Difunctional engineering magnetotactic bacterium, construction method, imaging method realized by difunctional engineering magnetotactic bacterium and application of difunctional engineering magnetotactic bacterium
By constructing a bifunctional engineered magnetotactic bacterium, MSR-1-MagLov, expressing the plasmid pBBR1-MagLov-MamC, the compatibility problem between magnetotactic bacteria and magnetically controlled fluorescent proteins was solved. This enabled active magnetic field-targeted movement and reversible magnetic field fluorescence switching, improving the effectiveness of in vivo imaging and tumor diagnosis and treatment, and providing efficient heavy metal detection capabilities.
Patent Information
- Application Number
- CN202610233385.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-02-27
- Publication Date
- 2026-05-26
AI Technical Summary
In existing technologies, magnetotactic bacteria lack stable compatibility with magnetosensitive fluorescent proteins, making it impossible to achieve active magnetic field-targeted movement and reversible magnetic field fluorescence switching. Furthermore, the expression of exogenous proteins inhibits magnetosome synthesis, resulting in low transformation efficiency and poor genetic stability, thus failing to achieve high signal-to-noise ratio in vivo imaging and tumor-targeted diagnosis and treatment.
Through genetic engineering, a bifunctional engineered magnetotactic bacterium, MSR-1-MagLov, expressing the plasmid pBBR1-MagLov-MamC was constructed. The MagLov-MamC fusion protein was expressed using the recombinant PmamC-MamC-(Gly4Ser)3-MagLov fusion gene fragment. Combined with optimized conjugation transfer and fluorescence flow cytometry sorting methods, the stable inheritance of the exogenous gene and its magnetofluorescence function were ensured.
It achieves stable compatibility between magnetosensitive fluorescent proteins and magnetotactic bacteria, and has the functions of active magnetic field targeted motion and magnetic field controllable fluorescence switching, which improves the imaging signal-to-noise ratio and biocompatibility. It is suitable for in vivo tracing, integrated tumor diagnosis and treatment and environmental sensing, and provides efficient heavy metal ion detection capabilities.