Genome-wide analysis of APA based on 3t-seq
A genome-wide, wide-ranging analysis technology, applied in the field of RNA PAS detection, which can solve the problems of inability to completely eliminate polyA, large RNA damage and loss, and complicated RNA level operations.
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[0024] The buffer composition used in the specific embodiment is as follows:
[0025] Reagent storage mother solution required for preparing buffer solution (can be purchased from a commercial company, or purchase related reagents to prepare by yourself):
[0026]
[0027] Binding buffer 1 (20ml):
[0028] DEPC treated water 10ml
[0029] 5M NaCl 8ml
[0030] 0.5M EDTA (PH 8.0) 2ml
[0031] Binding buffer 2 (20ml):
[0032]
[0033]
[0034] Wash buffer A (20ml):
[0035]
[0036] Wash buffer B (20ml):
[0037]
[0038] Wash buffer C (20ml):
[0039]
[0040] Wash buffer D (20ml):
[0041]
[0042] The mother solutions for the following washing buffers are all derived from the products of relevant commercial companies, and the mother solutions need to be purchased from the relevant companies to prepare according to the following formula. It is recommended to prepare freshly for each experiment:
[0043] Wash Buffer 1 (400 μl):
[0044] DEPC treate...
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