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34 results about "Koi herpesvirus" patented technology
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Cyprinid herpesvirus 3. Cyprinid herpesvirus 3 (also CyHV-3, koi herpes virus, or KHV), is a species of virus causing a viral disease that is very contagious to the common carp Cyrpinus carpio. It is most commonly found in ornamental koi, which are often used in outdoor ponds or as feeder stock.
Antiviral complex traditional Chinese medicine and preparation method and application thereof
ActiveCN102861162ACompatibility is reasonableSimple preparation processPowder deliveryAntiviralsDiseaseSide effect
The invention discloses an antiviral complex traditional Chinese medicine and a preparation method and application of the antiviral complex traditional Chinese medicine. The complex traditional Chinese medicine comprises isatis root, rheum officinale, astragalus mongholicus, folium isatidis, houttuynia cordata and polygonum cuspidatum. The antiviral complex traditional Chinese medicine and the preparation method provided by the invention are reasonable in prescription, and simple in preparation technology; as the clinical test shown, the traditional complex traditional Chinese medicine can be applied to preparing medicine for treating or preventing white spot syndrome of cray, hemorragic disease of grass carp, haematopoietic necrosis of crucian, koi herpes virus disease, channel catfish virus disease, iridovirus disease of siniperca chuatsi, and virosis of aquatic livestock; and the antiviral complex traditional Chinese medicine has high efficiency, is safe and has no toxic and side effect, and has a wide clinical application prospect.
Owner:TONGWEI
LAMP detection kit for Koi herpesvirus and detection method
InactiveCN103060475AEfficient detectionStrong specificityMicrobiological testing/measurementMicroorganism based processesWater baths3-deoxyribose
Owner:YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Constructing method and application of koi brain cell line
ActiveCN105087491AGood growthSensitiveMicroorganism based processesNervous system cellsElectron microscopeHistiocyte
The invention discloses a constructing method and application of a koi brain cell line. The constructing method includes: placing koi brain tissue in digestive liquid A containing collagenase and trypsin for digestion; collecting cells; adding into digestive liquid b containing EDTA-4Na for digestion; performing primary culture and secondary culture after the cells which are digested are collected to obtain the koi brain cell line. Aiming at features of koi brain tissue cells, a digestion method combining collagenase with trypsin is adopted to separate tissue cells for primary culture, the koi brain cell line is constructed successfully and continuously transferred to more than 100 generations already, and a lot of koi brain source cells can be provided; the cells can maintain good growing state, so that the cells can be freeze-stored. The koi brain cell line is sensitive to koi herpes viruses with cytopathy occurring after being exposed to the viruses, and proliferated virus particles can be found through electron microscope observation. And the koi brain cell line can be directly applied to pathogenic characteristic study and vaccine development.
Owner:PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
Koi herpesvirus RT-LAMP detection primer group, kit and detection method thereof
InactiveCN104673934AStrong specificityLow probability of primer-dimerMicrobiological testing/measurementMicroorganism based processesBioinformaticsPolymerase
The invention discloses a koi herpesvirus RT-LAMP (reverse transcription loop-mediated isothermal amplification) detection primer group, a kit and a detection method thereof. The detection primer group comprises a pair of outer primers, a pair of loop primers and a pair of inner primers; the detection kit comprises a primer group, an RT-LAMP reaction liquid, Bst DNA (deoxyribonucleic acid) polymerase and negative / positive control. The detection method of the detection kit comprises the following steps: extracting DNA of a to-be-detected virus, amplifying the sample DNA template at 63-65 DEG C with six specific primers and the Bst DNA polymerase with strand displacement activity, observing whether an S amplification curve exists through a convenient constant temperature fluorescence detector, and determining whether the to-be-detected sample contains koi herpesvirus (KHV) DNA. The kit has the advantages of high specificity, high sensitivity, rapidity and high efficiency, simplicity and convenience in operation, easily readable result, and so on, and is suitable for being generalized and applied to detection units of different levels and aquaculture users.
