Marine animal and human pathogenic bacterial-wound vibrion gene diagnostic reagent kit and detecting method

Abstract

The invention provides a gene diagnosing reagent box and the detecting method of sea marine lives (such as shrimp, fish and so on) and human pathogenic bacterium-vibrio alginolyticus. There agent box and the detecting method are designed using a pair of primer as main body of vibrio alginolyticus gene protected area series design. The invention uses poly enzyme chain reaction (PCR) technology to carry on qualitative detection to the special DNA piece of the sea marine product and human pathogenic bacterium-vibrio, the method is quick, and the singularity is excellent, the sensitivity is high; it can be applied to the bacterium tracing and detecting to the sea marine product in each period, it also can be applied to the clinic detection of human intestinal canal acute contagion, and the environment detection and so on.

Description

technical field

[0001] The invention relates to a diagnostic kit and a detection method for marine aquatic economic animals and human pathogenic bacteria, mainly for the genetic diagnosis of Vibrio vulnificus (Vibrio vulnificus). Background technique

[0002] Vibrio vulnificus (Vibrio vulnificus) is a halophilic bacterium widely distributed in coastal waterways, bays and coastline areas and seafood all over the world. This bacterium is an opportunistic pathogenic bacterium, which can break out and become popular under certain conditions. In recent years, with the deterioration of the breeding environment, there have been more and more reports of seafood disease or mass death and human food poisoning caused by Vibrio vulnificus in coastal areas of my country. It is currently known that Vibrio vulnificus can infect many types of aquatic products such as barramundi, European eel, Japanese eel, tilapia, Chinese prawn, eel, grouper, oyster and wrinkled abalone in Australia; Con...

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