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Sperm freezing fluid and preparation method thereof

A freezing solution and sperm technology, applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problems of insufficient preservation of biopsy testicular tissue, unable to effectively preserve sperm motility and integrity, etc., to ensure safety. , the effect of protecting the integrity and protecting the activity

Pending Publication Date: 2018-04-20
瑞柏生物(中国)股份有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

Conventional sperm freezing solution is egg yolk solution buffered with HEPES or MOPS saline solution, which cannot effectively preserve the vitality and integrity of sperm, especially for biopsied testicular tissue.

Method used

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Embodiment Construction

[0025] The present invention will be further described below in combination with specific implementation methods.

[0026] The sperm freezing solution of the present invention is prepared by dissolving glycerin at a concentration of 20-30% in a saline solution containing sucrose, antibiotics and indicators.

[0027] The concentration of the sucrose is 0.1-0.3mol / L; the antibiotic is gentamicin, the concentration is 5-11μg / ml; the indicator is phenol red, the concentration is 9-10μg / ml.

[0028] The saline solution is a compound culture solution using HEPES or MOPS as a buffer, based on sodium, potassium, magnesium, and calcium ions, and using glucose, sodium pyruvate, sodium lactate, and albumin as energy substances; it contains: Sodium 98.11-102.00mmol / L, potassium chloride 4.55-4.70mmol / L, magnesium sulfate 0.18-0.22mmol / L, calcium chloride 1.92-2.12mmol / L, sodium bicarbonate 3.90-4.10mmol / L, HEPES or MOPS19 .99-22.00mmol / L, glucose 2.68-2.88mmol / L, sodium pyruvate 0.31-0.3...

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Abstract

The invention relates to a sperm freezing fluid and a preparation method thereof and belongs to the technical field of human assisted reproduction. The sperm freezing fluid is prepared by dissolving glycerinum into a salt solution with cane sugar, antibiotics and an indication agent in a concentration of 20-30%. Due to addition of the glycerinum and the cane sugar in the sperm freezing fluid provided by the invention, the glycerinum and the cane sugar are adopted as cell protection agents which can not only gently and effectively protect activity of sperms and completeness of DNA (Deoxyribonucleic Acid), but also protect the activity of testicular tissue cells, furthermore the activity of sperms in testicular testes is protected, the fertility rate of the sperms is increased, and the security of assisted reproductive technologies is powerfully ensured.

Description

technical field [0001] The invention relates to a sperm freezing liquid and a preparation method thereof, belonging to the technical field of human assisted reproduction. Background technique [0002] In human assisted reproductive technology, spermatozoa and testicular biopsy tissue are frozen to preserve sperm motility and plasma membrane integrity. Conventional sperm freezing solution is egg yolk solution buffered with HEPES or MOPS saline solution, which cannot effectively preserve the vitality and integrity of sperm, especially for biopsy testicular tissue. Contents of the invention [0003] The object of the present invention is to provide a sperm freezing solution with various quality control standards, which can not only effectively improve the vitality of sperm, improve the success rate of artificial insemination (IUI) of frozen sperm, but also provide a guarantee for the safety of assisted reproductive technology. a strong guarantee. [0004] In order to solve ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0215A01N1/0221
Inventor 冯怀亮
Owner 瑞柏生物(中国)股份有限公司
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