3results about How to "Delays resistance development" patented technology

The application discloses a Chinese pear psylla MIRA-CRISPR / Cas12a detection kit and method, and belongs to the technical field of rapid detection of pest resistance. In view of the target resistance mutation (GluCl gene V267I site) of Chinese pear psylla to abamectin, the application first combines the CRISPR / Cas12a system with the MIRA isothermal amplification technology, and establishes a visual detection system with high specificity and high sensitivity. The kit comprises MIRA amplification primers, specific crRNA, Cas12a protease and a fluorescent reporter probe. During detection, only a single head of pear psylla sample is needed, after rapid DNA extraction, MIRA amplification and CRISPR cutting reaction are carried out under the condition of constant temperature of 37 DEG C, the total time consumption is not more than 1 hour, and the result is directly visually judged by a portable blue light lamp (green fluorescence is displayed for a positive sample). The application is simple in operation, does not need large instruments, and the result is intuitive, so that real-time monitoring of the abamectin resistance of Chinese pear psylla in the field can be realized, and technical support is provided for scientific rotation of drugs and precise prevention and control.

This invention relates to the field of pesticides, specifically to a fungicide composition for foliar spraying, its preparation method, and application scheme. The active ingredients of the fungicide composition include quinoline copper and phosphite A. Phosphite A is selected from at least one of phosphorous acid, alkali metal salts or alkaline earth metal salts of phosphorous acid, alkylphosphonic acids containing C1-C6 groups of phosphorous acid or alkali metal salts of alkylphosphonic acids, alkaline earth metal salts, and aluminum salts. In the fungicide composition, the weight ratio of quinoline copper to phosphite A is 99:1 to 1:99. The fungicide composition of this invention has high activity against bacterial and fungal diseases occurring in various crops, such as canker and downy mildew, and also exhibits significant synergistic effects. It not only improves the antibacterial or bacteriostatic effect but also broadens the control spectrum. Simultaneously, it can reduce the number of applications and alleviate environmental pressure, thereby achieving the effect of prevention-oriented and integrated pest management.

The application provides an ALDH1L1 protein and application thereof, and belongs to the technical field of genetic engineering. The amino acid sequence of the protein is shown as SEQ ID NO:1, and the nucleotide sequence of the coding gene is shown as SEQ ID NO:2. The application also provides application of a reagent for inhibiting ALDH1L1 gene expression or protein activity in preparation of a product for inhibiting growth and development of insects, and a high-throughput screening method based on the protein. Experiments prove that teflubenzuron can specifically bind to ALDH1L1 and inhibit the enzyme activity thereof; the ALDH1L1 gene of Drosophila is knocked out by using a CRISPR / Cas9 system, so that the egg laying amount is reduced, the development period is prolonged, and the pupation rate and eclosion rate are significantly reduced. The application firstly determines that ALDH1L1 is a direct action target of benzoylurea insecticides, and can be used for screening of new insecticides, monitoring of pest resistance and creation of green pesticides.