Pseudobagrus sperm preservation solution and preparation method thereof

Abstract

The invention relates to the field of fish sperm preservation solutions, in particular to a pseudobagrus sperm preservation solution for solving the problem of pseudobagrus large scale reproduction and a preparation method of the pseudobagrus sperm preservation solution. The sperm preservation solution is prepared from sodium chloride, potassium chloride, calcium chloride, magnesium chloride, sodium bicarbonate, glucose and penicillin and is prepared by weighing the raw materials, adding the raw materials in a plastic bottle or a glass bottle, and preparing into a solution with distilled water. The prepared sperm preservation solution is subjected to sperm chromatin detection with a flow cytometer, spermatids are circled on an FSC / SSC graph, and a door is set on an FL1(FITC) / FL3(ECD) scatter diagram for analysis; and a DNA fragment index at is calculated through a formula to evaluate sperm DNA quality. The sperm preservation solution provided by the invention can be preserved for 10 days in a refrigeration tank at the temperature of 4 DEG C, 98 percent or more of sperms keep exuberant vigor and can still be taken as sperms for semination, and the fertilization rate is 95 percent orabove.

Description

technical field [0001] The invention relates to the field of fish sperm preservation solution, in particular to a semen preservation solution for semen to solve the large-scale reproduction of semen and a preparation method thereof. Background technique [0002] In the artificial breeding of catfish, it is necessary to kill the fish to extract the sperm. This artificial breeding process is not suitable for large-scale breeding, because large-scale breeding needs to inseminate hundreds of female fish at a time. If it is too late to kill the male fish temporarily, if the male fish is killed in advance Because the sperm viability is only 15-25 seconds if there is no preservation solution, obviously it will not work. Contents of the invention [0003] The object of the present invention is to overcome the deficiencies in the prior art and provide a sperm preservation solution and a preparation method thereof that can store sperm in a refrigerator at 4°C for 10 days and have st...

AI Technical Summary

Problems solved by technology

[0002] In the artificial breeding of catfish, it is necessary to kill the fish to extract the sperm. This artificial breeding process is not suitable for large-scale breeding, because large-scale breeding needs to inseminate hundreds of female fish at a time. If it is too late to kill the male fish temporarily, if the male fish is killed in advance Because the sperm motility is only 15-25 seconds if there is no preservation solution, it is obviously not enough