Infectious spleen and kidney necrosis virus vaccine and preparation method and application thereof

A virus vaccine and infectious technology, which are applied in the field of infectious spleen and kidney disease virus vaccine and preparation thereof, can solve the problems of economic loss in aquaculture industry, outbreaks of infectious diseases, etc., and achieve conditions conducive to large-scale promotion and growth. Good, not easy to fail effect

Active Publication Date: 2009-09-16
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the increase of breeding density and the deterioration of the breeding ecological environment, outbreaks of infectious diseases often occur, causing huge economic losses to the aquaculture industry.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Preparation of Infectious Spleen-kidney Necrosis Virus Vaccine

[0030] The biological material of the present embodiment selects the mandarin fish larvae cell line established by the inventor himself, and the relevant content of this cell line has been published in published papers (Dong, C., Weng, S., Shi, X., Xu, X ., Shi, N. & He, J. (2008). DeVelopment of a mandarin fish Siniperca chuatsi fry cell linesuitable for the study of infectious spleen and kidney necrosis Virus (ISKNV). VirusRes 135, 273-81.).

[0031] The preparation method of the present embodiment infectious spleen-kidney necrosis virus vaccine comprises the steps:

[0032] (1) Preparation of cells: Digest and disperse the mandarin fish cell line (MMF-1) with trypsin, add DMEM for complete culture (commercially available) and culture it into sheets at 27°C for inoculation of infectious spleen and kidney necrosis virus (ISKNV) ;

[0033] (2) Virus inoculation and cultivation: After culturing ...

Embodiment 2

[0037] Example 2 Infectious spleen-kidney necrosis virus vaccine is used to prevent ISKNV infection

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PUM

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Abstract

The invention discloses an infectious spleen and kidney necrosis virus vaccine and a preparation method and application thereof. The vaccine is obtained by inoculating the infectious spleen and kidney necrosis virus in fish cells, harvesting and inactivating virus fluid. The preparation method of the vaccine comprises four steps such as preparation of cells, inoculation and cultivation of viruses, collection, inactivation and dilution of viruses and preparation of vaccines. The infectious spleen and kidney necrosis virus vaccine of the invention can be used for preventing infectious spleen and kidney necrosis virus infection of fresh-water and sea fishes, has protective action up to 100%, contains no components which are harmful to humans and can be safely eaten by vaccinated fishes; in addition, the vaccine has no influence on the growth of fishes and has immunity lasting over half a year. The preparation method of the vaccine is simple and the used reagents are low in cost, therefore, the vaccine is favorable for large-scale promotion.

Description

technical field [0001] The invention belongs to the field of biogenetic engineering, and in particular relates to an infectious spleen-kidney necrosis virus vaccine and its preparation method and application. Background technique [0002] Since the 1970s, my country's aquaculture industry has made great achievements, setting off several major waves of algae, prawn, fish and shellfish farming, making my country's total aquatic product farming rank first in the world for many years . However, with the increase of breeding density and the deterioration of the breeding ecological environment, outbreaks of infectious diseases often occur, causing huge economic losses to the aquaculture industry. [0003] Swelling cell virus refers to the genus Swollen cell virus of the family Iridoviridae. It is the pathogen of a severe infectious disease that has recently emerged and has a significant harmful effect on fish. Molecular epidemiological studies have found that there are more than 5...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/12A61P31/20
Inventor 何建国董传甫殷志新翁少萍
Owner SUN YAT SEN UNIV
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