Detection and application of novel cervical cancer metastasis marker

Abstract

The invention belongs to the field of gene engineering, and discloses detection and an application of a novel cervical cancer metastasis marker. The cervical cancer metastasis marker is a lymphatic metastasis mode LVEM wrapped by tumor-associated macrophages, and is applied to diagnosis for distinguishing metastatic and non-metastatic early cervical cancers. Aiming at defects that an existing method for evaluating preoperative early cervical cancer lymph node metastasis is not high in sensitivity, has limitation on detection of micro-metastatic lymph nodes, is not high in timeliness and economic practicability, has false negative or false positive, is not high in sensitivity and specificity of the existing tumor marker and the like, based on a fact that an expression level of the LVEM canserve as an index for evaluating the lymph node metastasis state of a cervical cancer patient, the LVEM is developed as a marker, the expression level of the LVEM in cervical cancer tissue is detectedthrough a multicolor immunofluorescence technology, and the lymph node metastasis state of the cervical cancer patient can be more sensitively and specifically evaluated before an operation.

Description

technical field [0001] The invention belongs to the field of genetic engineering, in particular to the detection and application of a novel cervical cancer metastasis marker. Background technique [0002] Cervical cancer is the most common malignant tumor of the female reproductive system, and it is also the fourth leading cause of cancer death among women worldwide, seriously endangering the life and health of women around the world. Although the combined prevention and treatment model of human papillomavirus (HPV) vaccine, cervical cytology screening and surgery has effectively improved the prognosis of patients with early cervical cancer, it is difficult to achieve a complete cure for metastatic and recurrent cervical cancer, and it is still a cancer-related disease. major risk factors for death. [0003] Lymph node metastasis (LNM) is the main metastasis mode of cervical cancer and the main risk factor affecting postoperative recurrence and death in patients with early ...

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Problems solved by technology

However, the sensitivity and specificity for evaluating lymph node metastasis of cervical cancer are not high. At present, there is no consensus on the ideal cut-off value of SCC-Ag for predicting the risk of pelvic lymph node metastasis. Therefore, preoperative SCC-Ag prediction of pelvic lymph node metastasis still needs a lot of research. evidence

[0011] Through the above analysis, the problems and defects of the existing technology are: the sensitivity of the existing methods for evaluating the lymph node metastasis of early cervical cancer before operation is not high, the detection of micro-metastatic lymph nodes has limitations, and the timeliness and economic practicability are not high. Both have the disadvantages of false negative or false positive and the sensitivity and specificity of existing tumor markers are not high

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