Method by utilizing rabbit-origin cells to produce swine fever live vaccine

Abstract

The invention relates to a method by utilizing rabbit-origin cells to produce swine fever live vaccine, which comprises the following steps that: selecting cells for producing vaccine, preparing cells for producing vaccine, breeding cell virus strain, breeding venom for producing vaccine, preparing vaccine, split charging and freeze drying. The method is characterized in that: rabbit-origin susceptible cells are used for substituting bovine testicular cells or goat kidney cells to produce swine fever live vaccine, which can solve the pollution problem of external virus from cow and sheep, and by strictly controlling the raw materials and the culture conditions, the purity of the produced vaccine can be guaranteed, and the safety and the effect of the vaccine can be guaranteed; and the rabbit-origin susceptible cells to substitute the transferred cells to manufacture the swine fever live vaccine, so the potential hazard that the transferred cells can cause cancer can be avoided, and the safety of the vaccine is higher. The swine fever cell venom cultured through the method has high content of virus, the antigen tilter is 10 times or more of that of the present vaccine, the immune efficacy of the vaccine can be greatly improved, and the weaknesses of the present vaccine in the clinical use can be overcome.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, in particular to a method for producing live swine fever vaccine with rabbit-derived cells. Background technique [0002] Classical swine fever virus (CSFV) is a serious animal virus, which causes huge economic losses to the pig industry every year. At present, attenuated live vaccine immunization is mainly used in the world to prevent and control swine fever, so as to control the prevalence of swine fever. The attenuated CSF vaccine strain (strain C) propagated in heterologous primary cells is currently the main attenuated vaccine in my country. C strain is a vaccine strain with good immune protection that was bred from rabbits in the 1950s in my country. Because the vaccine strain is stable, safe and protective, it is recognized as the best and most widely used vaccine strain in the world. Due to the needs of application, the vaccine production of CSFV in my country ha...

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Problems solved by technology

[0003] Because the infection rate of viral diarrhea virus (BVDV) in my country's cattle herd is very high, almost 100%, so the production of swine fever vaccine with bovine testicular cells is easy to cause cell exogenous virus contamination, and the virus titer is not high. Unstable, large differences between vaccine batches, seriously affecting the quality of the vaccine; similarly, the use of sheep kidney cells to produce swine fever vaccine, due to the possible presence of border virus (BDV) in the flock, also has the same hidden danger

Bovine viral diarrhea virus (BVDV), sheep border virus (BDV) and swine fever virus (HCV) all belong to the genus Pestivirus in the family Flaviviridae. There is a close antigenic and serological relationship between the three viruses, and they can induce each other. A certain degree of homologous virus antibody, after immunizing pigs with a swine fever cell vaccine contaminated with BVDV or BDV, BVDV or BDV will interfere with the immunity of the swine fever vaccine, inhibit the production of swine fever antibodies, and cause severe s consequence

[0004] Some domestic research institutes and animal vaccine manufacturers try to use cell lines to produce swine fever vaccines, which can avoid foreign virus contamination and improve the immune effect in a certain way, but the passaged cells have the possibility of tumorigenesis, so they are used in the production of swine fever vaccines Potential food safety hazard

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