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Bacillus pierree and its application in the prevention and treatment of citrus canker

A technology of Bacillus russica and Bacillus, applied in the field of Bacillus russica and its application in the prevention and treatment of citrus canker, can solve the problems of weak research work on citrus canker control measures, lack of innovation, and slow progress

Active Publication Date: 2022-07-08
GUANGXI UNIV FOR NATITIES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although people have begun to actively prevent and control citrus canker, the research work on the prevention and control of citrus canker is still relatively weak, and the progress is relatively slow. The chemical control that mainly relies on production still uses several common agents, and lacks innovation.

Method used

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  • Bacillus pierree and its application in the prevention and treatment of citrus canker
  • Bacillus pierree and its application in the prevention and treatment of citrus canker
  • Bacillus pierree and its application in the prevention and treatment of citrus canker

Examples

Experimental program
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Effect test

Embodiment Construction

[0060] 1. Screening of strains

[0061] 1.1 Material preparation

[0062] Sample sampling: The soil sampling location is the National Nature Reserve of Nonggang, Guangxi. The topsoil is removed, and the soil samples at a depth of 15 cm below the surface are collected, stored in a 4°C incubator, and brought back to the laboratory for further processing.

[0063] Separation and purification medium:

[0064] LB liquid medium: tryptone 10.0g, yeast powder 5.0g, sodium chloride 5.0g, distilled water 1000mL.

[0065] LB solid medium: tryptone 10.0g, yeast powder 5.0g, sodium chloride 5.0g, agar powder 15g, distilled water 1000mL.

[0066] PDA medium: potato 200.0 g, glucose 20.0 g, agar 20.0 g, distilled water 1000 mL.

[0067] 1.2 Isolation, purification and screening of strains

[0068] 1.2.1 Separation and purification

[0069] Add 2g of soil samples to 18ml of sterile water to prepare a soil suspension, shake it on a shaker (180r / min) for 30min, and dilute it to 10 with ste...

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Abstract

The invention discloses two strains of functional microorganisms, Bacillus pierreae, respectively, the preservation numbers are GDMCC NO: 61542, GDMCC NO: 61541, and the names are Paenibacillus peoriae GXMD-hs-28 or Paenibacillus peoriae GXMD-hs-35. The strain is simple to screen and easy to cultivate, and the inventor has also established a corresponding cultivation method. Studies have shown that the fermentation broth of GXMD‑hs‑28 or GXMD‑hs‑35 has a good control effect on citrus canker. The effect is comparable. The discovery of this strain enriches the available microbial resources in my country, and has the advantages of stable bacteriostatic effect, high efficiency and environmental friendliness, and has a good application prospect in the prevention and treatment of citrus canker.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to Bacillus pierreis and its application in preventing and treating citrus canker. Background technique [0002] Citrus canker is a bacterial disease caused by Xanthomonas axonopodispv.citri (Xac), which can affect most citrus cultivars. It is a major phytosanitary disease at home and abroad. It occurs widely in citrus planting areas in China, and occurs more seriously in Guangdong, Guangxi, Jiangxi, Hunan and Fujian, mainly affecting citrus leaves, branch tips and fruits, resulting in canker lesions. The disease can damage citrus leaves, shoots and fruits. When the leaves are damaged, yellow or yellow-green needle-sized oily spots appear on the back of the leaves, and then gradually bulge on both sides of the leaves, becoming nearly circular, beige lesions, and soon the epidermis of the diseased part is broken, spongy, and prominent. , corky, rough surface, gray-white or g...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/25A01P1/00A01G13/00A01G17/00C12R1/01
CPCA01N63/25A01G13/00A01G17/005Y02A50/30
Inventor 朱英芝卢杰姜明国黄艺燕万艳芳黄文善
Owner GUANGXI UNIV FOR NATITIES
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