Concretion mycobacterium nucleic acid screening method based on loop-mediated isothermal amplification technique

Abstract

The invention pertains to the clinical medicine field, disclosing a Mycobacterium tuberculosis DNA screening method which is based on a Loop-mediated isothermal amplification technology. A nucleic acid detecting method which is based on Loop-mediated isothermal amplification technical principle, detects the Mycobacterium tuberculosis in sputum. By adopting specific primers, and utilizing LAMP technology platform to amplify the specific area of target gene, the Mycobacterium tuberculosis in sputum is screened and detected in molecular level with the auxiliary of a series of quality control and internal control detecting system. The invention is characterized by simpleness and convenience, economy, quick speed, sensitivity and specific performance, and has wide application prospect.

Description

Technical field [0001] The invention belongs to the field of clinical medicine and relates to a nucleic acid screening method of Mycobacterium tuberculosis. More specifically, it is a nucleic acid screening method for Mycobacterium tuberculosis based on loop-mediated isothermal amplification technology. Background technique [0002] Tuberculosis is a chronic infectious disease caused by infection with Mycobacterium tuberculosis. In human history, tuberculosis, like smallpox, plague, cholera, and other severe infectious diseases, was once widespread throughout the world. In the 1950s, with the advent of anti-tuberculosis chemotherapeutics, the epidemic of tuberculosis was brought under certain control in developed countries. However, due to various reasons in recent years, the tuberculosis epidemic situation in some countries and regions has risen sharply, seriously endangering human health and becoming a major global public health problem. On April 23, 1993, at the 46th World Hea...

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Problems solved by technology

Although it is the "golden index" for diagnosing pulmonary tuberculosis, its main defect is its low positive rate

(2) Sputum tuberculosis culture, the results are highly reliable, and tuberculosis drug susceptibility test can be done, but it takes 6-8 weeks, and the application is limited. High safety requirements are not conducive to the universal screening of Mycobacterium tuberculosis

(3) Polymerase chain reaction (PCR), which has poor specificity and has the advantage that the sensitivity can reach 98% to 100%. At the same time, it requires PCR amplifiers, gel electrophoresis and other related expensive equipment, which is not conducive to grassroots medical epidemic prevention institutions and on-site rapid determination

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