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Compound microorganism ecological bacterial manure prepared from feces of livestock and poultry and preparation method thereof

A technology of compound microorganisms and poultry manure, which is applied in the field of preparing compound microbial ecological bacterial fertilizer by using poultry manure, can solve the problems of affecting environmental sanitation, polluting water bodies, polluting air, etc., and achieves improved soil structure, high degree of organic matter degradation, and active strong effect

Active Publication Date: 2016-12-07
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large amount of poultry manure is not fully utilized, causing huge pollution, such as land occupation, soil pollution, water pollution, air pollution, environmental sanitation, etc., which has become a problem for production enterprises such as planting, breeding, and agricultural and sideline product processing, as well as governments at all levels. The problem

Method used

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  • Compound microorganism ecological bacterial manure prepared from feces of livestock and poultry and preparation method thereof
  • Compound microorganism ecological bacterial manure prepared from feces of livestock and poultry and preparation method thereof
  • Compound microorganism ecological bacterial manure prepared from feces of livestock and poultry and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The bacterial strain of the composite microbial bacterial fertilizer of the present embodiment constitutes as follows:

[0039] Bacillus subtilis B. subtilis CZ9, 1.0×10 8 cfu / ml;

[0040] Pseudomonas putida cas8, 5.0×10 8 cfu / ml;

[0041] Bacillus Lateraporus B. Lateraporus HF12, 1.0×10 8 cfu / ml;

[0042] A. chroococcum cas1, 2.0×10 8 cfu / ml;

[0043] Saccharomyces S.roseus CZ3, 1.5×10 8 cfu / ml;

[0044] Lactococcus S.lactis CZ5, 3.0×10 8 cfu / ml.

[0045] The preparation method of the present embodiment is as follows:

[0046] (1) Single-strain fermentation: Bacillus subtilis B.subtilis CZ9, Bacillus lateralis B.LateraporusHF12, A.chroococcum cas1, Pseudomonas putida cas8, Saccharomyces S.roseusCZ3, Six strains of Streptococcus lactis S. lactis CZ5 were separately fermented to obtain fermentation broth.

[0047] The specific fermentation process is as follows:

[0048] Bacillus subtilis B.subtilis CZ9 and Bacillus lateralis B.Lateraporus HF12 adopt LBG mediu...

Embodiment 2

[0072] The bacterial strain of the composite microbial bacterial fertilizer of the present embodiment constitutes as follows:

[0073] Bacillus subtilis B. subtilis CZ9, 2.0×10 8 cfu / ml;

[0074] Pseudomonas putida cas8, 7.0×10 8 cfu / ml;

[0075] Bacillus Lateraporus B. Lateraporus HF12, 1.5×10 8 cfu / ml;

[0076] A. chroococcum cas1, 2.5×10 8 cfu / ml;

[0077] Saccharomyces S.roseus CZ3, 2.0×10 8 cfu / ml;

[0078] Lactococcus S.lactis CZ5, 4.0×10 8 cfu / ml.

[0079] The preparation method of the present embodiment is as follows:

[0080] (1) Single-strain fermentation: Bacillus subtilis B.subtilis CZ9, Bacillus lateralis B.Lateraporus HF12, A.chroococcum cas1, Pseudomonas putida cas8, Saccharomyces S.roseusCZ3, Six strains of Streptococcus lactis S. lactis CZ5 were separately fermented to obtain fermentation broth.

[0081] Bacillus subtilis B.subtilis CZ9 and Bacillus lateralis B.Lateraporus HF12 adopt LBG medium (glucose 50g, peptone 10g, yeast extract 5g, sodium ch...

Embodiment 3

[0104] The bacterial strain of the composite microbial bacterial fertilizer of the present embodiment constitutes as follows:

[0105] Bacillus subtilis B.subtilisCZ9, 3.0×10 8 cfu / ml;

[0106] Pseudomonas putida cas8, 8.0×10 8 cfu / ml;

[0107] Bacillus Lateraporus B. Lateraporus HF12, 2.0×10 8 cfu / ml;

[0108] A. chroococcum cas1, 3.0×10 8 cfu / ml;

[0109] Saccharomyces S.roseus CZ3, 3.0×10 8 cfu / ml;

[0110] Lactococcus S.lactis CZ5, 5.0×10 8 cfu / ml.

[0111] The preparation method of the present embodiment is as follows:

[0112] (1) Single-strain fermentation: Bacillus subtilis B.subtilis CZ9, Bacillus lateralis B.Lateraporus HF12, A.chroococcum cas1, Pseudomonas putida cas8, Saccharomyces S.roseusCZ3, Six strains of Streptococcus lactis S. lactis CZ5 were separately fermented to obtain fermentation broth.

[0113] Bacillus subtilis B.subtilis CZ9 and Bacillus lateralis B.Lateraporus HF12 adopt LBG medium (glucose 50g, peptone 10g, yeast extract 5g, sodium chlo...

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Abstract

The invention discloses compound microorganism ecological bacterial manure prepared from feces of livestock and poultry and a preparation method of the compound microorganism ecological bacterial manure. The method comprises the steps that azotobacter chroococcum and pseudomonas putida separated from soil and pig feces are subjected to nitrogen ion implantation for mutagenesis, the high-activity mutant strains are screened; bacillus subtilis, bacillus laterosporus, azotobacter chroococcum, pseudomonas putida, saccharomycetes and lactic streptococci are independently and respectively fermented, and fermented bacteria liquid is obtained; mixing is carried out according to the volume ratio of 1-3:5-8:1-2:2-3:1.5-3:3-5 to obtain compound bacterial liquid; a fresh culture medium of cane molasses is added into the compound bactericide according to the mass concentration of 2-5%; potassium sorbate is added into the compound bacterial liquid according to the mass concentration of 2-2.5%., and uniform stirring is carried out; 0.4-1 g / L attapulgite is added, uniform stirring is carried out, and an active bactericide is obtained; the active bactericide is placed into a septic tank, dry-wet operation is carried out after feces is decomposed, and the solid part is the compound microorganism ecological bacterial manure.

Description

technical field [0001] The invention relates to a microbial fertilizer, in particular to a method for preparing compound microbial ecological bacterial fertilizer by using poultry manure. Background technique [0002] With the development of my country's livestock and poultry breeding industry and the implementation of the "Vegetable Basket Project", the large-scale and intensive livestock and poultry breeding industry has risen rapidly, and the production of livestock and poultry manure has increased year by year. A large amount of poultry manure is not fully utilized, causing huge pollution, such as land occupation, soil pollution, water pollution, air pollution, environmental sanitation, etc., which has become a problem for production enterprises such as planting, breeding, and agricultural and sideline product processing, as well as governments at all levels. The problem. Therefore, realizing the resource utilization of poultry and livestock manure is one of the importa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F3/00C05F17/00C12N15/01C12N1/20C12R1/065C12R1/40C12R1/125C12R1/07C12R1/645C12R1/46
CPCC05F3/00C05F17/00C12N1/16C12N1/20C12N15/01C12N1/205C12R2001/065C12R2001/40C05F11/08Y02E50/30Y02W30/40Y02A40/20
Inventor 吴丽芳黄胜威高战元潘高志李明浩赵薇薇陈雪蔡冬清吴正岩
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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