Method for establishing exopalaemon carinicauda family by applying in-vitro egg culture

A technique for in vitro culture of white shrimp, applied in the field of white shrimp, can solve the problems of high survival rate and hatching failure rate of juvenile shrimp, and achieve the effects of reducing the rate of residual juvenile shrimp, improving the hatching rate and reducing the probability of hatching failure.

Active Publication Date: 2014-08-06
INST OF OCEANOLOGY & MARINE FISHERIES JIANGSU
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The current method for establishing a family of spinytail shrimp has the dis

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0020] Select broodstock individuals with larger individuals, stronger vitality, and more eggs, and sterilize them in 0.0002 mg / ml potassium permanganate seawater solution for 5 minutes; The eggs are gently peeled off from the broodstock individual, with moderate strength, and it is advisable not to damage the shrimp eggs. Take the eggs separated from a single white prawn broodstock, soak them in 0.0002 mg / ml potassium permanganate seawater solution for 2 minutes, rinse twice with seawater with a salinity of 25, and transfer to a container with 30 mL, salinity 25 in a sterilized seawater glass petri dish (150mm in diameter); place the glass petri dish with shrimp ovum in a temperature-controlled shaker for vibration culture, the temperature is set at 20°C, and the oscillation speed is 120 rpm; According to the time points of 4h, 10h, 20h, 48h, 72h, 144h, 198h, 240h, and 300h, the development of shrimp eggs was observed microscopically to check for abnormal development. Fresh ...

Embodiment 2

[0022] Select broodstock individuals with larger individuals, stronger vitality, and more eggs, and sterilize them in 0.0002 mg / ml potassium permanganate seawater solution for 5 minutes; The eggs are gently peeled off from the broodstock individual, with moderate strength, and it is advisable not to damage the shrimp eggs. Take the eggs separated from a single white prawn broodstock, soak them in 0.0002mg / ml potassium permanganate seawater solution for 3 minutes, wash them with seawater with a salinity of 25 for 3 times, and transfer them to a container with 40mL, salinity 25 sterilized seawater glass petri dishes (150 mm in diameter); place the glass petri dishes with shrimp ovum in a temperature-controlled shaker for oscillation culture, set the temperature to 25°C, and the oscillation speed to 140 rpm; According to the time points of 4h, 10h, 20h, 48h, 72h, 144h, 198h, 240h, and 300h, the development of shrimp eggs was observed microscopically to check for abnormal developm...

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Abstract

The invention relates to a method for establishing an exopalaemon carinicauda family by applying in-vitro egg culture. The method comprises the steps that parent exopalaemon carinicauda are selected and disinfected, and the parent exopalaemon carinicauda and eggs are separated; shaking culture parameters of the eggs are measured; microscopic observation is performed in the culture process; the progressive decreasing control of salinity is performed in the culture process; young exopalaemon carinicauda after incubation are cultured. According to the method, the probability of incubation failure caused by influence of different factors in the egg laying process of the parent exopalaemon carinicauda can be effectively lowered, the incubation rate of the eggs and the survival rate of the young exopalaemon carinicauda are increased, and therefore the method has good application prospects in the application of establishing the exopalaemon carinicauda family.

Description

technical field [0001] The invention belongs to the field of white prawns, in particular to a method for establishing a family of white prawns by culturing shrimp eggs in vitro. Background technique [0002] Exopalaemon carinicauda belongs to true shrimps. Its reproductive method is essentially different from that of prawns. It belongs to ovipositor reproduction. After mating, the fertilized eggs are held on the abdominal limbs for development, and the continuous swinging of the abdominal limbs is used to provide enough for embryonic development. Oxygen, the larvae leave the mother after hatching, and the hatched larvae are daphnia. [0003] Family selection is one of the methods of selective breeding. The family selection of aquaculture animals is difficult due to its breeding characteristics and reproductive biological characteristics. Among them, the family selection of fish has made the best progress and has achieved great results. results. However, the family selectio...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 沈辉万夕和乔毅黎慧徐献明王李宝凌云孙瑞健史文君
Owner INST OF OCEANOLOGY & MARINE FISHERIES JIANGSU
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