Owner:INSPECTION AND QUARANTINE TECHNOLOGY CENTER ZHONGSHAN ENTRY EXIT INSPECTION AND QUARANTINE +1
Recombinant koi herpesvirus (KHV) or cyprinid herpesvirus 3 (CyHV-3) and vaccine for the prevention of disease caused by KHV/CyHV-3 in cyprinus carpio carpio or cyprinus carpio koi
InactiveCN101820905ASsRNA viruses negative-senseViral antigen ingredientsDiseaseCyprinus carpio carpio
The present invention refers to a recombinant koi herpesvirus (KHV) or Cyprinid herpesvirus 3 (CyHV-3), which is immunogenic in fish, preferably in carps, more preferably in Cyprinus carpio, and to a vaccine for preventive and / or therapeutic treatment of a disease caused by koi herpesvirus (KHV) or CyHV-3. The 5 recombinant herpesvirus is used to confer immunity on fish, preferably on carps, more preferably on Cyprinus carpio, against a disease caused by koi herpesvirus (KHV) or Cyprinid herpesvirus 3 (CyHV-3).
Owner:UNIV LIEGE
Genetic markers for discrimination and detection of viruses causing infectious aquatic organism diseases, and method of discriminating and detecting the viruses using the same
The present invention relates to genetic markers for discrimination and detection of viruses causing infectious aquatic organism diseases, and a method of discriminating and detecting the viruses using the same, and more particularly to a method for discriminating or detecting viruses causing infectious aquatic organism diseases, the method comprising: selecting and amplifying a DNA nucleotide sequence encoding a gene specific for viral hemorrhagic septicemia virus (VHSV), red sea bream iridovirus (RSIV) or infectious spleen and kidney necrosis virus (ISKNV), which is a virus causing red sea bream iridovirus disease, or Koi herpesvirus (KHV); hybridizing a peptide nucleic acid (PNA) that specifically recognizes the amplification product; controlling the temperature of the hybridization product to obtain a temperature-dependent melting curve; and discriminating the viral type or detecting whether or not fish would be infected with the viral type by analyzing the obtained melting curve to determine a melting temperature.
Owner:REPUBLIC OF KOREA (NAT FISHERIES RES & DEV INST)
Koi herpesvirus ORF25 nucleic acid vaccine
InactiveCN102973951AEasy to produceWill not spreadGenetic material ingredientsVirus peptidesAgricultural scienceImmunogenicity
The invention discloses a koi herpesvirus ORF25 nucleic acid vaccine, which has good immunogenicity. According to the koi herpesvirus ORF25 nucleic acid vaccine, ORF25 gene is cloned to an eukaryotic expression vector pIRES-neo to obtain recombined plasmid. The koi herpesvirus ORF25 nucleic acid vaccine has effective prevention effect to koi herpes.
Owner:JILIN AGRICULTURAL UNIV
KHV (koi herpes virus) CPA (cross priming amplification) detection primer and application
ActiveCN106148565AEfficient detectionStrong specificityMicrobiological testing/measurementMicroorganism based processesWater bathsNucleic acid detection
The invention discloses a KHV (koi herpes virus) CPA (cross priming amplification) detection primer and an application. A KHV CPA detection kit comprises following ingredients: 10*ThermoPol Reaction Buffer, Bst DNA polymerase, dNTPs, cross primers CPF and CPR, detection primers DF and DR, stripping primers DPF and DPR, MgSO4, Betaine and a nucleic acid test strip. The KHV CPA detection primer has the characteristics of simplicity, convenience, rapidness and high specificity and sensitivity and is objective and direct in determination of results, low in cost, convenient to use and very safe to people and the environment. The KHV CPA detection primer not only can be used in a special laboratory, but also can be applied to rapid onsite detection in the field, only one metal bath or water bath kettle is required, and a KHV in a sample can be accurately detected within 1.5 h.
