The invention discloses an antibacterial printing paste composition, a method for preparing glazed tiles using the same, and glazed tiles. A kind of bacteriostatic printing paste composition, comprises bacteriostatic agent, also comprises bacteriostatic material bonding dispersant and bacteriostatic material protective agent; Calculated by weight percentage, the raw material component of described bacteriostatic material bonding dispersing agent comprises 5 ~20% carbo resin, 5~15% saponite soil, 20~55% silicon boron frit powder, 15~30% alumina and 5~15% opal; calculated according to weight percentage, the antibacterial material protective agent The raw material components include 20-30% zirconia, 10-20% tin oxide, 3-10% yttrium oxide, 5-15% opal, 10-25% lithium carbonate and 5-15% rutile. The prepared glazed tile has good antibacterial performance, good antibacterial durability and long antibacterial time, which solves the problem of poor antibacterial effect and durability when the traditional antibacterial agent is directly mixed into raw glaze.