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Biocontrol preparation and application thereof in prevention and treatment of pratylenchus coffea

A technology of biocontrol agent and bacterium, applied in the field of biocontrol agent and its application in the prevention and control of brevis nematode coffee, can solve the problems of biocontrol agents that cannot provide control effect to prevent and control brevis nematode coffee, and achieve good toxicity kill effect

Active Publication Date: 2021-04-13
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the deficiencies in the prior art, the technical problem to be solved by the present invention is to overcome the problem that the prior art cannot provide a biocontrol agent for the prevention and treatment of the brevium coffee nematode with a better control effect, and propose a biocontrol agent that can be used alone to treat A biocontrol agent with good control effect on Brachybody coffee nematode and its application in the control of Brachybody coffee nematode

Method used

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  • Biocontrol preparation and application thereof in prevention and treatment of pratylenchus coffea
  • Biocontrol preparation and application thereof in prevention and treatment of pratylenchus coffea
  • Biocontrol preparation and application thereof in prevention and treatment of pratylenchus coffea

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Experimental program
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Effect test

Embodiment 1

[0028] Isolation and identification of Paenibacillus polymyxa J2-4

[0029] 1. Strain isolation

[0030] Severely diseased ginger and healthy ginger were excavated from plots with severe root-knot nematode incidence in Qingdao, Shandong, cut into thin slices, mixed evenly, took 5g, rinsed with sterile water for 3 times, then sterilized with 1% sodium hypochlorite for 5 minutes, and then disinfected with sterile water. Rinse with bacterial water for 3 times, put it into a sterilized mortar, add quartz sand and grind to obtain a suspension, shake the suspension evenly, take 1mL and add it to a test tube filled with 9mL sterile water, mix well, and then gradually dilute to 10 -2 -10 -6Concentration, take 100 μL of the dilution solution of each concentration and spread it evenly on the LB medium, apply 3 plates for each concentration, and culture at a constant temperature of 37°C for 2-3 days, pick a single colony according to the morphological characteristics of the colony, and ...

Embodiment 2

[0039] Preparation of microbial nematicide

[0040] 1. Pick a single colony of Paenibacillus polymyxa J2-4 and inoculate it into LB liquid medium (5g / L yeast extract, 10g / L peptone, 10g / L sodium chloride; pH 7.0), at 37°C, without Illuminated, 200rpm / min shaking culture for 48h, to obtain a fermentation broth with OD600nm=2.0.

[0041] 2. Centrifuge the fermented liquid obtained in step 1 at a speed of 8000 rpm / min for 20 min, and the collected supernatant is the microbial nematocide.

Embodiment 3

[0043] Toxic Effect of Paenibacillus polymyxa J2-4 Aseptic Fermentation Filtrate on Brevibodia coffee nematode

[0044] 1. Cultivation and preparation of the tested nematodes: the tested coffee short-bodied nematodes (Pratylenchus coffeeae) were preserved and multiplied by the Plant Nematode Laboratory of Qingdao Agricultural University.

[0045] 2. In a sterile 24-well cell culture plate, add 1mL of the sterile supernatant prepared in Implementation 2 to each well of the experimental group, add the same amount of sterile water to the control group, and pick 50 Brachyspira coffee nematodes into each well The 2nd instar larvae were cultured at a constant temperature of 28°C, and after 24 hours and 48 hours of culture respectively, the corrected mortality rate of nematodes was calculated. Because the nematodes had suspended animation, 1 mol / L NaOH solution was added dropwise to each treatment before counting the mortality to stimulate the recovery of the suspended nematodes, and...

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Abstract

The invention discloses a biocontrol preparation and application thereof in prevention and treatment of pratylenchus coffea, and belongs to the technical field of microbial pesticides. According to the technical scheme, the biocontrol preparation comprises active ingredients including paenibacillus polymyxa J2-4 and / or a culture and / or metabolite of the paenibacillus polymyxa J2-4; and the paenibacillus polymyxa J2-4 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 21515. The biocontrol preparation is applied to prevention and treatment of the pratylenchus coffea, the problem that in the prior art, a biocontrol preparation which is good in prevention and treatment effect and used for preventing and treating the pratylenchus coffea cannot be provided is solved, and the biocontrol preparation has the advantage that the good prevention and treatment effect on the pratylenchus coffea can be achieved when the biocontrol preparation is used independently.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a biocontrol agent and its application in preventing and treating Brachyphyllum coffee nematode. Background technique [0002] Pratylenchus coffeea is not only an important plant pathogenic nematode, but also a quarantine nematode of the genus Pratylenchus. In our country, the brevophilus coffee nematode is mainly distributed in Jiangsu, Shandong, Anhui, Henan and other places, harming yam, kidney bean, cabbage, cauliflower, onion, pea, sweet potato and tomato. The short-bodied nematode coffee damages the host seed potatoes and young roots in the early stage, and mainly damages the tubers in the later stage, causing the tubers of the host plants to become smaller, light brown lesions appear on the damaged roots, and cause root rot, which seriously affects the yield and quality of the host. At present, the prevention and control of coffee brevis nematode ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/25A01P5/00C12R1/01
CPCC12N1/20A01N63/25
Inventor 宋雯雯史倩倩赵洪海梁晨段方猛
Owner QINGDAO AGRI UNIV
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