30results about How to "No need for electrophoretic detection" patented technology

The invention dislcoses an RAA (recombinase-aid amplification) constant-temperature fluorescence detection method and a detection kit for EHP (enterocytozoon hepatopenaei). The detection kit comprisesa forward primer SEQ ID NO. 1, a reverse primer SEQ ID NO. 2, a specific fluorescence probe SEQ ID NO. 3, reaction liquid, recombinant polymerase and a reference substance. The kit has the advantagesthat the specificity is strong; the detection sensitivity is high, and can reach 100fg/mu L; high accuracy and reliability are achieved; the operation is simple and rapid, and the kit is suitable forfield detection and has wide application scenarios.

The invention discloses a recombinase-aid amplification (RAA) thermostatic fluorescence detection method of infectious hypodermal and hematopoietic necrosis virus (IHHNV) of prawns, and a detection kit. The detection kit comprises a forward primer shown in SEQ ID No. 1, a reverse primer shown in SEQ ID No. 2, a specific fluorescent probe shown in SEQ ID No. 3, a reaction solution, recombinant polymerase and a reference substance. The kit provided by the invention is high in specificity; the detection sensitivity is high, and can reach up to 0.32fg/mu L; the detection method has the advantagesof high accuracy, reliability, simple, convenient and fast operation, and suitability for field detection, thus having wide application prospect.

The invention discloses a RAA constant temperature fluorescence detection method and a detection kit for a salmonid alphavirus (SAV). The detection kit comprises a forward primer SEQ ID NO.1, a reverse primer SEQ ID NO.2, a specific fluorescent probe SEQ ID NO.3, a reaction liquid, reverse transcriptase, recombinant polymerase and a reference substance. The kit disclosed by the invention is high in specificity, high in detection sensitivity which can reach 1.93 fg/[mu]L, high in accuracy, reliable, simple and rapid to operate and suitable for field detection, and has wide application scenes.

The invention discloses an RAA constant-temperature fluorescence detection method and kit for infectious spleen and kidney necrosis virus (ISKNV) in mandarin fishes. The detection kit comprises a forward primer SEQ ID NO.1, a reverse primer SEQ ID NO.2, a specific fluorescent probe SEQ ID NO.3, a reaction solution, recombinant polymerase and a reference substance. The kit disclosed by the invention is strong in specificity, high in detection sensitivity which can reach 300 fg/[mu]L, high in accuracy, reliable, simple, convenient and rapid to operate, suitable for field detection and wide in application prospect.

The invention discloses an RAA constant-temperature fluorescent detection method and detection kit for viral haemorrhagic septicemia (VHSV). The detection kit comprises a forward primer SEQ ID NO.1, areverse primer SEQ ID NO.2, a specific fluorescent probe SEQ ID NO.3, a reaction solution, reverse transcriptase, recombinant polymerase and a reference substance. The kit has high specificity and high detection sensitivity reaching 10fg/[mu]L, is high and reliable in accuracy, is simple and quick to operate, is suitable for field detection, and has an extensive application prospect.

The invention discloses a loop-mediated isothermal amplification primer for rapidly detecting rotylenchulus reniformis and an application of the loop-mediated isothermal amplification primer. A loop-mediated isothermal amplification primer group is designed with a 28S rDNA-D2D3 region as a target sequence and comprises a primer pair RrF3/RrB3 and a primer pair RrFIP/RrBIP; the primer sequences areshown in SEQ ID NO. 1-4; a loop-mediated isothermal amplification detection method is further established based on the primer group. The detection method has high specificity, high sensitivity, goodrepeatability and wide detection population range for rotylenchulus reniformis, has low requirements for instruments and detection personnel, has visual reaction result, is accurate, reliable, safe, simple, rapid and low in cost, provides technical support for detection of rotylenchulus reniformis, especially inspection and quarantine work, is very suitable for rapid detection at the grass-roots level, and has quite good popularization and application value.

The invention provides a method for detecting American ginseng species in traditional Chinese medicine ( Panax quinquefolius ) Recombinase-aid Amplification (RAA) constant temperature detection method and detection kit. The detection kit includes a forward primer of SEQ ID NO.1, a reverse primer of SEQ ID NO.2, a specific fluorescent probe of SEQ ID NO.3, a reaction solution, a recombinant polymerase and a control substance. The kit of the invention has strong specificity; high detection sensitivity, which can reach 0.10fg/μL; high accuracy and reliability; simple and quick operation, suitable for on-site detection, and has wide application scenarios.

The invention discloses a loop-mediated isothermal amplification (LAMP) primer group for rapidly detecting pratylenchus penetrans. The primer group comprises an outer primer pair peF31/peB31 and an inner primer pair peFIP1/peBIP1, wherein the sequences of the primers are respectively shown in SEQ ID NO.1-4. An LAMP method is established based on the primer group and is characterized by using deoxyribonucleic acid (DNA) of samples as a template to carry out LAMP. The results can be judged by the following two methods after reaction ends: one method is that amplification products undergo electrophoresis and the samples with specific ladders are judged to be positive; the other method is that the samples having color change observed with the naked eye are positive by adding SYBR green I to a reaction system. The LAMP method has low requirements for instruments and equipment, is rapid and safe, has good specificity and strong sensitivity, provides technical support for detection of pratylenchus penetrans, especially pratylenchus penetrans rapid detection work of grassroots quarantine units, and has good popularization and application values.

The invention discloses a loop-mediated isothermal amplification primer set for identifying three kinds of pratylenchus pratensis on sugarcane and a kit comprising the loop-mediated isothermal amplification primer set. The loop-mediated isothermal amplification primer set comprises three groups of primers, namely, an outer primer pair PZF3/PZB3 and an inner primer pair PZFIP/PZBIP, an outer primer pair PPF3/PPB3 and an inner primer pair PPFIP/PPBIP, as well as an outer primer pair PBF3/PBB3 and an inner primer pair PBFIP/PBBIP, wherein the nucleotide sequences of the primers are respectively shown as SEQ ID NO.1-12. The three kinds of pratylenchus pratensis are pratylenchus zeae, P. parazeae and P. brachyurus. The loop-mediated isothermal amplification primer set and the kit comprising the loop-mediated isothermal amplification primer set have low requirements on instrument and equipment, are simple, rapid and safe, and are good in specificity and high in sensitivity, and a technical support is provided for the detection for pratylenchus zeae, P. parazeae and P. brachyurus, especially, the rapid identification and distinguishing for the three kinds of pratylenchus pratensis of the grassroots units.