Owner:YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Biosynthetic gene cluster of pentostatin and arabinofuranosyladenine and application of biosynthetic gene cluster
ActiveCN106701788AImprove dynamic characteristicsIncrease productionMicroorganism based processesOxidoreductasesBiosynthetic genesStreptomyces antibioticus
The invention relates to cloning, sequencing, analysis and functional study of a biosynthetic gene cluster of a natural product pentostatin which is produced from streptomyces antibioticus and is used for treating lymphocytic leukemia and a natural product arabinofuranosyladenine for resisting Koi herpes virus and varicella-zoster virus and application of the biosynthetic gene cluster. Biosynthetic genes of the two compounds are included in the same gene cluster and are independent from each other. The whole gene cluster comprises ten genes, namely three genes related to pentostatin synthesis, five genes related to arabinofuranosyladenine synthesis and two genes related to transportation regulation of the pentostatin and arabinofuranosyladenine. According to genetic manipulations of the previous biosynthetic genes, biosynthesis of the pentostatin or arabinofuranosyladenine can be blocked or improved. The genes provided by the invention and proteins thereof can be used for genetic engineering, protein expression, enzymic catalytic reaction and the like of the compounds, and can be further used for searching and discovering compounds or genes and proteins which can be used for medicines, industry or agriculture.
Owner:WUHAN UNIV
Monoclonal antibody against koi herpesvirus (KHV) and cell strain and application thereof
ActiveCN108866008AStrong specificityBiological material analysisImmunoglobulins against virusesStructural proteinCell strain
The invention discloses a preparation method and application of a monoclonal antibody against koi herpesvirus (KHV). The prepared monoclonal antibody against KHV is secreted by a mouse hybridoma cellstrain of which the preservation number is CCTCC NO: C201887, and the titer of the monoclonal antibody is 1:(1.28 is multiplied by 105); and the hybridoma cell strain has high activity, can recognizethe structural protein of the KHV, and has strong specificity. On the basis, a sandwich ELISA detection method for detecting the KHV is established, and the minimum detection concentration is 3.923ng / mL. The monoclonal antibody is utilized for establishing an indirect sandwich ELISA kit for detecting the KHV, and the kit can be used for large-scale detection of the KHV and has wide application prospects.
Owner:PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
Recombinase-aid amplification (RAA) constant-temperature fluorescence detection method and reagent for Koi herpesvirus (KHV)
InactiveCN110592270AEasy to operateMild conditionsMicrobiological testing/measurementMicroorganism based processesForward primerFluorescence
The invention discloses recombinase-aid amplification (RAA) constant-temperature fluorescence detection method and reagent for Koi herpesvirus (KHV). The RAA constant-temperature fluorescence detection reagent for the KHV comprises a forward primer SEQ ID NO. 1, a reverse primer SEQ ID NO. 2, a specific fluorescent probe SEQ ID NO. 3, a reaction solution, recombinant polymerase and a reference substance. The RAA constant-temperature fluorescence detection reagent for the KHV disclosed by the invention is high in specificity, high in detection sensitivity (up to 100fg / microliter), high in accuracy and reliable; and moreover, the reagent is easy and convenient to operate as well as suitable for field testing. Thus, wide range of application scenarios is ensured.
Owner:HANGZHOU ZHONGCE BIO SCI&TECH CO LTD
CNT (carbon nano tube)-loaded nucleic acid vaccine based on KHV (Koi herpesvirus) ORF149 and application
InactiveCN110548137AImprove immunityImprove the level ofViral antigen ingredientsPharmaceutical delivery mechanismCarbon nanotubePercent survival
The invention discloses a CNT (carbon nano tube)-loaded nucleic acid vaccine based on a KHV (Koi herpesvirus) ORF149 and an application. A single-walled CNT-loaded pcDNA-ORF149 nucleic acid vaccine system is constructed successfully, fish antibody level is improved, the relative percent survival of the system is 81.9%, the immune level is far higher than that of other immune systems, and the system has the application potential in aquaculture and pathogen research.
