Mandarin fish infectious splenorenal necrosis virogene diagnostic kit and detecting method thereof

A spleen and kidney necrosis virus and gene diagnosis technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of troublesome material preparation, difficulty in popularization, limited application and development, etc., to avoid virus spread and epidemic, The effect of improving scientific management efficiency and high practical value
CN1448518AInactive Publication Date: 2003-10-15SUN YAT SEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SUN YAT SEN UNIV
Publication Date
2003-10-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention is mandarin fish infectious spleen and kidney necrosis virus gene diagnosing kit and its usage. The kit and its usage is designed based on two pairs of primers for the conservation sequence of mandarin fish infectious spleen and kidney necrosis virus gene. By means of PCR technology, the present invention detects qualitatively the specific DNA nucleic acid segment of mandarin fish infectious spleen and kidney necrosis virus fast, simply, specifically and sensitively. The present invention may be used in the tracing detection of different stages of mandarin fish cultivation and environment monitoring.
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Description

technical field

[0001] The invention relates to a diagnostic kit and a detection method for diseases of aquatic economic animals, in particular to a kit and a detection method for gene diagnosis of mandarin fish Infectious Spleen and Kidney Necrosis Virus (ISKNV, Infectious Spleen and Kidney Necrosis Virus). Background technique

[0002] Mandarin fish is a famous freshwater cultured species in my country. With the success of mandarin fish artificial breeding and nursery technology, mandarin fish aquaculture has developed rapidly, and at the same time, disease problems have become increasingly serious. The diseases of mandarin fish mainly include viral diseases, bacterial diseases and parasitic diseases. Among them, viral diseases are prevalent rapidly and have a high incidence rate, and there are no effective control measures at present, which seriously affects the development of mandarin fish aquaculture. In recent years, large-scale explosive epidemics have occurred in f...

Claims

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