Female Premature Ovarian Failure Susceptibility Gene Detection Model and Detection Kit

A detection kit, a technology for premature ovarian failure, applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., to achieve the effect of sensitive and specific detection

Active Publication Date: 2022-08-02
广州知力医学诊断技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, there are no related researches and reports on Chinese female premature ovarian failure susceptibility gene detection models in China at present.

Method used

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  • Female Premature Ovarian Failure Susceptibility Gene Detection Model and Detection Kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1 Materials and methods

[0022] 1.1 Materials

[0023] 1.1.1 Research objects

[0024] (1) Premature ovarian failure case group: 192 patients with premature ovarian failure were sampled from the premature ovarian failure specialist clinic of Xinhua Hospital from 2007 to 2008.

[0025] (2) Normal control group: 192 healthy volunteers from Shanghai Normal University and East China University of Science and Technology during the same period. Inclusion criteria: no history of premature ovarian failure, no ovarian cancer and other ovarian-related diseases among myself and my immediate family members within three generations.

[0026] The above two groups were all sampled from Chinese women and were not related to each other.

[0027] 1.1.2 Main reagents

[0028] 1.1.2.1 DNA extraction reagent

[0029] Genomic DNA extraction was performed using a blood genomic DNA extraction kit purchased from QIAGEN.

[0030] 1.1.2.2 PCR reaction reagents

[0031] (1) TaKaRa Ex TaqHo...

Embodiment 2

[0154] This example illustrates the clinical application of the Chinese female premature ovarian failure susceptibility gene detection model constructed in Example 1. Specifically, 192 Chinese female cases and 192 controls collected from Xinhua Hospital were used as the research objects, and 15 single nucleotide polymorphisms (SNPs) loci of 15 genes were genotyped. The mainstream statistical method for the correlation of multiple SNP loci, that is, multifactor dimensionality reduction (MDR), was used for statistical analysis. Chinese women's premature ovarian failure susceptibility gene detection model composed of 15 SNP loci of LHCGR, FGFR1, GLO1, BMP15, KISS1R, ESR1 and CAT has good detection performance for premature ovarian failure, OR=345.3478, P value<0.0001 , the accuracy is 92.97%, the sensitivity is 97.92%, and the specificity is 88.02%. The establishment of a susceptibility gene detection model for premature ovarian failure in Chinese women is the first in China, wh...

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Abstract

The present application discloses a female premature ovarian failure susceptibility gene detection model and a detection kit. The female premature ovarian failure susceptibility gene detection model of the present application consists of SNP loci of fifteen genes of ELN, SOD2, FSHR, DENND1A, IL17RD, FGF8, KAL1, THADA, LHCGR, FGFR1, GLO1, BMP15, KISS1R, ESR1 and CAT . The present application is the first to establish a genetic testing model especially for Chinese women, which is ethnic-specific. Moreover, the detection reagents and kits developed according to the genetic detection model of the present application can accurately, sensitively and specifically detect premature ovarian failure in Chinese women, and provide a new approach and solution for the detection of premature ovarian failure in Chinese women.

Description

technical field [0001] The present application relates to the field of detection of premature ovarian failure in women, and in particular to a detection model and detection kit for a susceptibility gene of women with premature ovarian failure. Background technique [0002] Premature ovarian failure (POF) refers to ovarian failure due to various causes before the age of 40, resulting in symptoms such as increased follicle-stimulating hormone and decreased estrogen levels, mostly manifested as amenorrhea before the age of 40. Premature ovarian failure is one of the most common gynecological diseases in the world. It is also a common and frequently-occurring disease of the female reproductive system. Its incidence is increasing year by year, causing serious harm to female reproductive health and society. Investigations and studies have shown that the incidence of premature ovarian failure in China is relatively high, which not only causes reproductive disorders such as amenorrh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/118C12Q2600/156
Inventor 霍亮
Owner 广州知力医学诊断技术有限公司
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