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Female premature ovarian insufficiency susceptibility gene detection model and detection kit

A detection kit and ovarian function technology, which is applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of insufficient screening of POI patients, and achieve sensitive and specific detection effects

Active Publication Date: 2020-08-18
王雪峰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The few existing POI pathogenic gene diagnostic kits are far from enough to screen all POI patients, and need to be continuously updated and expanded

Method used

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  • Female premature ovarian insufficiency susceptibility gene detection model and detection kit
  • Female premature ovarian insufficiency susceptibility gene detection model and detection kit
  • Female premature ovarian insufficiency susceptibility gene detection model and detection kit

Examples

Experimental program
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Embodiment 1

[0021] Embodiment 1 The following embodiments are only used to illustrate the present application, and should not be construed as limiting the present application.

[0022] 1 Materials and methods

[0023] 1.1 Materials

[0024] 1.1.1 Research object

[0025] (1) Premature ovarian insufficiency case group: 192 patients with premature ovarian insufficiency were sampled from the Gynecology Clinic of Zhujiang Hospital of Southern Medical University from 2014 to 2019.

[0026] (2) Normal control group: 192 healthy volunteers from Zhujiang Hospital of Southern Medical University during the same period. Inclusion criteria: no history of premature ovarian insufficiency, no ovarian cancer and other ovarian-related diseases among myself and my immediate family members within three generations.

[0027] The above two groups were sampled from Chinese Han women, and they were not related to each other.

[0028] 1.1.2 Main reagents

[0029] 1.1.2.1 DNA extraction reagents

[0030] Ge...

Embodiment 2

[0124] This example illustrates the clinical application of the Chinese female premature ovarian insufficiency susceptibility gene detection model constructed in Example 1. Specifically, 192 Chinese female cases and 192 controls collected by Zhujiang Hospital of Southern Medical University were used as research objects, and 9 single nucleotide polymorphisms (single nucleotide polymorphisms, SNPs) sites of 4 genes were genotyped , using the mainstream statistical method for studying the correlation of multiple SNP sites, that is, multifactor dimensionality reduction (MDR) for statistical analysis, and initially established the SNPs of SCUBE1, GPSM1, CNN2 and PSPH 4 genes The Chinese female premature ovarian insufficiency susceptibility gene detection model composed of loci has good detection efficiency of premature ovarian insufficiency, OR=345.3478, P value<0.0001, accuracy 92.97%, sensitivity 97.92%, specificity Degree 88.02%. It is the first time in China to establish a sus...

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Abstract

The invention discloses a female premature ovarian insufficiency susceptibility gene detection model and a detection kit. The female premature ovarian insufficiency susceptibility gene detection modelprovided by the invention is composed of 9 SNP loci of four genes of SCUBE1, GPSM1, CNN2 and PSPH. According to the invention, a gene detection model especially for Chinese females is established forthe first time, which has race specificity. Moreover, a detection reagent and a kit researched and developed according to the gene detection model disclosed by the invention can be used for accurately, sensitively and specifically detecting the premature ovarian insufficiency of Chinese women; and a new way and scheme are provided for detecting the premature ovarian insufficiency of Chinese females.

Description

technical field [0001] The application relates to the field of detection of female premature ovarian insufficiency, in particular to a detection model and a detection kit for female premature ovarian insufficiency susceptibility genes. Background technique [0002] Premature Ovarian Insufficiency (POI), formerly known as premature ovarian failure, refers to the depletion of ovarian reserve in women before the age of 40, characterized by primary or secondary amenorrhea, accompanied by elevated blood gonadotropins and estrogen levels. Levels are reduced, and a series of low estrogen symptoms appear. At present, the incidence of POI is about 1-3% in the world, and it affects about 5 million women of childbearing age in my country. POI has no clinical manifestations in the occult period, but fertility has begun to decline. Once menstrual disorders appear, fertility is almost lost. In terms of treatment, hormone replacement is currently the main method. Methods such as ovarian ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 王雪峰蔡序子肖扬王艳傅惠娇刘绮文
Owner 王雪峰
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