosan at the temperature of 40 DEG C, and stirred for flocculation; the flocculated fermentation broth to be filtered enters a plate-and-frame filter, thus obtaining filtered clear liquid; the filtered clear liquid passes a cationic resin exchange bed for ion exchange; when ion exchange resins are saturated, the liquid is eluted by deionized water to elute all the glutamate; ammonia is used for eluting the liquid and extracting the gamma-aminobutyric acid. The method realizes large scale production of functional food factor GABA, so the method adapts to the production and processing of food with various forms and specifications as well as GABA products of nutritional foods.

Description

1. Technical field [0001] The invention relates to a health product or medicine with gamma-aminobutyric acid (GABA) as the main component and a preparation method thereof, which is a biosynthetic gamma-aminobutyric acid preparation and a preparation method thereof. 2. Background technology [0002] γ-Aminobutyric acid (GABA) is a natural non-protein amino acid, which is widely distributed and has a certain content in many plants. In animals, GABA is mainly distributed in nervous tissue, such as the most concentrated distribution in the brain tissue of mammals, the content is 1000 times that of monoamines, and the content in peripheral organs is very small. In animal and plant tissues, the content of naturally occurring GABA is low, for example, the content in bean leaves is only 0.04 μg / g fresh weight, so it is not feasible to directly extract GABA from animals and plants and use it as a food ingredient. [0003] The research and application of GABA in food first began in t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00A61K31/197A61P9/12A61P15/12A61P25/24A61P25/20C12N1/20C12R1/46
Inventor 崔晓俊山衡汪洋王献民
Owner 新疆优康来生物科技开发有限公司
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