The invention discloses a Pc-VAP
gene, a
protein coded by the Pc-VAP
gene and application of the Pc-VAP
gene to regulation and control of infection capacity of
pratylenchus coffee, and belongs to the technical field of
biology. The Pc-VAP is located in esophageal glands, intestinal tracts, gonads and eggs of
Pratylenchus coffee. RT-qPCR results show that the Pc-VAP is expressed in different
insect states of the
pratylenchus coffee, and the expression quantity of female
pratylenchus coffee is the highest. After the Pc-VAP is silenced by the
in vitro RNAi, the expression quantity of the Pc-VAP gene in the
nematode body is obviously reduced (Plt; 0.05), the
infectivity of the nematodes is obviously reduced; after the Pc-VAP
gene silencing is mediated by the VIGS, the
pathogenicity of the pratylenchus coffee to the host is obviously reduced. The results show that the Pc-VAP gene can be used as a
target gene and has important application potential in prevention and control of pratylenchus coffee.