Owner:PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
Primers and method for fast detecting koi herpes viruses (KHV)
PendingCN109913583AAvoid pollutionHigh sensitivityMicrobiological testing/measurementMicroorganism based processesCyprinid herpesvirus 3Fluorescence
The invention discloses primers and method for fast detecting koi herpesviruses (KHV) and belongs to the field of animal pathogen detection. The primers and method are high in specificity and sensitivity and good in repeatability. According to the technical scheme, the nucleotide sequence of the primers is as shown in SEQ ID No. 1 and SEQ ID No. 2, and the nucleotide sequence of a probe is as shown in SEQ ID No. 3. The detecting method includes the steps of firstly, extracting virus genome DNA from a clinical sample; secondly, using the extracted sample DNA as the template, using the primer group in claim 1 and the probe in claim 2 to prepare an RPA reaction system, and using a TwistAmp exo kit to perform RPA amplification reaction; thirdly, placing the reaction tube after the RPA amplification into a fluorescence detector, monitoring fluorescent signals in real time, determining that the sample contains KHV if an evident fluorescent signal curve exists, and determining that the sampledoes not contain KHV if no fluorescent signal curves exist.
Owner:广州动佰生物科技有限公司 +3
Dual real-time fluorescent quantitative detection method for carp edema virus and koi herpes virus
ActiveCN109897918BHigh sensitivityStrong specificityMicrobiological testing/measurementDNA/RNA fragmentationCarp edema virusConserved sequence
The invention discloses a dual real-time fluorescent quantitative PCR detection method for carp edema virus and koi herpes virus. The method includes the following steps: first, synthesizing primers and TaqMan probes: optimizing the primer sequence according to the CEV P4a gene sequence, The first base of the upstream primer was changed to a degenerate base W, and the first base of the downstream primer was changed to a degenerate base R; according to the conserved sequence of the KHV ORF7 gene, a pair of specific primers and a TaqMan probe were designed; Use FAM and VIC as the probe reporter group, and BHQ1 as the probe quencher group; second, determine the reaction system and conditions: use 20 μL reaction system, 2×Probe PCR Master Mix 10 μL, upstream and downstream primers and probes The final primer concentration was between 0.2 μmol / L and 0.8 μmol / L, QN ROX reference dye 0.1 μL, template 2.5 μL, and DEPC water to make up 20 μL; the reaction program was: 95°C pre-denaturation for 2 min, 1 cycle; 95°C for 5 s, Anneal at 50-60°C for 31s, 40 cycles.
Owner:北京市水产技术推广站
Dual real-time fluorescence quantitative detection method for CEV (carp edema virus) and KHV (Koi herpesvirus)
ActiveCN109897918AHigh sensitivityStrong specificityMicrobiological testing/measurementDNA/RNA fragmentationCarp edema virusConserved sequence
The invention discloses a dual real-time fluorescence quantitative PCR (polymerase chain reaction) detection method for a CEV (carp edema virus) and a KHV (Koi herpesvirus). The method comprises following steps: step one, synthesis of primers and a TaqMan probe: a primer sequence is optimized according to a CEV P4a gene sequence, first base of an upstream primer is changed into degenerate base W,and first base of a downstream primer is changed into degenerate base R; a pair of specific primers and one TaqMan probe are designed according to KHV ORF7 gene conserved sequence; FAM and VIC are taken as probe reporter groups and BHQ1 is taken as a probe quenching group; step two, determination of a reaction system and conditions: a 20 mu L reaction system is adopted and determined as 10 mu L 2*Probe PCR Master Mix, final concentration of the upstream and downstream primers and the probe primers is in a range of 0.2 mu mol / L-0.8 mu mol / L, a QN ROX reference dye is 0.1 mu L, a template is 2.5mu L and the balance of DEPC water, and the total amount is 20 mu L; the reaction procedure is that predegeneration at 95 DEG C is performed for 2 min, and one cycle is executed; annealing is performed for 5 s at 95 DEG C and performed for 31 s at 50-60 DEG C, and 40 cycles are executed.
Owner:北京市水产技术推广站
DNA vaccine for koi herpes virus (KHV) disease
The present invention provides a DNA vaccine for carps for inducing protective immunity against Koi herpesvirus (KHV). The DNA vaccine comprises a DNA comprising a nucleotide sequence encoding an immunogenic polypeptide against Koi herpesvirus (KHV) of carps, or an expression vector comprising the DNA as an active ingredient.
