The invention belongs to the technical field of
crop genetic breeding, and particularly relates to a broomcorn millet regulatory
gene Hd1 development-based codominant molecular marking method, which comprises the following specific steps: acquiring an Hd1
gene sequence of a paddy rice related species from an NCBI
database, comparing BLAST with a broomcorn millet
reference genome, positioning a broomcorn millet Hd1 homologous region, and identifying the broomcorn millet regulatory
gene Hd1 homologous region. By sequencing Hd1 genes of 20-30 parts of
broom corn millet varieties, combining with generic
genome data, identifying low-frequency PAVs and TE derivative variation, supplementing the defects of traditional SNP /
InDel, preferentially developing markers of a
coding region and a
promoter region, and directly influencing gene functions by variation of the regions, the
polymorphism detection efficiency is improved; cross-species
verification is carried out by using Hd1 markers of related species such as rice and millet, a
broom corn millet marker is rapidly developed by adjusting primers and amplification conditions, a
differential expression region of Hd1 is identified through
transcriptome sequencing, and an EST-SSR or
InDel marker is designed.