Bacillus peoriae and application thereof in prevention and treatment of citrus canker

A technology of Bacillus russica and Bacillus, applied in the field of Bacillus russica and its application in the prevention and treatment of citrus canker, can solve the problems of slow progress, weak research on citrus canker control measures, and lack of innovation.

Active Publication Date: 2021-05-25
GUANGXI UNIV FOR NATITIES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although people have begun to actively prevent and control citrus canker, the research work on the prevention and control of citrus canker is still relatively weak, and the progress is relatively slow. The chemical control that mainly relies on production still uses several common agents, and lacks innovation.

Method used

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  • Bacillus peoriae and application thereof in prevention and treatment of citrus canker
  • Bacillus peoriae and application thereof in prevention and treatment of citrus canker
  • Bacillus peoriae and application thereof in prevention and treatment of citrus canker

Examples

Experimental program
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Effect test

Embodiment Construction

[0060] 1. Screening of strains

[0061] 1.1 Material preparation

[0062] Sample sampling: The soil sampling site is Guangxi Nonggang National Nature Reserve. The surface soil was removed, and soil samples at a depth of 15cm below the surface were collected, stored in a 4°C incubator, and brought back to the laboratory for further processing.

[0063] Separation and purification medium:

[0064] LB liquid medium: tryptone 10.0g, yeast powder 5.0g, sodium chloride 5.0g, distilled water 1000mL.

[0065] LB solid medium: tryptone 10.0g, yeast powder 5.0g, sodium chloride 5.0g, agar powder 15g, distilled water 1000mL.

[0066] PDA medium: 200.0g potato, 20.0g glucose, 20.0g agar, 1000mL distilled water.

[0067] 1.2 Isolation, purification and screening of strains

[0068] 1.2.1 Separation and purification

[0069] Add 2g of soil sample to 18ml of sterile water to prepare soil suspension, shake it on a shaker (180r / min) for 30min, and dilute it to 10% with sterile water. -1 ...

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Abstract

The invention discloses two strains of functional microorganism peoriae bacillus. The preservation numbers of the two strains of functional microorganism peoriae bacillus are respectively GDMCC NO: 61542 and GDMCC NO: 61541, and the names of the two strains of functional microorganism peoriae bacillus are respectively Paenibacillus peoriae GXMD-hs-28 or Paenibacillus peoriae GXMD-hs-35. The strain is simple to screen and easy to culture, and the inventor also establishes a corresponding culture method. Researches show that a fermentation broth of the GXMD-hs-28 or GXMD-hs-35 strain has a good prevention and treatment effect on citrus canker, and the inhibition effect of the GXMD-hs-28 or GXMD-hs-35 strain on the citrus canker is equivalent to that of a conventional chemical pesticide. The discovery of the bacterial strain enriches available microbial resources in China, and the bacterial strain has the advantages of stable antibacterial effect, high efficiency and environmental friendliness, and has a good application prospect in the aspect of preventing and treating citrus canker.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to bacillus pierrea and its application in preventing and treating citrus canker. Background technique [0002] Citrus canker is a bacterial disease caused by Xanthomonas axonopodispv.citri (Xac), which can affect most citrus cultivars. It is a major plant quarantine disease at home and abroad. It occurs widely in citrus planting areas in China, and occurs more severely in Guangdong, Guangxi, Jiangxi, Hunan and Fujian. It mainly damages citrus leaves, branches and fruits, and produces canker lesions. The disease can infect citrus leaves, shoots, and fruit. When the leaves are damaged, yellow or yellow-green needle-sized oil-stained spots appear on the back of the leaves, and then gradually bulge on both sides of the leaves to become nearly round, beige lesions. , corky, rough surface, off-white or gray-brown; then the center of the lesion is sunken and cracked like a crater, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/25A01P1/00A01G13/00A01G17/00C12R1/01
CPCA01N63/25A01G13/00A01G17/005Y02A50/30
Inventor 朱英芝卢杰姜明国黄艺燕万艳芳黄文善
Owner GUANGXI UNIV FOR NATITIES
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