Female premature ovarian failure susceptibility gene detection model and detection kit

A detection kit and technology for premature ovarian failure, applied in the determination/testing of microorganisms, biochemical equipment and methods, etc.

Active Publication Date: 2021-01-26
广州达康基因技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, there are no related researches and reports on Chinese female prema

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  • Female premature ovarian failure susceptibility gene detection model and detection kit

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Experimental program
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Embodiment 1

[0021] 1 Materials and methods

[0022] 1.1 Materials

[0023] 1.1.1 Research object

[0024] (1) Premature ovarian failure case group: samples were collected from 192 premature ovarian failure outpatients of Xinhua Hospital from 2007 to 2008.

[0025] (2) Normal control group: 192 healthy volunteers from Shanghai Normal University and East China University of Science and Technology during the same period. Inclusion criteria: There is no history of premature ovarian failure, no ovarian cancer and other ovarian-related diseases among myself and my immediate family members within three generations.

[0026] The above two groups were sampled from Chinese females, and they were not related to each other.

[0027] 1.1.2 Main reagents

[0028] 1.1.2.1 DNA extraction reagents

[0029] Genomic DNA was extracted using a blood genomic DNA extraction kit purchased from QIAGEN.

[0030] 1.1.2.2 PCR reagents

[0031] (1) TaKaRa Ex TaqHot Start Version: purchased from Treasure Bioeng...

Embodiment 2

[0154] This example illustrates the clinical application of the Chinese female premature ovarian failure susceptibility gene detection model constructed in Example 1. Specifically, 192 cases of Chinese female cases and 192 controls collected by Xinhua Hospital were used as research objects, and 15 single nucleotide polymorphisms (single nucleotide polymorphisms, SNPs) sites of 15 genes were genotyped. The mainstream statistical method of the correlation between multiple SNP loci, that is, multifactor dimensionality reduction (MDR) for statistical analysis, has initially established a set of ELN, SOD2, FSHR, DENND1A, IL17RD, FGF8, KAL1, THADA, The Chinese female premature ovarian failure susceptibility gene detection model composed of SNP sites of fifteen genes including LHCGR, FGFR1, GLO1, BMP15, KISS1R, ESR1 and CAT has good detection efficiency for premature ovarian failure, OR=345.3478, P value<0.0001 , the accuracy is 92.97%, the sensitivity is 97.92%, and the specificity ...

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Abstract

The application discloses a female premature ovarian failure susceptibility gene detection model and detection kit. The female premature ovarian failure susceptibility gene detection model disclosed by the application consists of SNP loci of fifteen genes including ELN, SOD2, FSHR, DENND1A, IL17RD, FGF8, KAL1, THADA, LHCGR, FGFR1, GLO1, BMP15, KISS1R, ESR1 and CAT. According to the application, the gene detection model especially for Chinese females is initiatively established, and has race specificity. Moreover, a detection reagent and a kit which are researched and developed according to thegene detection model disclosed by the application can be used for carrying out accurate, sensitive and specific detection on the premature ovarian failure of Chinese females; and a new approach and scheme are provided for premature ovarian failure detection of Chinese females.

Description

technical field [0001] The application relates to the field of detection of female premature ovarian failure, in particular to a detection model and a detection kit for female premature ovarian failure susceptibility genes. Background technique [0002] Premature ovarian failure (POF) refers to ovarian failure due to a variety of causes in women before the age of 40, leading to symptoms such as increased levels of follicle-stimulating hormone and decreased levels of estrogen, and most of them manifest as amenorrhea before the age of 40. Premature ovarian failure is one of the most common gynecological diseases in the world today. It is also a common and frequently-occurring disease of the female reproductive system. Its incidence is increasing year by year, causing serious harm to women's reproductive health and society. Research shows that the incidence of premature ovarian failure in China is relatively high, which not only causes amenorrhea, infertility and other reproduc...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/118C12Q2600/156
Inventor 霍亮
Owner 广州达康基因技术有限公司
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