Owner:TOKYO UNIV OF MANNE SCI & TECH
Bacillus velezensis YFI-4, and application thereof in preparation of drugs for treating viral diseases of freshwater cultured animals
ActiveCN111500488AAvoid infectionNo side effectsBacteriaMicroorganism based processesAnimal virusDisease
The invention relates to the technical field of aquatic antiviral micro-ecological preparations, and especially relates to a bacillus velezensis YFI-4, and application thereof in preparation of drugsfor treating viral diseases of freshwater cultured animals. The preservation number of the bacillus velezensis disclosed by the invention is CCTCC NO: M2019653. The bacillus velezensis YFI-4 disclosedby the invention is used for fermenting supernatant, reduces infection activity of koi herpesvirus (KHV), cyprinid herpesvirus type 2 (CyHV-2), giant salamander iridovirus (GSIV) and the like on hostcells, inhibits infection of virus invasion on the cells is inhibited, and has no toxic or side effect on the cells and can be used for preventing and treating viral diseases of freshwater cultured animals.
Owner:YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Genetic markers for discrimination and detection of viral hemorrhagic septicemia virus causing infectious aquatic organism diseases, and method of discriminating and detecting the virus using the same
Genetic markers are described for discriminating or detecting viruses causing infectious aquatic organism diseases, and a method of discriminating and detecting the viruses using the same is disclosed, in which the method includes selecting and amplifying a DNA nucleotide sequence encoding a gene specific for viral hemorrhagic septicemia virus (VHSV), red sea bream iridovirus (RSIV) or infectious spleen and kidney necrosis virus (ISKNV), which is a virus causing red sea bream iridovirus disease, or Koi herpesvirus (KHV); hybridizing a peptide nucleic acid (PNA) that specifically recognizes the amplification product; controlling the temperature of the hybridization product to obtain a temperature-dependent melting curve; and discriminating the viral type or detecting whether or not fish are infected with the viral type by analyzing the obtained melting curve to determine a melting temperature.
Owner:NAT FISHERY PROD QUALITY MANAGEMENT SERVICE
Koi herpesvirus RPA primer pair, probe and detection kit and application thereof
ActiveCN113046479AHigh sensitivityEasy to detectMicrobiological testing/measurementMicroorganism based processesVirologyBioinformatics
The invention discloses a koi herpesvirus RPA primer pair, a probe and a detection kit and application thereof. Sequences of the RPA primer pair are as shown in SEQ ID NO.4-19, and the sequences of the probe are as shown in SEQ ID NO.2-3. The probe and the primers provided by the invention are high in sensitivity, stable in detection effect and high in specificity. On the basis, the constructed detection reagents and detection kit have very high detection sensitivity, the lowest detection limit is 100 copy / mu L, and the detection time is only 4-18 minutes. Compared with KHV detection gold standard PCR and real-time PCR, the detection reagents and the detection kit have the advantages of time and detection accuracy, with extremely high application value.
Owner:PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
DNA vaccine for Koi herpes virus (KHV) disease
Owner:TOKYO UNIV OF MANNE SCI & TECH
A kind of koi herpes virus cpa detection primer and application
ActiveCN106148565BEfficient detectionStrong specificityMicrobiological testing/measurementDNA/RNA fragmentationWater bathsNucleic acid detection
The invention discloses a KHV (koi herpes virus) CPA (cross priming amplification) detection primer and an application. A KHV CPA detection kit comprises following ingredients: 10*ThermoPol Reaction Buffer, Bst DNA polymerase, dNTPs, cross primers CPF and CPR, detection primers DF and DR, stripping primers DPF and DPR, MgSO4, Betaine and a nucleic acid test strip. The KHV CPA detection primer has the characteristics of simplicity, convenience, rapidness and high specificity and sensitivity and is objective and direct in determination of results, low in cost, convenient to use and very safe to people and the environment. The KHV CPA detection primer not only can be used in a special laboratory, but also can be applied to rapid onsite detection in the field, only one metal bath or water bath kettle is required, and a KHV in a sample can be accurately detected within 1.5 h.
