Porcine parvovirus strain

A parvovirus and strain technology, which is applied in the field of preparation of porcine parvovirus strains, can solve the problems of severe PPV infection and economic losses in the pig industry

Active Publication Date: 2013-12-11
HENAN AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0003] Some scientific researchers have conducted epidemiological investigations on porcine parvovirus in Henan, and found that many pig farms have PPV antib

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Embodiment Construction

[0014] A porcine parvovirus strain, Parvoviridae, CCTCC NO: V201313.

[0015] A study on the isolation, identification and biological characteristics of porcine parvovirus strain HP104

[0016] 1 Materials and methods

[0017] 1.1 Materials

[0018] 1.1.1 Diseased tissue and cell lines

[0019] The disease tissue was collected from the placenta, liver, spleen, lung and lymph nodes of dead piglets in a pig farm in Xinxiang City, Henan Province. There were 1,000 sows in a pig farm in Xinxiang City, Henan Province. In September 2012, 10 primiparous sows suffered from abortion or stillbirth. The sows had no obvious symptoms, while the weaned piglets had rough coat and depressed spirit. According to the onset, suspected to be PPV infection), porcine testis (ST) cells were purchased from China Veterinary Drug Control Institute (cryopreserved).

[0020] 1.1.2 Preparation of HA reagent

[0021] Preparation of 0.01 M PBS solution: Take 8 g of sodium chloride, 0.2 g of potassium ch...

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Abstract

The present invention discloses a porcine parvovirus strain, which is named Parvoviridae, and has the preservation number of CCTCC NO:V201313. According to the present invention, a PCR method is adopted to carry out PCR detection on suspected-PPV disease materials collected in the Henan region, inoculation on porcine testis (ST) cells is performed, isolation is performed to obtain a strain of virus named HP104, and results of cytopathic effect observation, biological characteristic research, virus shape observation through electron microscopy and other approaches confirm that the isolated virus is the porcine parvovirus; and the porcine parvovirus HP104 virus solution and an oil emulsion are mixed to prepare an emulsifier, the emulsifier is inoculated on health guinea pigs, a HI antibody level achieves 128 after 28 days, and porcine parvovirus antibody in the control group guinea pigs is negative, such that the results show that the Henan isolated strain PPVHP104 has good immunogenicity.

Description

technical field [0001] The invention relates to a preparation method of porcine parvovirus strain. Background technique [0002] Porcine parvovirus (PPV) is one of the main pathogens causing reproductive disorders in sows. It can cause stillbirth, malformed, mummified and sickly piglets in infected sows, as well as piglet dermatitis symptoms, non-suppurative myocarditis and wasting syndrome. Porcine parvovirus is often co-infected with porcine pseudorabies virus, porcine circovirus, swine fever, porcine reproductive and respiratory syndrome virus, etc., which aggravates its harm. [0003] Some scientific researchers have conducted epidemiological investigations on porcine parvovirus in Henan, and found that many pig farms have PPV antibody-positive pigs, indicating that PPV infection in Henan is relatively serious, which has caused major damage to the pig industry in Henan. Economic losses. Under the current circumstances, it is particularly important to study porcine par...

Claims

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Application Information

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IPC IPC(8): C12N7/00C12R1/93
Inventor 崔保安陈红英吴宇阳陈龙彪魏战勇李新生
Owner HENAN AGRICULTURAL UNIVERSITY
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