Owner:YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
A kind of bacillus licheniformis yfi-2 and its application in the preparation of freshwater farmed animal virus disease medicine
ActiveCN111440739BAvoid infectionNo side effectsBacteriaMicroorganism based processesAnimal virusDisease
The invention relates to the technical field of aquatic antiviral microecological preparations, in particular to a bacillus licheniformis YFI‑2 and its application in the preparation of freshwater cultured animal virus disease drugs. The preservation number of the Bacillus licheniformis of the present invention is CCTCC NO: M2019652. The fermentation supernatant of Bacillus licheniformis YFI‑2 in the present invention can reduce grass carp reovirus (GCRV), koi herpes virus (KHV), carp herpes virus type 2 (CyHV‑2), channel catfish reo Viruses (CCRV) and giant salamander iridescent virus (GSIV) have infective activity on host cells, inhibit virus invasion and infection of cells, and have no toxic side effects on cells, and can be used to prevent and treat viral diseases in freshwater farmed animals.
Owner:HUBEI HONGWANG ECOLOGICAL AGRI TECH
Carp herpes virus detection kit and detection method thereof
ActiveCN104032037BImprove positive detection rateReduce False Negative TestsMicrobiological testing/measurementBuffer solutionTrace Amounts
The invention relates to a Cyprinid herpesvirus detection kit and a detection method thereof, belonging to the technical field of virus genome detection. The detection kit comprises a 10* reaction buffer solution, a 1U / mu L Taq enzyme, a primer set composed of 10 mu M of P1F and 10 mu M of P1R, a primer set composed of 10 mu M of P2F and 10 mu M of P2R, pure water and a 15 mu g / mL positive DNA. The kit can be used for simultaneously detecting genomes of carp pox herpesvirus, haematopoietic necrosis herpesvirus of goldfish and Koi herpesvirus. The method has the advantages of excellent visuality, favorable sensitivity and high specificity, has favorable effects on detecting trace amounts of Cyprinid herpesvirus genome in the long-storage-time specimen, and can be used for Cyprinid-herpesvirus-infected laboratory detection and Cyprinid herpesvirus molecule epidemiological survey.
Owner:ZHEJIANG INST OF FRESH WATER FISHERIES
Bacillus licheniformis YFI-2 and application thereof in preparation of drugs for treating viral diseases of freshwater cultured animals
ActiveCN111440739AAvoid infectionNo side effectsBacteriaMicroorganism based processesBacillus licheniformisAnimal virus
The invention relates to the technical field of aquatic product antiviral micro-ecological preparations, and in particular relates to bacillus licheniformis YFI-2 and an application thereof in preparation of drugs for treating viral diseases of freshwater cultured animals. The preservation number of the bacillus licheniformis disclosed by the invention is CCTCC NO: M2019652. A fermenting supernatant of the bacillus licheniformis YFI-2 disclosed by the invention can reduce infection activity of grass carp reovirus (GCRV), koi herpesvirus (KHV), cyprinid herpesvirus type 2 (CyHV-2), ictalurus punctatus reovirus (CCRV), giant salamander iridovirus (GSIV) and the like on host cells, can inhibit infection on cells by virus invasion, has no toxic or side effect on cells, and can be used for preventing and treating viral diseases of freshwater cultured animals.
Owner:HUBEI HONGWANG ECOLOGICAL AGRI TECH
A method for constructing a koi brain cell line and its application
ActiveCN105087491BGood growthSensitiveMicroorganism based processesNervous system cellsHistiocyteTGE VACCINE
Owner:PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
Primer set and application thereof for detecting koi herpesvirus sph gene
InactiveCN104152580BStrong specificityHigh sensitivityMicrobiological testing/measurementMicroorganism based processesNucleotideMicrobiology
The invention belongs to the technical field of biology and discloses a primer set for the detection of a koi herpesvirus Sph gene and the application of the primer set. The premier set for the detection of the koi herpesvirus Sph gene comprises a forward outer primer having the nucleotide sequence shown in SEQ ID No.1, a backward outer primer having the nucleotide sequence shown in SEQ ID No.2, a forward inner primer having the nucleotide sequence shown in SEQ ID No.3 and a backward inner primer having the nucleotide sequence shown in SEQ ID No.4. The primer set provided by the invention is high in specificity and sensitivity, and suitable for amplification of koi herpesvirus Sph gene through an LAMP (Loop-Mediated Isothermal Amplification) method, and can be used for preparation of a reagent for detection of the koi herpesvirus Sph gene.
Owner:中国检验认证集团吉林有限公司 +1
Visual rapid detection kit for KHV (Koi herpesvirus)
InactiveCN109097498AShorten the timeEasy to operateMicrobiological testing/measurementDNA preparationWater bathsMagnetic bead
The invention discloses a visual rapid detection kit for KHV (Koi herpesvirus). The visual rapid detection kit comprises a reagent A, a reagent B, a reagent C, a reagent D, a reagent E, a reagent F, areagent G and a detection tube containing a reagent H, wherein the reagent A is a tissue lysate I; the reagent B is a tissue lysate II; the reagent C is isopropyl alcohol; the reagent D is aqueous ethanol with volume percentage concentration being 65%-80%; the reagent E is sterilized double distilled water; the reagent F is 90-110 mg of nucleic acid extracting magnetic beads resuspended in 1 ml of double distilled water; the reagent G is KHV positive plasmid. The operation process of the kit is simple, KHV detection can be completed in 1.5-2 h, and the KHV detection can be completed only by one water bath kettle or metal bath without any high-end instruments and equipment.
Owner:天津市水生动物疫病预防控制中心
Anti-koi herpes virus monoclonal antibody and its cell line and application
ActiveCN108866008BStrong specificityBiological material analysisImmunoglobulins against virusesElisa kitStructural protein
Owner:PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
Biosynthetic gene clusters of pentostatin and vidarabine and their applications
ActiveCN106701788BImprove dynamic characteristicsIncrease productionHydrolasesMicroorganism based processesBiosynthetic genesStreptomyces antibioticus
The invention relates to cloning, sequencing, analysis and functional study of a biosynthetic gene cluster of a natural product pentostatin which is produced from streptomyces antibioticus and is used for treating lymphocytic leukemia and a natural product arabinofuranosyladenine for resisting Koi herpes virus and varicella-zoster virus and application of the biosynthetic gene cluster. Biosynthetic genes of the two compounds are included in the same gene cluster and are independent from each other. The whole gene cluster comprises ten genes, namely three genes related to pentostatin synthesis, five genes related to arabinofuranosyladenine synthesis and two genes related to transportation regulation of the pentostatin and arabinofuranosyladenine. According to genetic manipulations of the previous biosynthetic genes, biosynthesis of the pentostatin or arabinofuranosyladenine can be blocked or improved. The genes provided by the invention and proteins thereof can be used for genetic engineering, protein expression, enzymic catalytic reaction and the like of the compounds, and can be further used for searching and discovering compounds or genes and proteins which can be used for medicines, industry or agriculture.
Owner:WUHAN UNIV
RT-PCR (reverse transcription polymerase chain reaction) Detection kit and method of koi herpesvirus
InactiveCN108384896AIncreased sensitivityBroad differential diagnosisMicrobiological testing/measurementMicroorganism based processesReverse transcription polymerase chain reactionVirus
The invention discloses an RT-PCR (reverse transcription polymerase chain reaction) detection kit and method of koi herpesvirus. A primer group includes primers S1 and S2, wherein S1 is 5'-AAGGCTTAGTAGCTTACCAT-3', S2 is 5'-CAAGGTAGCTGAGTCGACCTATG-3', and S3 is 5'-ATAAGGGCTGAGTCCTGAT-3'. The specific primers S1, S2 and S3 are designed and capable of carrying out specific amplification on koi herpesvirus. The detection kit as a special kit has high sensitivity, reaches 100% in coincidence rate as compared to virus separation, IFA (indirect immunofluorescent assay) and other methods, and is widely applicable to identification diagnosis of koi herpesvirus.
Owner:张朝明 +3
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